Functional Analysis and Transcriptomic Profiling of iPSCDerived Macrophages and Their Application in Modeling
Mendelian DiseaseNovelty and Significance
by Hanrui Zhang, Chenyi Xue, Rhia Shah, Kate Bermingham, Christine C. Hinkle,
Wenjun Li, Amrith Rodrigues, Jennifer Tabita-Martinez, John S. Millar, Marina
Cuchel, Evanthia E. Pashos, Ying Liu, Ruilan Yan, Wenli Yang, Sager J. Gosai,
Daniel VanDorn, Stella T. Chou, Brian D. Gregory, Edward E. Morrisey, Mingyao Li,
Daniel J. Rader, and Muredach P. Reilly
Circulation Research
Volume 117(1):17-28
June 19, 2015
Copyright © American Heart Association, Inc. All rights reserved.
Schematic figure of induced pluripotent stem cell (iPSC)–derived macrophages (IPSDM)
differentiation.
Hanrui Zhang et al. Circ Res. 2015;117:17-28
Copyright © American Heart Association, Inc. All rights reserved.
Induced pluripotent stem cell–derived macrophages (IPSDMs) are comparable with human
peripheral blood mononuclear cell–derived macrophages (HMDMs) at morphological and
immunophenotypic levels.
Hanrui Zhang et al. Circ Res. 2015;117:17-28
Copyright © American Heart Association, Inc. All rights reserved.
Functional phenotypes in response to M1 (lipopolysaccharide [LPS]+interferon [IFN]-γ) and M2
(interleukin [IL]-4) polarization are comparable between induced pluripotent stem cell–derived
macrophages (IPSDMs) and human peripheral blood mononuclear cell–derived macrophages
(HMDMs).
Hanrui Zhang et al. Circ Res. 2015;117:17-28
Copyright © American Heart Association, Inc. All rights reserved.
RNA-Seq transcriptome analysis of induced pluripotent stem cells (iPSCs), iPSC-derived
macrophages (IPSDMs) and human peripheral blood mononuclear cell–derived macrophages
(HMDMs).
Hanrui Zhang et al. Circ Res. 2015;117:17-28
Copyright © American Heart Association, Inc. All rights reserved.
Induced pluripotent stem cell–derived macrophage (IPSDM) recapitulates transcriptome
signature of M1 (lipopolysaccharide [LPS]+interferon [IFN]-γ) and M2 (interleukin [IL]-4)
macrophage polarization.
Hanrui Zhang et al. Circ Res. 2015;117:17-28
Copyright © American Heart Association, Inc. All rights reserved.
Tangier disease (TD)-induced pluripotent stem cell–derived macrophages (IPSDMs) recapitulate
hallmark phenotypes of impaired cholesterol efflux and reveal novel inflammatory phenotype in
TD-IPSDM.
Hanrui Zhang et al. Circ Res. 2015;117:17-28
Copyright © American Heart Association, Inc. All rights reserved.