CHEM 221 Elementary Biochemistry, Fall 2015
Lecture instructor: Dr. Jenq-Kuen (Jack) Huang
Office: Currens 548B
Office hours: M. W. F. 1:30-2:50 pm, other times by appointment.
Tel: 309-298-1207; Fax: 309-298-2180; email: [email protected]
Lecture meeting time and place: MWF 11:00-11:50 am. Currens 203
Laboratory meeting time and place: Th. 8:00-10:50 am; or 11:00-1:50 pm; or 3:005:50 pm, Currens 535
Course prerequisite: Chem 102 (3hrs lecture and 3hrs lab) or equivalent
Text: Biochemistry by Campbell, 7th edition 2011
Course objective: The course is intended to teach the structure, properties, function
and metabolism of biological molecules with emphasis on macromolecules.
Tests and Grading scale:
This course consists of three credits of lecture work and one credit of laboratory.
Therefore the semester grade will consist of 75% (600 points) from the lecture and 25%
(200 points) from the lab. In order to receive credit for the course as a whole, one must
complete the lab with at least 60%. Failure to complete the lab will result in failure of the
course. There are total 800 possible points. Your letter grade will be based on your total
accumulated points.
Course Grading Scale:
90.00% and above
86.70 - 89.99%
83.40 - 86.69%
80.00 - 83.39%
76.70 - 79.99%
73.40 - 76.69%
70.00 - 73.39%
66.70 - 69.99%
63.40 - 66.69%
60.00 - 63.39%
56.70 - 59.99%
56.66% and below
Lecture grade computation:
3 exams
Best 8 of 10 quizzes
4 Homework assignments
Comprehensive final
Total
A
AB+
B
BC+
C
CD+
D
DF
300 points
80 points
120 points
100 points
600 points
Exams and Quizzes: Three 50-min exams will be given during the semester. Exams
and Short quizzes (use the first 10 minutes of the class time) will be given on
Wednesdays. Any Student who is tardy loses that amount of time to work on the
exam or quiz. No makeup quiz or exam will be allowed unless you have acceptable
excuses such as personal illness or family emergencies or participate in University
sponsored functions (i.e. WIU band trips, field trips in other WIU classes, professional
meeting (not sorority/frat, or other clubs), WIU athletics, etc.). In all cases,
documentation is required and must be submitted to the instructor in a week prior to the
event or as soon as possible in case of emergency. Makeup quiz or exam must be
completed in the same week. It is the responsibility of the student to schedule the
makeup quiz or exam with the instructor in a timely manner.
An incomplete grade will NOT be given to a student with a failing grade. No incomplete
will be given to a student without documented evidence of an emergency that requires
the student be away from the university or miss the final examination. The student shall
notify the instructor of the emergency as soon as possible and prior to the final
examination.
Seating will be assigned by the instructor during the exams and/or any other time
deemed necessary. Simple scientific calculators may be used on quizzes and exams.
Please silent cell phones and put away laptops while in class. Cell phones and laptops
can be very distracting and can’t be out during quizzes and exams. Also, class time is
not a social hour; please refrain from casual conversation during class time.
Attendance: Regular and punctual class attendance is vitally important to a student's
academic achievement. You are expected to attend class regularly and punctually. All
students are responsible for all information and materials given in class whether you are
present or not. Attendance at the lecture will be checked daily; attendance at the
laboratory is required. Excessive absences will be reported to the financial aid office.
Outside Work Requirements: Students are expected to read ahead and be prepared
for class. You will probably find that this is a very fast-paced course. Therefore, it is
highly advisable to keep up with the materials. Past experience suggests that reading
the designated chapter before the class and going over the notes after each class is
essential. A daily minimum of two hours of out of class work is needed to do well in this
class. Write down any questions at that point. You may submit your questions by email
or ask in the next lecture period or visit Chemistry Resource Center or your instructor
during office hours.
Chemistry Resource Center: Chemistry Resource Center is located in Currens 107.
Free tutoring and/or help is provided by the department through the Center. Hours will
be posted.
Statement on Ethics: Western Illinois University, like all communities, functions best
when its members treat one another with honesty, fairness, respect, and trust. Students
have rights and responsibilities (http://www.wiu.edu/provost/students/). Plagiarism,
cheating, and other forms of academic dishonesty constitute a serious violation of
University conduct regulations. Students who engage in dishonesty in any form shall be
charged with academic dishonesty. Please remember that you are expected to do
your own work at all times.
Emergency evacuation procedures for the building: If a fire alarm should happen to
ring, or if students are ordered to evacuate a lab or classroom by the instructor, the
students should walk to the nearest stairwell (Do not use the elevators) and proceed to
the ground floor and out the building. Any student on an upper floor who cannot
physically proceed down the stairs should go to the southernmost stairwell and await
assistance. If the building should be evacuated all students and personnel should
gather at the southwest corner of the Higgins Parking lot near the fence (parking lot just
outside the building) to wait further instructions.
