No.TI449E
Coenzymes (Nicotinamide adenine dinucleotides)
1
2
H2 N
+N
NH2
OH
HO
O
O
N
N
O
C
N
N
O
CH2O P O P OCH2
OOH
OH
HO
O
beta-nicotinamide adenine dinucleotide
oxidized form
(NAD)
1
3
O
2
NH2
OH
HO
N
O
H2 N
N
N
O
CH2O P O P OCH2
ONa
mV
100
N
N
O
ONa
C
OH
HO
O
beta-nicotinamide adenine dinucleotide
reduced form, disodium
(NADH)
4
3
H2 N
+N
NH2
OH
HO
O
O
N
N
O
C
N
N
O
CH2O P O P OCH2
OOH
O
HO
O
HO P O
ONa
0
0
2
4
min
Unison UK-Amino, 30 x 3 mm
A: acetonitrile
B: water /triethylamine /acetic acid
= 100 /4.1 /1.6
20-85%B (0-5min), 0.6 mL/min (2MPa)
50 deg.C, 260 nm, 0.4 uL (0.5-1.3ug)
beta-nicotinamide adenine dinucleotide
phosphate,oxidized form, sodium
(NADP)
O
4
N
NH2
OH
HO
O
C
O
N
O
CH2O P O P OCH2
ONa
H2 N
N
N
O
N
ONa
HO
O
NaO P O
ONa
beta-nicotinamide adenine dinucleotide
phosphate,reduced form, tetrasodium
(NADPH)
TI449E-HK10