Mechanical Strain Tightly Controls Fibroblast Growth
Factor-2 Release From Cultured Human Vascular Smooth
Muscle Cells
by George C. Cheng, William H. Briggs, David S. Gerson, Peter Libby, Alan J.
Grodzinsky, Martha L. Gray, and Richard T. Lee
Circulation Research
Volume 80(1):28-36
January 1, 1997
Copyright © American Heart Association, Inc. All rights reserved.
Schematic diagram of device to apply homogeneous and uniform biaxial strain to monolayer
human vascular smooth muscle cell culture.
George C. Cheng et al. Circ Res. 1997;80:28-36
Copyright © American Heart Association, Inc. All rights reserved.
Radial (A) and circumferential (B) membrane strains as functions of radial position on the
membrane for platen displacements of 3.1, 5.4, 7.3, 9.4, and 17 mm (n=3 to 4 for each
measurement; error bars denote 1 SD).
George C. Cheng et al. Circ Res. 1997;80:28-36
Copyright © American Heart Association, Inc. All rights reserved.
Cumulative fibroblast growth factor-2 (FGF-2) in culture media during continuous dynamic strain
(1 Hz, 14% amplitude, ▪) of human vascular smooth muscle cells, relative to control (•) (n=3 to 7
for each measurement; error bars denote 1 SD).
George C. Cheng et al. Circ Res. 1997;80:28-36
Copyright © American Heart Association, Inc. All rights reserved.
The dependence of release of fibroblast growth factor-2 (FGF-2) from human vascular smooth
muscle cells on the number of cycles of mechanical strain (1 Hz, 14% amplitude).
George C. Cheng et al. Circ Res. 1997;80:28-36
Copyright © American Heart Association, Inc. All rights reserved.
The dependence of release of fibroblast growth factor-2 (FGF-2) from human vascular smooth
muscle cells on the frequency of mechanical strain (90 cycles, 14% amplitude).
George C. Cheng et al. Circ Res. 1997;80:28-36
Copyright © American Heart Association, Inc. All rights reserved.
The dependence of fibroblast growth factor-2 (FGF-2) release from human vascular smooth
muscle cells on the amplitude of mechanical strain (90 cycles, 1 Hz).
George C. Cheng et al. Circ Res. 1997;80:28-36
Copyright © American Heart Association, Inc. All rights reserved.
Dependence of cellular injury on mechanical strain.
George C. Cheng et al. Circ Res. 1997;80:28-36
Copyright © American Heart Association, Inc. All rights reserved.
Effect of pretreatment with heparin on mechanical release of fibroblast growth factor-2 (FGF-2)
by human vascular smooth muscle cells.
George C. Cheng et al. Circ Res. 1997;80:28-36
Copyright © American Heart Association, Inc. All rights reserved.
Ability of heparin to increase fibroblast growth factor-2 (FGF-2) in the culture media after
mechanical strain.
George C. Cheng et al. Circ Res. 1997;80:28-36
Copyright © American Heart Association, Inc. All rights reserved.