Emergency Preparedness: WIU Office of Risk Management and Emergency
Preparednessprovides resources on how to respond to emergency situations. Please
view the video resources at www.wiu.edu/rmep/ (Click “Resources” on the left side of
the page).
Students with Disabilities: In accordance with University values and disability law,
students with disabilities may request academic accommodations where there are
aspects of a course that result in barriers to inclusion or accurate assessment of
achievement. To file an official request for disability-related accommodations, please
contact the Disability Resource Center at 309-298-2512, [email protected] or in 143
Memorial Hall. Please notify the instructor as soon as possible to ensure that this
course is accessible to you in a timely manner.
The following action is prohibited under the Student Conduct Code:
Disorderly Conduct: Any behavior which disrupts the regular or normal functions of the
University community, including behavior which breaches the peace or violates the
rights of others. (http://sjp.wiu.edu/CodeOfConduct/index.asp)
STATE ACCREDITATION POLICY INFORMATION:
“In accordance with Illinois State Board of Education certification
rules, all candidates seeking teacher certification are required by
Western Illinois University to obtain a grade of “C” or better in all
directed general education course, all core courses, and all courses in
the option. Note: A “C- ” is below a “C”.” Please note: any secondary
science teacher certification student wanting to see how this course is
aligned with the State and National Standards should see their advisor
and/or examine the Secondary Science Teacher Certification
WesternOnline Advising site.
Tentative Lecture/Exam Schedule:
Chap 1. Biochemistry and the organization of cells
Chap 2. Water: The Solvent for Biochemical Reactions
Chap 3. Amino Acids and Peptides
Chap 4 The Three-Dimensional Structure of Proteins
Tuesday, Sept. 22 by noon ---- first homework due
Wednesday, Sept. 23 ---- first 50-min exam
Chap 5. Protein Purification and Characterization Techniques
Chap 6. The Behavior of Proteins: Enzymes
Chap 7. The Behavior of Proteins: Enzymes, Mechanisms, and Control
Chap 8. Lipids and Proteins Are Associated in Biological Membranes
Tuesday, Oct. 20 by noon ---- second homework due
Wednesday, Oct. 21 ----- second 50-min exam
Chap 9. Nucleic Acids: How Structure Conveys Information
Chap 10. Biosynthesis of Nucleic Acids: Replication
Chap 11. Transcription of the Genetic Code: The Biosynthesis of RNA
Chap 16. Carbohydrates
Tuesday, Nov.17 by noon ---- third homework due
Wednesday, Nov. 18 ---- third 50-min exam
Chap 12. Protein Synthesis: Translation of the Genetic Message
Chap 13. Nucleic Acid Biotechnology Techniques
Chap 17. Glycolysis
Chaps .19 & 20 The Citric Acid Cycle & Electron Transport and Oxidative
Phosphorylation
Friday, Dec. 11 by noon ---- fourth homework due
Wednesday, Dec. 16, 10:00 am ----------- final exam
Holidays and Other Important Dates:
Date
Event
August 24, Mon. 2015
First day of classes
September 7, Mon. 2015
Labor day holiday
October 16, Fri. 2015
Fall break
November 23-27, Mon. -Fri. 2015 Thanksgiving break
December 14-18, Mon. -Fri. 2015 Final exam week
December 19, Sat. 2015
Commencement in Macomb
SYLLABUS MAY BE SUBJECTED TO CHANGE WITH NOTICE.
Last Updated Aug. 8, 2015 by Dr. Jenq-Kuen (Jack) Huang
CHEM 221 Laboratory (Fall 2015)
Laboratory coordinator: Dr. Jenq-Kuen (Jack) Huang, Currens 548B; office Hours: M.
W. F. 1:30-2:50 pm; other times by appointment.
Laboratory meeting time and lab instructor:
Meeting time
Lab instructor
Section 21
Thursday. 8:00-10:50 am Mr. Michael Bordowitz
Section 22
Thursday. 11:00-1:50 pm Mr. Michael Bordowitz
Section 23
Thursday. 3:00-5:50 pm
Mr. Praveen Yeredla
Laboratory meeting place: Currens 535
General: Safety is the first concern in a chemistry lab. Material Safety Data Sheets
(MSDS) information about all chemicals utilized in the laboratory can be found at the
following web site. http://hazard.com/msds/. Proper handling of chemicals, especially
correct pipetting technique is expected. Safety glasses are to be worn at all times.
Students without goggles, closed-toe shoes or proper attire (Pants must cover the entire
shin. Shirts must cover the entire shoulder) will not be admitted to the lab, and this will
count as a missed lab session.
Students enrolled in this course are levied a non-refundable laboratory usage fee of $35
to cover the cost of consumable supplies utilized during the semester.
Grade determination:
Lab reports (10 reports)
Pre lab quizzes (10 quizzes)
Midterm
Lab final
Attend lectures during lab time
Total
Points
140
20
15
20
5
200
The TAs will collaborate so as to grade each lab section in a uniform manner. Overall,
the lab will count as 200 points and it will be added to your lecture portion points to get a
final grade for this course. Failure to complete the lab with at least 60% (>120
points) will result in failure of the course.
Pre-lab quizzes: A pre-lab quiz will be given during the initial 5 minutes of the lab
period each week. A student who is tardy loses that amount of time to work on the quiz.
A student who is 5 or more minutes late will not be permitted to take the quiz and will
receive a grade of zero.
Pre and post lab reports: The reports are to be typed. However calculation and
equations may be written legibly by hand. The pre-lab section of the report is to be
completed before coming to lab. Your instructor will check your pre-lab report for each
laboratory period. Failure to complete the pre-lab section of the lab report before the
start of the lab will result in losing 6 points for each report. Although much of the lab
work will be done in pairs, the lab reports should represent the individual student's work.
Plagiarism will NOT be tolerated. Incidents of plagiarism will results in a grade of zero
for the assignment and failing grade for the whole course.
Pre-lab report must include the following:
 Cover page- a stand-alone cover page must include experimental number and
title, course number, date the experiment was done, your name (in bold) and
your lab partner’s name, TA and instructor’s names. (0.5 point)
 Objective(s)- state one or two sentences the objectives of the experiment (0.5
point)
 Theory/Background (pertinent background information) (2 point)
o Write a paragraph (should not less than half page) to summarize the
basics of how the objectives will be realized. This may include important
reactions, techniques or instrumental methods. It should not include
procedures.
 Materials and Methods (3 point)
o Materials- list all reagents and materials used in the experiment including
the concentrations of solutions
o Procedures- the detail should be sufficient so that your fellow students
can read your report without guessing. Any changes of procedures or
concentration of solutions during lab sessions must be recorded.
Post-lab report: Data you take in the laboratory are to be recorded in your notebook as
you take them. It is recommended that you complete the post-lab data analysis during
the lab period immediately after the completion of the procedures. Post-lab report must
include the following:
o Results (6 points)
o These should include tables, graphs and calculations, if any
o Tables and graphs should have self-explanatory titles
o Numbers should have proper units
o X axis and Y axis should be labeled
o All calculations should be included with appropriate units, if any
o Discussions/Conclusions (2 point)
o Relate results to objective and theory.
o Answer questions posed in the procedure, if any.
o Explain unexpected results or deviations from the written procedure
Both pre-lab and post-lab sections of the report must be submitted together at the
beginning of the next lab period. Late lab reports will be docked by 5% for each day.
Lab reports turned in two weeks after the due date will not be graded. Although much of
the lab work will be done in pairs, the lab reports should represent the individual
student's work.
Attendance: Attendance in the laboratory is required. Should it be necessary to miss a
lab, please notify your instructor in writing, before the fact if possible. Only one make-up
lab is allowed at the end of the semester. The only acceptable excuses for missing a lab
are personal illness or family emergencies or participation in University sponsored trips.
In any case, documentations are required.
Cell phones are NOT to be used during the lab.
Tentative lab exercises
Date
Expt #
Activity
Points
Quiz/report
8/27
Dr. Huang will use two hours lab time to deliver lecture
in Currens 315
2.5
9/3
Dr. Huang will use two hours lab time to deliver lecture
in Currens 315
2.5
9/10
1
Go over syllabus; acid-base titration
Please watch the videos before coming to the lab:
- http://ocw.mit.edu/resources/res-5-0001-digital-labtechniques-manual-spring-2007/videos/volumetrictechniques/
- http://ocw.mit.edu/resources/res-5-0001-digital-labtechniques-manual-spring-2007/videos/titration
2/14
9/17
2
Buffer
2/14
9/24
3
Methyl red colorimetry
https://www.youtube.com/watch?v=QdufRwbkeKo
2/14
10/1
4
Learn to use pipetman
http://ocw.mit.edu/resources/res-5-0001-digital-labtechniques-manual-spring-2007/videos/using-anautomatic-pipet/
2/14
10/8
5
Quantitative protein color reactions
2/14
10/15
6
Survey of protein diversity
2/14
Midterm exam
15
10/22
10/29
7
Size exclusion chromatography
2/14
11/5
8
Plasmid isolation and agarose gel electrophoresis
http://www.youtube.com/watch?v=8xEDEJ0DHFA
https://www.youtube.com/watch?v=eN_cw65oQZQ
2/14
11/12
9
Reducing sugar reaction of carbohydrate
2/14
11/19
10
Paper chromatography of carbohydrates
2/14
11/26
No lab (Thanksgiving break)
12/3
Make up lab (allowed only one made up lab, must sign
up a week in advance. Experiment to be determined)
--
12/10
Comprehensive lab final
20
Supplies to be provided by students:
o Safety goggles
o Scientific Calculator
o Notebook
SYLLABUS MAY BE SUBJECTED TO CHANGE WITH NOTICE.
Last Updated: Aug. 22, 2015 by Dr. Jenq-Kuen (Jack) Huang
Department of Chemistry, Western Illinois University