Aii
Ai
Bi
Aiii
Bii
Ci
Biii
Cii
Ciii
Supplementary Methods Figure 2. Identification of epithelial cells and tight junction protein expression. Ai. Forward scatter
versus Side scatter plot of mixed epithelial and lamina propria cells within live cell gate. Aii. Single parameter histogram
demonstrating region of CD3+ staining cells. Aiii. CD3+ staining back gated on light scatter histogram. Bi and Bii. Histograms
demonstrating positive region of PCK positive staining in reference to isotype control antibody. A region was drawn that
excludes irrelevant isotype-matched control antibody and nonspecific staining and all events exceeding isotype control staining
intensity were regarded as positively labeled epithelial cells. Biii. PCK + staining back gated on light scatter histogram.Ci.
Forward scatter versus light scatter plot of isolated epithlial cells with region drawn to exclude CD3+ contamination (percentage
expression of CD3+ cells= 0.4%; n=3). Cii and Ciii. Single parameter histograms representative of isotype control and epithelial
positive staining of tight junction protein antibody (here ZO-1) using super-enhanced Dmax (SED) normalised subtraction.