University of Rochester
Medical School
Date 07/13/2010
CURRICULUM VITAE
Name
Address:
David Ian Yule
Home
Office
E-Mail
33 Corral Drive,
Penfield, NY.
Department of Pharmacology and
Physiology,
University of Rochester, Medical
School,
601 Elmwood Ave,
Rochester, NY.
Zip
14526
Phone
585-670-0394
Zip
14642
Phone
585-273-2154
FAX
585-273-2652
[email protected]
Date of Birth
11-16-1964
Place of Birth
Edinburgh, Scotland
Citizenship
British
Social Security No.
372111766
Resident Alien No
A070548059
EDUCATION
School or College
Field of Study
Degree Earned
Year
Portsmouth Polytechnic, U.K.
Pharmacology
BSc (hons)
1986
University of Liverpool, U.K.
Physiology
PhD. Mentor
D.V Gallacher
1990
POSTDOCTORAL TRAINING
M.R.C. Secretory Control Group, Physiology Department, University of Liverpool. Supervisor Prof. O.H
Petersen, (1990)
Department of Physiology, University of Michigan. Supervisor Prof. J.A.Williams. (1990-1992).
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FACULTY APPOINTMENTS
Institution
Rank
Year(s)
University of Rochester
Professor
2009-
Secondary Appointments;
Department of Internal Medicine
(Digestive Diseases and Liver
Unit) and the Center for Oral
Biology
Associate Professor
2003-2009
Unlimited Tenure
Oct 2005
Assistant Professor
1998-2003
University of Michigan
Assistant Professor
1996-1998
University of Michigan
Assistant Research Scientist
1994-1998
University of Michigan
Research Investigator
1992-1994
University of Rochester
MEMBERSHIP IN NATIONAL AND INTERNATIONAL ACADEMIC PROFESSIONAL
ORGANIZATIONS
Organization
Year(s)
American Physiological Society.
1996-present
American Gastroenterological Association
1999-present
American Society for Biochemistry and Molecular
Biology
2003-present
Biochemical Society (U.K.)
2003-present
Biophysical Society
2006-present
EDUCATIONAL CONTRIBUTIONS
Medical student teaching
1999:
Human Structure and Function, Block 4-Gastrointestinal Physiology. 2 Lectures “Digestion and Absorption I
and II.” (2 hours).
Human Structure and Function, PBL Tutor. Gastrointestinal case. (6 hours).
2000.
Human Structure and Function, Block 4-Gastrointestinal Physiology. 2 Lectures “Digestion and Absorption I
and II.” (2 hours).
Human Structure and Function, PBL Tutor. Gastrointestinal block case (6 hours.)
Renal block case. (6 Hours).
2
Endocrinology block case. (6 hours).
2001.
PBL Tutor 2nd year pharmacology case. (6 hours).
Human Structure and Function, Block 4-Gastrointestinal Physiology. 2 Lectures “Digestion and Absorption I
and II.” (2 hours).
Human Structure and Function, PBL Tutor. Gastrointestinal block case (6 hours.)
Renal block case. (6 Hours).
Endocrinology block case. (6 hours).
2002.
PBL Tutor, Year 2 case seminars pharmacology case (6 hours).
PBL Tutor, Year 2 Case seminars physiology case (6 hours).
Human Structure and Function, Block 4-Gastrointestinal Physiology. 2 Lectures “Digestion and Absorption I
and II.” (2 hours).
Human Structure and Function, PBL Tutor. Gastrointestinal block case. (6 hours).
Renal block case. (6 Hours).
2003.
PBL Tutor, Year 2 Case seminars physiology case. (6 hours).
Human Structure and Function, Block 4-Gastrointestinal Physiology. 2 Lectures “Digestion and Absorption I
and II.” (2 hours).
Human Structure and Function, PBL Tutor. Gastrointestinal block case (6 hours.)
Renal block case. (6 Hours).
2004.
PBL Tutor, Year 2 Case seminars physiology case. (6 hours).
Human Structure and Function, Block 4-Gastrointestinal Physiology. 3 Lectures; Pancreatic and Billary
Secretion” and “Digestion and Absorption I and II.” (3 hours).
