HIGH THROUGH-PUT RESPIRATION ASSAY FOR SMALL AQUATIC ORGANISMS Use this multi channel system for measuring oxygen consumption inside 80-1700 µL gas tight glass chambers for small aquatic organisms like individual daphnia, zebrafish larvae, embryos, eggs etc. Toldboden 3, 2nd floor · DK-8800 Viborg Phone: +45 3360 2545 [email protected] MICROPLATE WELL DIMENSIONS ID (mm) DEPTH (mm) VOLUME (µL) 4.5 5 80 6 7 200 500 8 10 10 12 940 12 15 1700 FEATURES SYSTEM COMPONENTS •Dual reference optical fluorescence O2sensing technology •Real-time O2 monitoring in each independent chamber •Non-invasive & non-destructive measurements •Reusable oxygen sensor spots •Experimental temperature range 5-40°C •Connect 1-10 readers (24-240 chambers) to a single PC •24-channel microplate oxygen reader •24 oxygen sensor spots •Glass microplate with 24 chambers •Plastic caps with PTFE seals •Water bath for temperature control •Splitter and cable for PC communication •Software for Windows www.loligosystems.com 1201_Loligo-handout_21x21cm.indd 3 13/05/16 08:15 HIGH THROUGH-PUT RESPIRATION ASSAY FOR SMALL AQUATIC ORGANISMS Toldboden 3, 2nd floor · DK-8800 Viborg Phone: +45 3360 2545 [email protected] GLASS CHAMBER MICROPLATES • Plates with 24 chambers • Multiple chamber sizes • Each chamber fitted with oxygen sensor spot • Gas-tight chamber sealing • Clean and sterilize with ethanol • Reusable sensor spots Specifications table for microplate reader system Specifications Compatible oxygen sensor Oxygen channels Power supply Power adapter Communication interface Dimensions [mm] Weight [g] Windows version Oxygen unit Microplate reader system Sensor spots 24 18-24 V DC 100-240V AC in; 18-24 V DC out RS232 163 x 89 x 22 380 XP, Vista, WIN7, WIN10 % oxygen saturation, % air saturation, kPa, Torr, mg/L, mmol or ml/L. 0 – 50 % oxygen saturation 0 – 235 % air saturation 0 – 50 kPa 0 – 235 Torr 0 – 22,5 mg/L 0 – 700 mol 0 – 22,5 ml/L ± 0.4 % O2 at 20.9 % O2 ± 4 hPa at 207 hPa ± 5 mol at 283.1 mol ± 2 % air saturation at 100 % air saturation ± 1 % O2 at 20.9 % O2 ± 5 % air saturation at 100 % air saturation < 0.2 % O2 within one week < 1 % air saturation within one week 5 – 45 °C < 30 sec. Measurement range Resolution Precision Drift at 0 % oxygen (sampling interval 10 min) Measurement temperature range Response time (T90) Sensor properties Compatibility Cross-sensitivity to Cleaning procedures Calibration MINI Aqueous solutions, ethanol, methanol Reduced to ionic strength (salinity); high concentration of small fluorescent molecules in the visible range can interfere 3 % H2O2, Cleaning in place (CIP, 5 % NaOH, 90 °C, 194 °F) Acidic agents (HCl, H2SO4), max. 4 – 5 % one- or two-point calibration www.loligosystems.com 1201_Loligo-handout_21x21cm.indd 4 13/05/16 08:15
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