Development and characterization
of the common bean diversity panel
(ADP)
BREEDING LOCALLY-ADAPTED PULSE CROPS FOR
ENHANCED YIELD AND SEED QUALITIES: AN
INTEGRATED, OUTCOME-BASED PLAN FOR ARS
•
•
•
•
•
Karen Cichy
Mike Grusak
Phil Miklas
Talo Pastor-Corrales
Tim Porch
James Beaver
Consuelo Estevez
ARS
ARS
ARS
ARS
ARS
UPR
UPR
East Lansing, MI
Houston, TX
Prosser, WA
Beltsville, MD
Mayaguez, PR
Mayaguez, PR
Mayaguez, PR
Gail Wisler
Roy Scott
ARS-NPL
ARS-NPL
The main goal of the project is to increase common bean
production in Sub-Saharan Africa, through the development of
germplasm resources, molecular tools, and scientific knowledge.
OBJECTIVES
1. Develop an Andean Diversity
Panel (ADP) comprised of
300-500 dry bean lines for
genetic characterization of
targeted traits and identification of breeding materials.
2. Identify and develop Andean germplasm with improved
biological nitrogen fixation (BNF) and improved
production on degraded low input soils.
3. Improve tolerance to abiotic stresses-drought and heat.
4. Identify Andean common bean cultivars with disease
resistance genes for the control of the most widespread
and yield-reducing diseases in target countries.
OBJECTIVES
5. Determine the phenotypic
variation of seed phytic acid
,
flavonoids, and cooking time.
6. Determine the phenotypic variation and genetic control
of seed mineral concentrations, pod mineral
partitioning, and pod dry matter partitioning in Andean
beans.
Objective 1: Develop Andean Diversity Panel
Ecuador, 12
Angola, 16
Non nods, 4
Caribbean, 20
East and South
African varieties,
34
East African landraces,
120
CIAT and US core
collection, 65
N. American
(BeanCAP), 89
CIAT Africa Misc, 74
Andean diversity panel
434 entries
Increase of the ADP Panel
Subsets Increased in:
Prosser, Washington
Isabela, Puerto Rico
South Africa
4500
Objective 2: Identification of ADP germplasm efficient
under low fertility conditions
4000
3500
3000
2500
2000
1500
1000
500
0
Purgatory plot yield (kg ha-1) - Top 25 lines - Prosser, WA 2012
Morogoro, Tanzania
Trial of 95 landraces in Tanzania, 2012
Results from top 6 lines
Sub ID Name
TZ-4
pods seeds
Shoot
plant pod sd wt Yield dry wt Nodule Rust
g 100
No. No. sds kg ha
g
No.
KILOMBERO 12.6 3.7
CBB
Virus
ALS BCMV
49.5 1655
7.40
27.5
1.0
3.0
1.0
1.8
TZV-58 KIJIVU
10.3 5.0
44.0 1373
7.33
14.5
2.0
1.0
1.0
2.5
TZ-14 KIANGWE
10.8 4.2
39.8 1366
5.20
9.0
1.0
2.0
3.0
1.0
TZV-73 MASUSU
11.4 3.6
48.1 1334
8.25
17.5
2.0
1.0
2.0
1.0
TZV-41 MRONDO
14.9 4.6
37.4 1325
6.31
14.3
1.0
1.0
1.0
1.0
TZ-7
15.8 5.3
36.4 1323
5.12
20.8
1.0
2.0
1.5
1.0
BUKOBA
Correlation 0.47**
KABLANKETI
OPS-RS1
Kablanketi
KABLANKETI
SOYA
KIJIVU
SOYA
BWANASHAMBA
MRONDO
KABLANKETI
H9659-27-7
NJANO-DOLEA
Jesca
Dolly
MASUSU
OACInferno
VA-19
Kardinal
MAULASI
PinkPanther
MSOLINI
W616546
MKOKOLA
SOYA
Majesty
Yield (kg ha-1 Geomean)
Objective 3: Evaluate Abiotic Stress Response
Top 25 performers for Drought – Othello, WA 2012
Drought Intensity Index = 40%
6000
5000
4000
3000
2000
1000
0
Objective 4: Identify disease resistant germplasm
within the ADP
10.0
9.0
7.0
6.0
5.0
4.0
Blue- 3353
Red- 484A
3.0
2.0
1.0
0.0
1
10
19
28
37
46
55
64
73
82
91
100
109
118
127
136
145
154
163
172
181
190
199
208
217
226
235
244
253
262
271
280
1-9 Scale, CBB
8.0
Lines
Limited Common bacterial blight
(CBB) Resistance in ADP
Correlation coefficient between strains: 0.92
Response to strains 484A and 3353 of CBB
285 lines; 7 R., 16 Int., 262 S.
