Bio200 POGIL Cell Biology Activity 2 – Enzymes
STUDENT KEY – to selected answers
Schivell
1. Protein
2. It has been cleaved (cut) into two parts.
8. An ionic bond (between the O- of the phosphate group in RNA and the Lys41 –NH3+)
and hydrogen bonds (various)
9. a. phosphorus b. higher
10. a. The histidines donate or accept protons to or from the substrate to facilitate the changes in the
substrate's chemical structure.
b. The lysine is helping keep the substrate in the active site through an ionic bond with phosphate.
12. It slows down.
13. a. Yes
b. Mut1
14. dramatically
15. Yes, if Lys41 isn't there, the substrate doesn't stay in the active site as long and not as many
reactions are catalyzed.
On your own:
1. carbohydrate
2. B
3.
4. – spontaneous
5. HIS accepts a proton from Cys to facilitate its new bond with the substrate.
6. an "H" (in actuality, a proton and two electrons)
7. No
9. ΔG‡ (EA)
10. If Cys were changed to valine, the reaction would slow because valine would not be able
to form a covalent bond with the substrate.
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