Effect of hydrogel on the turf grass species growing under drought stress Anna Hadam As the supply of urban lawn grass‘ irrigation is expensive and laborious it is important to find other methods which are effective against the water deficiency. The aim of the study was therefore the assessment of hydrogels‘ influence on the the most common grass species (Festuca rubra, Festuca ovina, Lollium perenne) which were cultivated under controlled drought stress. Results indicated that this watersorbent increased the germination, growth and dry matter content of tested species. It was therefore concluded that using hydrogels on grasslands seems to be reasonable in accordance to the water deficiency problems. Key words: watersorbent, drought, Festuca rubra, Festuca ovina, Lollium perenne. Introduction Lawn grasses are the most common green areas near the road sides which play an important role in landfill landscaping and shaping the microclimate conditions. Unfortuantely many stresses, espesially drought, decrease their growth and condition what causes that some special cultivation is needed. As traditional irrigation is expensive and laborious other methods, effective against the water deficiency, have to be found. One of the solution may be using the watersorbents, called hydrogels. Since these substances are able to absorb the huge amount of water, they increase the watercapicity of soil, decrease evapotransporation and allow to mitigate the drought stress. The aim of the study was the preliminary assessment of grassess‘ germination, growth and dry matter content under drought stress conditions with and without hydrogel’s additive. Conducted experiment was part of the longterm studies assessing effectiveness of hydrogels under several stressess, common in the near roads areas. Materials and methods The study was conducted in a greenhouse of the Warsaw University of the Life Science, from June to October 2009. Three grass species: Festuca rubra, Festuca ovina and Lollium perenne were grown in pots (12 cm diameter) containing sand, peat and garden mould (in proportion 1:1:1). To half of the pots the Hydrogel „BASF“ was added, in the amount required by the manufacturer (2g /L of soil). Number of seeds planted per pot dependend on the: seedling emergence and seedling purity of each grass species [1]. There were at least 72 pots, with 24 pots assigned to each species. At the begining of the study all seedlings were fully watered. Only after 5 days the plants were subjected to the three levels of the water regime, differing in the frequence of watering (per 2, 7 and 14 days), but the amount of water was everywhere the same (75 mL/ pot). Each regime was replicated three times. During experiment grass were mowing once a week to 5 cm lenght. The temperature and air humidity in the greenhouse were measured everyday by the termohygrometer EPI 8703. Effectiveness of hydrogel was assessed on the basis of the grass species reactions such as: germination index, turfs height and dry matter content. Results of three replications were subjected to Duncan‘s test for the analysis of variance (ANOVA) with statistical significance set at p = 0.05. Natural Sciences Department of Environmental Protection, University of the Life Science in Warsaw, 0-608-072-333, e-mail: [email protected] 3 Anna Hadam Results and disscussion It is commonly known that drought can decrease the germintaion of seedlings [2–4]. Mangol and Sheley [5] reported however, that additive of hydrogel increases the seedlings emergence of wheatgrass, similar results were given also for lettuce, trifolium and reygrass [6]. Results, presented in this paper, indicated that, influence of hydrogel on germination index was depended on the tested species. The best germination of Festuca rubra was observed in pots with hydrogel watered optimally (per 2 days), but with increasing of the drought period, number of ger- minated seeds decreased (Fig. 1). Nevertheless even during 7-days water deficiency, number of germinated seeds in pots with hydrogel was still significant greater than in pots without hydrogel. Only with 14-days drought, germination of Festuca rubra did not dependent on hydrogels’ additive — number of seeds germinated in pots with hydrogel were comparable with those in a control pots. Seeds of Festuca ovina germinated independently on a hydrogel presence. In both pots with and without hydrogel number of germinated seeds significantly decreased with duration of the drought stress (Fig. 2). Hydrogel increased however the germination of Lollium perenne. Only when watering was optimal (per 2-days) number of seedlings in pots with hydrogel were comparable with a control, but under drought stress (7-days and 14days period) germination index in pots with hydrogel was significantly greater than in control (Fig. 3). Above differences in results between each species may be associated with their genetic responce to avoid drought Figs. 1–3. Influence of hydrogel on germination of Festuca rubra, Festuca ovina and Lollium perenne. Figs. 4–6. Influence of hydrogel on early growth of Festuca rubra, Festuca ovina and Lollium perenne. Culture conditions During all experiment the temperature was maintained between 24.9 and 29.0°C, while the air humidity was ranged from 32.5 to 45.0%. Effect on germination 4 Effect of hydrogel on the turf grass species growing under drought stress [7, 8], Festuca rubra especially and Lollium perenne are known as the most resistance turf grasses in acccordance to the water deficiency [9]. Effect on the turfs hight Effect on the dry matter content In compare to the control, hydrogel significantly increased the dry matter content of: all species watered Natural Sciences A number of reports on plants’ reaction on the drought stress show, that there are several common responces in their early growth [10–12]. One of the first of them is the decreasing of the shoot lenght [13], but hydrogel is known to inhibit this decreasing [14]. Nevertheless in the early stage (26th of June) hydrogel influenced on Festuca rubra only (Fig. 4). Until 7-days drought was conducted seedlings of this species cultivated with hydrogel were significantly higher than in a control pots even those watered per 2 days. Only 14-days water deficiency significantly decreased growth of F. rubra in all pots — with and without hydrogel. It can be therefore seen that not only hydrogel additive but also species influenced again on the drought stress reaction. Hydrogel did not affected the early growth of Festuca ovina and Lollium perenne. Turfs height of these species decreased with the period of a drought stress, as well in control pots as in pots with the hydrogel (Figs. 5 and 6). It was however observed that, the longer experiment was conducted, the influence of hydrogel on grasses was getting stronger (Figs. 7–9.). Despite there was no significant difference in case of pots watered optimally, during 7-days drought hydrogel increased growth of all species. It was also observed that in compare with a control pots F. rubra seedlings growed also better with hydrogel during 14-days stress. At the end of the study (1st of October) hydrogel increased turfs height of all species and in all water conditions (Figs. 10–12). Figs. 7–9. Influence of hydrogel on growth of Festuca rubra, Festuca ovina and Lollium perenne. Figs. 10–12. Influence of hydrogel on growth of Festuca rubra, Festuca ovina and Lollium perenne. 5 Anna Hadam optimally or exposed to 7-days drought (Figs. 13–15) and F. rubra during 14-days drought period. Similar results were reported in the studies on hydrogel and turf grassess conducted by Breś [15], who suggested that this phenomena can be associated with the nutirent ingriedients in hydrogels. would be therefore useful to study also the varieties of each species. It can be preliminary concluded that using hydrogels on grasslands seems to be reasonable in accordance to the water deficiency problems. Nevertheless to confirm it’s real effectiveness also the field study are needed. References Figs. 13–15. Influence of hydrogel on dry matter of Festuca rubra, Festuca ovina and Lollium perenne. Conclusions Hydrogel „BASF“ influenced postively on germination, growth and dry matter content of tested grass species cultivated under controlled drought stress. 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