ADP
Sub-ID
Genotype
3353
484A
SAP6
SU91
635
BCV-508
OAC Redstar
4.0
2.5
+
-
430
PR-4
PR1013-3
4.5
2.5
+
-
432
PR-6
PR0637-134
4.5
2.5
+
-
435
614
PR-9
BC-315
RM-05-07
ND061106
4.3
4.0
2.8
3.0
+
+
-
32
607
96
TZ-32
BC-154
AF-1
RH No. 21
NY 105
Rojo
4.8
4.8
5.5
2.5
3.0
2.8
+
+
+
-
617
618
97
VAX 6
Morales
BC-347
BC-351
AF-2
Red Rider
AC Elk
Bilfa 4
9.0
8.5
9.0
1.7
9.0
8.0
7.0
8.8
1.3
9.0
+
-
+
-
8.6
0.8
7.7
1.1
Mean
LSD
ADP Response to NL3 Strain of BCMNV
Out of 303 lines: 37 with I gene and 3 with bc3 gene
ADP
614
635
607
605
118
427
434
121
225
599
122
113
126
632
618
629
606
623
624
630
26
51
55
97
117
123
452
456
517
600
608
609
610
616
619
622
628
Sub-ID
BC-315
BCV-508
BC-154
BC-152
AF-23
PR-1
PR-8
AF-26
CC-22
BC-76
AF-27
AF-18
AFC-7
BCV-505
BC-351
BCV-502
BC-153
BC-399
BC-401
BCV-503
TZ-26
TZV-52
TZV-56
AF-2
AF-22
AF-28
EC-6
EC-10
AG-10
BC-100
BC-175
BC-248
BC-265
BC-344
BC-367
BC-378
BCV-501
Source
WM1131-159
OT1127-57
WM1131-157
WM1131-145
OT1127-26
J.Beaver
J.Beaver
OT1127-30
05IS-0227
OT1127-44
OT1127-31
OT1127-17
OT1127-27
WM1131-148
OT1127-15
WM1131-123
WM1131-163
OT1127-55
OT1127-18
OT1122-111
OT1122-1
OT1122-16
OT1127-48
OT1127-24
OT1127-33
E.Peralta
E.Peralta
2010-TP20
OT1127-46
WM1131-118
WM1131-146
WM1129-121
WM1131-134
WM1131-158
WM1131-112
OT1127-14
Genotype
ND061106
OAC Redstar
NY 105
1132-V96
Werna
Badillo
PR0737-1
Kranskop HR-1
G 6415
Isles
Kranskop
OPS-RS4
SELIAN 05
TARS HT 1
AC Elk
H9659-27-10
NY 104
Drake
Dolly
H9659-23-1
Black Wonder
RH No. 3
KABUKU
Bilfa 4
A483
Jenny
INIAP 425
INIAP 480
Carioca, Kibala
K07921
UI-51
K-407
G-122
OAC Lyrick
UCD 0906
UCD 0701
H9659-27-7
NL3 Response
Necrosis
Necrosis
Necrosis
Necrosis
Necrosis
Necrosis
Necrosis
Necrosis
Necrosis
Necrosis
Necrosis
Necrosis
Necrosis
Necrosis
Necrosis
Necrosis
Necrosis
Necrosis
Necrosis
Necrosis
Necrosis
Necrosis
Necrosis
Necrosis
Necrosis
Necrosis
Necrosis
Necrosis
Necrosis
Necrosis
Necrosis
Necrosis
Necrosis
Necrosis
Necrosis
Necrosis
Necrosis
ENM- (bc3)
(-)
(-)
(-)
(-)
(-)
(-)
(-)
(-)
(-)
(-)
(-)
(-)
(+)
(-)
(-)
(-)
(-)
(-)
(-)
(-)
(-)
(-)
(-)
(-)
(-)
(-)
(-)
(-)
(-)
(-)
(-)
(-)
(-)
(-)
(-)
(-)
(-)
SW13 (I)
(+)
(+)
(+)
(-)
(+)
(+)
(+)
(+)
(+)
(+)
(+)
(+)
(-)
(+)
(+)
(-)
(-)
(+)
(+)
(-)
(-)
(-)
(-)
(-)
(-)
(+)
(+)
(+)
(+)
(+)
(+)
(+)
(-)
(+)
(+)
(+)
(+)
Ashy stem blight response
using Band-aid method
Combined Results
Response
Lesion length
18
Res., 1-3
0-4 cm
155
Int., 4-6
5-8 cm
88
Susc., 7-9
>= 9 cm
Notes
or dead
Objective 5: Determine the phenotypic variation of
seed phytic acid, flavonoids, and cooking time
16
Cooking Time on 100 ADP lines
14
Frequency
12
10
8
6
4
2
0
25 28 31 34 37 40 43 46 49 52 55 58 61 64 67 70 73 76
min
Objective 6: Determine the phenotypic variation and
genetic control of seed mineral concentrations
Elemental analysis of 40 Andean Entries – Seeds
Elements
Prosser Range Fold Range
Ca (mg/g DW)
K (mg/g DW)
Mg (mg/g DW)
P (mg/g DW)
S (mg/g DW)
0.7 – 2.7
11.3 – 19.9
1.4 – 2.9
4.2 – 6.8
1.9 – 2.9
Cu (µg/g DW)
Fe (µg/g DW)
Mn (µg/g DW)
Mo (µg/g DW)
Ni (µg/g DW)
Se (µg/g DW)
Zn (µg/g DW)
6.5 – 10.8
63.2 – 99.6
10.9 – 19.6
0.4 – 4.6
0.6 – 4.7
not detected
30.0 – 47.5
(Prosser)
4.1 x
1.8 x
2.1 x
1.6 x
1.5 x
1.7 x
1.6 x
1.8 x
11.0 x
7.8 x
1.6 x
Potential impact: Broad
diversity exists for seed
mineral concentrations.
