high through-put respiration assay for small aquatic

HIGH THROUGH-PUT RESPIRATION
ASSAY FOR SMALL AQUATIC ORGANISMS
Use this multi channel system for measuring oxygen consumption inside
80-1700 µL gas tight glass chambers for small aquatic organisms like individual
daphnia, zebrafish larvae, embryos, eggs etc.
Toldboden 3, 2nd floor · DK-8800 Viborg
Phone: +45 3360 2545
[email protected]
MICROPLATE WELL DIMENSIONS
ID
(mm)
DEPTH
(mm)
VOLUME
(µL)
4.5
5
80
6
7
200
500
8
10
10
12
940
12
15
1700
FEATURES
SYSTEM COMPONENTS
•Dual reference optical fluorescence O2sensing technology
•Real-time O2 monitoring in each independent chamber
•Non-invasive & non-destructive measurements
•Reusable oxygen sensor spots
•Experimental temperature range 5-40°C
•Connect 1-10 readers (24-240 chambers) to a single PC
•24-channel microplate oxygen reader
•24 oxygen sensor spots
•Glass microplate with 24 chambers
•Plastic caps with PTFE seals
•Water bath for temperature control
•Splitter and cable for PC communication
•Software for Windows
www.loligosystems.com
1201_Loligo-handout_21x21cm.indd 3
13/05/16 08:15
HIGH THROUGH-PUT RESPIRATION
ASSAY FOR SMALL AQUATIC ORGANISMS
Toldboden 3, 2nd floor · DK-8800 Viborg
Phone: +45 3360 2545
[email protected]
GLASS CHAMBER MICROPLATES
• Plates with 24 chambers
• Multiple chamber sizes
• Each chamber fitted with oxygen sensor spot
• Gas-tight chamber sealing
• Clean and sterilize with ethanol
• Reusable sensor spots
Specifications table for microplate reader system
Specifications
Compatible oxygen sensor
Oxygen channels
Power supply
Power adapter
Communication interface
Dimensions [mm]
Weight [g]
Windows version
Oxygen unit
Microplate reader system
Sensor spots
24
18-24 V DC
100-240V AC in; 18-24 V DC out
RS232
163 x 89 x 22
380
XP, Vista, WIN7, WIN10
% oxygen saturation, % air saturation, kPa, Torr, mg/L, mmol or ml/L.
0 – 50 % oxygen saturation
0 – 235 % air saturation
0 – 50 kPa
0 – 235 Torr
0 – 22,5 mg/L
0 – 700 mol
0 – 22,5 ml/L
± 0.4 % O2 at 20.9 % O2
± 4 hPa at 207 hPa
± 5 mol at 283.1 mol
± 2 % air saturation at 100 % air saturation
± 1 % O2 at 20.9 % O2
± 5 % air saturation at 100 % air saturation
< 0.2 % O2 within one week
< 1 % air saturation within one week
5 – 45 °C
< 30 sec.
Measurement range
Resolution
Precision
Drift at 0 % oxygen
(sampling interval 10 min)
Measurement temperature range
Response time (T90)
Sensor properties
Compatibility
Cross-sensitivity to
Cleaning procedures
Calibration
MINI
Aqueous solutions, ethanol, methanol
Reduced to ionic strength (salinity); high concentration of
small fluorescent molecules in the visible range can interfere
3 % H2O2, Cleaning in place (CIP, 5 % NaOH, 90 °C, 194 °F)
Acidic agents (HCl, H2SO4), max. 4 – 5 %
one- or two-point calibration
www.loligosystems.com
1201_Loligo-handout_21x21cm.indd 4
13/05/16 08:15