Type I interferons produced by dendritic cells promote
their phenotypic and functional activation
by Maria Montoya, Giovanna Schiavoni, Fabrizio Mattei, Ion Gresser, Filippo
Belardelli, Persephone Borrow, and David F. Tough
Blood
Volume 99(9):3263-3271
May 1, 2002
©2002 by American Society of Hematology
Phenotype of BM-DCs unable to respond to type I IFN.BM-DCs unable to respond to type I IFN
have a less activated phenotype than control BM-DCs.
Maria Montoya et al. Blood 2002;99:3263-3271
©2002 by American Society of Hematology
Expression of IFN-α and IFN-β mRNA in sDCs and BM-DCs.(A) BM-DCs.
Maria Montoya et al. Blood 2002;99:3263-3271
©2002 by American Society of Hematology
Ability of BM-DCs from type I IFN-R KO mice to stimulate CD8+ and CD4+ T-cell proliferation.BMDCs from type I IFN-R KO mice exhibit a reduced ability to stimulate CD8+ and CD4+ T-cell
proliferation.
Maria Montoya et al. Blood 2002;99:3263-3271
©2002 by American Society of Hematology
Activation of sDCs upon exposure to type I IFN in vivo or in vitro.(A) Expression of CD40, CD86,
or H-2Db on sDCs purified from B6 mice 4 hours after injection of PBS (filled histograms) or IFNα/β (open histograms, solid line).
Maria Montoya et al. Blood 2002;99:3263-3271
©2002 by American Society of Hematology
Expression of IFN-α by sDCs.The sDCs were purified from B6 mice, incubated for 1 hour in
medium containing GolgiPlug, placed on microscope slides, and fixed.
Maria Montoya et al. Blood 2002;99:3263-3271
©2002 by American Society of Hematology
Effect of overnight culture of sDCs.Overnight culture of sDCs induces phenotypic activation that
is partially dependent on type I IFN. (A) Phenotype of CD11c+sDCs from B6 mice directly ex vivo
(solid lines) and after overnight culture (dark-gray filled hist...
Maria Montoya et al. Blood 2002;99:3263-3271
©2002 by American Society of Hematology
Role of type I IFN in IFN-γ secretion by sDCs.Secretion of IFN-γ by sDCs is partially dependent
on type I IFN. Purified sDCs (greater than 97% CD11c+) were cultured in either medium alone,
medium plus 1000 neutralizing units of anti–IFN-α/β sheep immunoglob...
Maria Montoya et al. Blood 2002;99:3263-3271
©2002 by American Society of Hematology
Effect of IFN-γ treatment on the inhibitory effects of anti–IFN-α/β.Treatment with IFN-γ reverses
the inhibitory effects of anti–IFN-α/β on the phenotypic activation of sDCs.
Maria Montoya et al. Blood 2002;99:3263-3271
©2002 by American Society of Hematology
Reduced ability of sDCs to stimulate T-cell proliferation when cultured with anti–IFN-α/β.The
sDCs (96% CD11c+) isolated from B6 mice were cultured for 18 hours in the presence (circles,
dotted lines) or absence (squares, solid lines) of anti–IFN-α/β immuno...
Maria Montoya et al. Blood 2002;99:3263-3271
©2002 by American Society of Hematology