Association mapping with
the ADP should lead to the
identification of seed
mineral QTLs for breeders.
SB DT1
AC Compass
USPT
ANT 1
ND060197
OAC Cygnus
Buster
Diversity Analysis
INB841
A 55G4445A
- Based on 73 informative SNPs
G51495A Beniquez
IZ102
TZV 95
ICB 12
TZV 40
Badillo
K 42
Kardinal
RoyalLisa
Red
Kamiakin
TZ 7
Blush
K 407
TZ 32
AF 1
Nuvolari
INIAP420
Fiero
Hutterite
VA 19
TZ 24
18 V96
PR9745 232
G51493A
Litekid
02Red
385Hawk
14
Isles
RedKanner
K 59
Silver Cloud
AGdeperdiz
11
Ohlio
Wallace
UCD0801
TZV
93
G23093TZV
TZ 479
TZV52
45
TZV 67
TZV
64
TZV
70
G22455
75
92
91
86
84
TZV
60
85
TZV 69
53
Katarina
K
TZV
48 94
AF 30 G23086
TZ
19TZV
G51137A
TZV TZV
77
74 89
TZ 44
2
TZV
TZ 34
33
MajestyUC Nichols
Beluga
K07921
JaloEEP558
OAC Lyrick
Isabella
Drake
FoxFire
TZV 88
Sacramento
Lassen
Red Kote
1132 V96
Montcalm
TZV
47 50
TZV 82 TZV
USDK 4
USDK CBB 15
AC Calmont
USWK CBB 17
OAC Redstar
Chinook2000
ND061106
AC Elk
G 122
TZV
TZV
5562
Erviha
UC RedKidney
BCV 507
Charlevoix
TZV 49
59
CDRK
IndJamaicaRed
TZV
73
TZV
TZV
6898
65
MICran
Fernando
TZV
56
TZV
58
PR1013 3TZV 63
TZ 26
PompadourB
UI 686
LargaComercial
TZV 97
Capri
TZV 96
Cardinal
Krimson
UI 51
TZV
21 57Dolly
Bellagio TZV
TZ
20
TZ
11
66
TZ
23
35
Cran 09
TZTZ
2216
TaylorHort
TZ 25
Camelot
1062 V98
Lark
USWK 6Red Kloud
Pink
Panther
USLK
1
NY105
CELRK NY104
UC WhiteKidney
UC Linden
TZ 14
G4564TZV 54
TZV 41
42
IZ21
TZV 71
TZV 83
G18356
BCV 500 TZV
90
UCD0701
UCD0906
81 TZV 87
AF Myasi
24 TZV
TZV
72
51
G51496A
UCD0233
PI310511
TZ 1
TZ8 35
TZ
36
TZ39
9 TZ
G13092
G20729
ICA Quimbaya
TZ 37 INIAP481
TZV
TZ
29 INIAP418
TZ
3899
TZ 10
USCR 9
TZV
43
TZ
15
TZV
61
TZ17
6 TZ 18USCR 7
TZ 31
CAL
143
ColoradodelPais
TZ 13
Etna
UCD0405
Red
Rider
Hooter
Gurabo5
TZ 27
USCR CBB 20
TZ 30
TZ 28
ADP Currently in the process of being SNP genotyped with 6,000 BeanCAP SNPs for AM Analysis
0.1
Sharing of ADP population
Development of Access Database
Concluding Comments
• Initial findings in ADP
– Limited disease resistance
– Promising efficiency under low fertility, and for BNF
– Useful range of phytonutrient concentration and cooking times
• ADP dataset will be important:
– For mapping new traits/developing markers in Andeans
– Establishing an initial baseline of African germplasm in research
and breeding efforts
– Determining research/breeding priorities focused on Africa
Acknowledgements: