Investigating Photosynthesis Demonstrating the conditions needed for photosynthesis to occur. Normal, in a light window In the dark Without chlorophyll Without carbon dioxide Leave Normal, in a light window plants Without chlorophyll in these conditions for a few days. In the dark Without carbon dioxide Now test for starch in the leaves. If there is starch in the leaf, then photosynthesis must have happened. If there is no starch in the leaf, then there was no photosynthesis Testing Leaves for Starch Place leaf in alcohol Testing Leaves for Starch Leave in hot water for 3 mins Testing Leaves for Starch Rinse in the hot water Testing Leaves for Starch Add 1 drop of iodine Rinse in the hot water Lay on a white tile Testing Leaves for Starch 5 Add 1 drop of iodine 1 Place leaf in alcohol 2 Leave in hot water for 3 minutes 3 Rinse in the hot water 4 Lay on a white tile Results of the Starch Test No Chlorophyll Normal No light No CO2 Starch present No starch No starch No starch in the yellow bits Photosynthesis occurred No photosynthesis without light No photosynthesis without carbon dioxide No photosynthesis without chlorophyll Conclusions For photosynthesis you must have: carbon dioxide water chlorophyll light
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