Supplemental Table S1. Cluster fresh weight, leaf area and leaf/cluster ratio of Cabernet
Sauvignon fruiting cuttings with different two, six, and twelve leaves per cluster. Asterisks
denote significant differences (*P<0.05)
Cluster weight (g)
Leaf area (m2)
Leaf/cluster ratio (m2/Kg)
2L
82,51 ± 8,18
0,0356 ± 0,0105
0,4363 ± 0,1425
6L
113,90 ± 15,78
0,0710 ± 0,0066
0,6375 ± 0,1554
12L
100,56 ± 17,66
0,1556 ± 0,0342*
1,6249 ± 0,6728*
Supplemental Table S2. Primers used for in real-time PCR studies.
Primer forward
(5' - 3')
Primer reverse
(5' - 3')
Accession number
(12x)
VvGPT1
TCAATGCTCTTGG
AGCTGCCATC
CTTCCTCACTGCTT
TGCCTGAG
GSVIVT00006900001
VvPGM
GATTCAGGCGCT
GTTCAAT
CCATTTCCTGCTGC
AATTT
GSVIVT01018452001
VvNTT
TTTCAGCAAGAGT
GCCAAGT
TCCCTTTAACCTTG
GTGTCC
GSVIVT01017211001
VvAGPB1
ACTTCCCTCGGAT
TGAGAGTGC
ACCAATGTCTTCCC
AGTAGCCATC
GSVIVT01030141001
VvAGPS1
GAGGCAGGAATG
AAATGGTT
TGCATCCTCAAATA
CCCAAA
GSVIVT01023805001
VvSS1
TCGGATACTGAC
AGTTAGCAAGGG
ATGCAGACCATAT
CCACCTTCGG
GSVIVT01038557001
VvSS2
TGCAAGGCAGCT
ATTGAGGTTCC
TCCCATCTCCATAG
CAGACACCAC
GSVIVT01011429001
VvSS3
CCTTAGAACGCA
ATGGACAGGTTG
AAGCAAAGTCGAG
CACGGTCA
GSVIVT01012723001
VvSS4
TGGCACCAGTTGC
TAAGGTTG
TGCAGTGCTCTGCT
AAGACCAG
GSVIVT01021404001
4
VvSS5
ACAAGGTACCCTT
GATCGTTTGC
CCAGAACAATTAT
CTGCCCACCAC
GSVIVT01032233001
VvGBSS1
GTGATGTTCTCGG
TGGTCTG
CCCAACTGGAATC
TCAACAA
GSVIVT01019680001
VVSuSy
(Hren et al. 2009)
VvSWEET1
TGTTAAGGCTCCT
GGATTTCAATTA
AGCCAAATCTTGG
CAAGCA
GSVIVT01015018001
TTCTTGGCTCCAC
TGTATGG
GCCCTCTCCTTCTT
GATTGA
GSVIVT01010015001
VvSWEET4
(Chong et al 2014)
VvSPS1
GGCTCGGACTGT
GATTGGTA
ACATGCAGTTCATC
ACTGTGG
GSVIVT01032489001
GGTTTGCCTATTG
TTGCCACCAG
CAAGAAGGCCATT
GTCAAGTACCC
GSVIVT01012825001
VvSUC11
(Pastenes et al., 2014)
VvSUC12
(Pastenes et al. 2014)
VvSUC27
(Pastenes et al. 2014)
VvSTS1
TGTGCCAATCTCA
AGTCTGCC
CCTGGGCTGCTGTT
ATGCTT
GSVIVT01009254001
ACCAGCCTCACC
ATTTATCAGAC
ATTTCATAACTGCT
CTCAGGGTTG
GSVIVT01020031001
TGCTTGGCACTGA
CGGTACT
GCTGTAGGTGATC
GCAAGAGG
GSVIVT01034886001
CGAAGCAACTAG
GCATGTGT
CTCCCCAATCCAAT
CCTTCA
GSVIVT01010590001
TTCCGTGTTCCTA
VvGAPDH
(Gainza-Cortés et al. CTGTTG
2012)
CCTCTGACTCCTCC
TTGAT
GSVIVT000097170
01
Supplemental Figure S1. Scheme of the starch biosynthesis pathway in grapevine woody
tissues. G6-Pi is transported into the amyloplast by the action of a GPT and converted into G1-Pi
by PGM. Following this reaction, G1-Pi and ATP are combined into ADP-gluc, by the action of
AGPase, and its glucosyl moiety is added to the starch granule by the action of starch synthases.
ADP-gluc, ADP-Glucose; AGPase, ADP-glucopyrophosphorylase; G1-Pi, glucose-1-phosphate;
G6-Pi, glucose-6-phosphate; GPT, glucose-6-phosphate/phosphate translocator; NTT, plastidic
nucleotide transporter; PGM, phosphoglucomutase; Pi, inorganic phosphate; PPi, inorganic
pyrophosphate.
Supplemental Figure S2. Phylogenetic tree of AGPase complex subunits. The tree was
constructed after aligning sequences from grapevine (Vitis vinifera), potato (Solanum tuberosum)
and Arabidopsis thaliana.
Supplemental Figure S3. Phylogenetic tree of starch synthases. The tree was constructed after
aligning sequences from grapevine (Vitis vinifera), potato (Solanum tuberosum) and Arabidopsis
thaliana.