Epub: No 2015_1235
Vol. 63, 2016
http://dx.doi.org/10.18388/abp.2015_1235
Review
Effect of different wavelengths of light on laccase, cellobiose
dehydrogenase, and proteases produced by Cerrena unicolor,
Pycnoporus sanguineus and Phlebia lindtneri
Grzegorz Janusz, Justyna Sulej, Magdalena Jaszek and Monika Osińska-Jaroszuk
Department of Biochemistry, Maria Curie-Skłodowska University, Lublin, Poland
Three species of white rot fungi: Cerrena unicolor, Phlebia lindtneri and Pycnoporus sanguineus were cultured
in two different media under five different lighting conditions: dark, white, red, blue, and green light. Laccase,
cellobiose dehydrogenase, and protease activities were
examined in the samples. Blue light efficiently boosted
laccase synthesis in C. unicolor and P. sanguineus, whereas the highest activities (20 654 nkat/l) of P. lindtneri laccase were observed when this fungus was maintained
in green light. On the contrary, the green light allowed
obtaining the highest activities of cellobiose dehydrogenase of C. unicolor and P. lindtneri, while CDH of P. sanguineus seems to be dependent on white light. It is
clearly visible that differences in protease activities are
noticeable not only between the lights variants but also
among the media used. However, high proteases activities are correlated with light variants inducing laccase in
Lindeberg and Holm medium. Contrary to the cellulosebased medium, where they are weak in light variants
that lead to high CDH activities.
Key words: white rot fungi, submerged cultures, visible light wavelenghts
Received: 07 January, 2016; revised: 08 January, 2016; accepted:
11 January, 2016; available on-line: 17 February, 2016
INTRODUCTION
During millions years of evolution, light became a crucial factor for most living organisms in terms of both the
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e-mail: [email protected]
Abbreviations: FCL, Fungal Culture Collection of Lublin; DCIP,
2,6-dichloroindophenol; CDH, cellobiose dehydrogenase; WWC,
white collar complex
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VSHFLHV Cerrena unicolor, Pycnoporus sanguineus DQG Phlebia
lindtneri
MATERIAL AND METHODS
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RESULTS
Effect of different light wavelengths on laccase
activities
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Vol. 63
Light influence on fungal metabolism
3
Figure 1. C. unicolor grown in Lindeberg and Holm medium under white, green, blue, red lights and in the dark.
(A) laccase activity. Proteolytic activities (B) were analyzed by gelatin zymography of the extracellular media. 6 μl of samples (5 µg
of protein) were loaded on a polyacrylamide gel containing 0.3%
of gelatin. After electrophoresis, proteins were renaturated in the
gel and the proteolytic activity was revealed after a 16-h incubation required for gelatin degradation
Figure 2. P. sanguineus grown in Lindeberg and Holm medium
under white, green, blue, red lights and in the dark.
(A) laccase activity. Proteolytic activities (B) were analyzed by gelatin zymography of the extracellular media. 6 μl of samples (5 µg
of protein) were loaded on a polyacrylamide gel containing 0.3%
of gelatin. After electrophoresis, proteins were renaturated in the
gel and the proteolytic activity was revealed after a 16-h incubation required for gelatin degradation
tures of C. unicolor )LJ DQG P. lindtneri )LJ ZKHUH
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Figure 3. P. lindtneri grown in Lindeberg and Holm medium under white, green, blue, red lights and in the dark.
(A) laccase activity. Proteolytic activities (B) were analyzed by gelatin zymography of the extracellular media. 6 μl of samples (5 µg
of protein) were loaded on a polyacrylamide gel containing 0.3%
of gelatin. After electrophoresis, proteins were renaturated in the
gel and the proteolytic activity was revealed after a 16-h incubation required for gelatin degradation
Figure 4. C. unicolor grown in cellulose-based medium under
white, green, blue, red lights and in the dark.
(A) CDH activity. Proteolytic activities (B) were analyzed by gelatin
zymography of the extracellular media. 6 μl of samples (5 µg of
protein) were loaded on a polyacrylamide gel containing 0.3% of
gelatin. After electrophoresis, proteins were renaturated in the gel
and the proteolytic activity was revealed after a 16-h incubation
required for gelatin degradation
4
G. Janusz and others
2016
Figure 5. P. lindtneri grown in cellulose-based medium under
white, green, blue, red lights and in the dark.
(A) CDH activity. Proteolytic activities (B) were analyzed by gelatin
zymography of the extracellular media. 6 μl of samples (5 µg of
protein) were loaded on a polyacrylamide gel containing 0.3% of
gelatin. After electrophoresis, proteins were renaturated in the gel
and the proteolytic activity was revealed after a 16-h incubation
required for gelatin degradation
Figure 6. P. sanguineus grown in cellulose-based medium under
white, green, blue, red lights and in the dark.
(A) CDH activity. Proteolytic activities (B) were analyzed by gelatin
zymography of the extracellular media. 6 μl of samples (5 µg of
protein) were loaded on a polyacrylamide gel containing 0.3% of
gelatin. After electrophoresis, proteins were renaturated in the gel
and the proteolytic activity was revealed after a 16-h incubation
required for gelatin degradation.
PRVW WZRIROG C. unicolor DQG RYHU VL[IROG P. lindtneri)
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DISCUSSION
Protease activities dependence on light
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