Identification of a 14-3-3 Binding Sequence in the Common
β Chain of the Granulocyte-Macrophage ColonyStimulating Factor (GM-CSF), Interleukin-3 (IL-3), and IL-5
Receptors That Is Serine-Phosphorylated by GM-CSF
by F.C. Stomski, M. Dottore, W. Winnall, M.A. Guthridge, J. Woodcock, C.J. Bagley,
D.T. Thomas, R.K. Andrews, M.C. Berndt, and A.F. Lopez
Blood
Volume 94(6):1933-1942
September 15, 1999
©1999 by American Society of Hematology
Schematic representation of the cytoplasmic domain of βc showing the overall sequence
identity between human and mouse βc and the seventh region of highest identity.
F.C. Stomski et al. Blood 1999;94:1933-1942
©1999 by American Society of Hematology
Comparison of sequences in signaling molecules known to bind 14-3-3ζ and the derived
consensus 14-3-3 binding motif.
F.C. Stomski et al. Blood 1999;94:1933-1942
©1999 by American Society of Hematology
Human βc associates with 14-3-3ζ, and this association is mediated by the 544-626 region of βc.
F.C. Stomski et al. Blood 1999;94:1933-1942
©1999 by American Society of Hematology
14-3-3ζ specifically binds the HSRSLP motif of the βc cytoplasmic domain.
F.C. Stomski et al. Blood 1999;94:1933-1942
©1999 by American Society of Hematology
Inhibition of βc association with 14-3-3ζ by phosphorylated but not by unphosphorylated βc and
raf-1 peptides.
F.C. Stomski et al. Blood 1999;94:1933-1942
©1999 by American Society of Hematology
Specific inhibition of βc association with 14-3-3ζ by a phosphorylated peptide encompassing the
579-592 region of βc.
F.C. Stomski et al. Blood 1999;94:1933-1942
©1999 by American Society of Hematology
Binding of 125I-labeled 14-3-3ζ to synthetic peptides corresponding to the 14-3-3 binding region
of βc.
F.C. Stomski et al. Blood 1999;94:1933-1942
©1999 by American Society of Hematology
585Ser in βc is phosphorylated in vivo by GM-CSF.
F.C. Stomski et al. Blood 1999;94:1933-1942
©1999 by American Society of Hematology