Human Structure and Function, PBL Tutor. Gastrointestinal block case. (4 hours.).
Renal block case. (6 Hours).
2005.
PBL Tutor, Year 2 Case seminars physiology case. (6 hours).
Human Structure and Function, Block 4-Gastrointestinal Physiology. 3 Lectures; Pancreatic and Billary
Secretion” and “Digestion and Absorption I and II.” (3 hours).
Human Structure and Function, PBL Tutor. 4 PBLs (total 22 hrs)
2006.
Human Structure and Function, Block 4-Gastrointestinal Physiology. 4 Lectures;,“G.I Motility”, Pancreatic and
Billary Secretion” and “Digestion and Absorption I and II.” (4 hours).
Human Structure and Function, PBL Tutor. 4 PBLs (total 22 hrs)
2007
Human Structure and Function, Block 4-Gastrointestinal Physiology. 5 Lectures; “Introduction to GI
Physiology”, “G.I Motility”, Pancreatic and Billary Secretion” and “Digestion and Absorption I and II.” (5
hours).
Human Structure and Function, PBL Tutor. 2 PBLs (total 10 hrs)
3
Medical Pharmacology PBL Tutor (6 hrs)
2009
Human Structure and Function, Block 4-Gastrointestinal Physiology. 5 Lectures; “Introduction to GI
Physiology”, “G.I Motility”, Pancreatic and Billary Secretion” and “Digestion and Absorption I and II.” (5
hours).
Human Structure and Function, PBL Tutor. 2 PBL (total 10 hrs)
Graduate student teaching
1998.
IND 409. Cell Biology. Small group leader. (2 hours).
Course Director Pharmacology and Physiology Colloquium.
1999.
IND 409. Cell Biology. Small group leader. (2 hours).
IND 447. Signal Transduction. “Spatial organization of signaling” 2 lectures. (3 hours).
IND 447. Recitation “The role of targeting in signal transduction”. (1.5 hour).
2000.
IND 447. Signal Transduction. “Spatial organization of signaling” 2 lectures. (3 hours).
IND 447. Recitation “The role of targeting in signal transduction”. (1.5 hour).
2001.
IND 447. Signal Transduction. “Spatial organization of signaling” 2 lectures. (3 hours).
IND 447. Recitation “The role of targeting in signal transduction”. (1 hour).
PHP 407. “Digestion and absorption” lecture. (1 hour).
PHP550. Ion Channels and Disease. (1.5 hours)
2002.
IND 447 Signal Transduction. “Spatial organization of signaling” 2 lectures. (3 hours).
IND 447 Recitation “The role of targeting in signal transduction”. (1.5 hour).
2003.
IND 447 Signal Transduction. “Spatial organization of signaling” 2 lectures. (3 hours).
IND 447 Recitation “The role of targeting in signal transduction”. (1.5 hour).
PHP 550. Ion Channels and disease. (3 hours).
2004.
MBI 579 Salivary Glands and Saliva. “Calcium signaling in salivary acinar cells”. (1.5 hours)
IND 447 Signal Transduction. “Structure, Expression and Regulation of InsP3R”. (1.5 hours)
IND 447 Signal Transduction. “Spatial organization of signaling” 2 lectures. (3 hours).
PHP 530 Advanced Topics in Pharmacology. “The gastric system-gastric ulcers and GERD”. Facilitator (4
hours).
IND 501 Ethics and Professional Integrity in Research. (Facilitator, 2 hours).
2005.
IND 447 Signal Transduction. “Structure, Expression and Regulation of InsP3R”. (1.5 hours)
4
IND 447 Signal Transduction. “Spatial organization of signaling” 2 lectures. (3 hours).
PHP 550. Ion Channels and disease. (3 hours).
2006
IND 447 Signal Transduction. “Structure, Expression and Regulation of InsP3R”. (1.5 hours)
IND 447 Signal Transduction. “Spatial organization of signaling” 2 lectures. (3 hours).
PHP 403 Protein secretion 2 lectures (3 Hours)
2007
IND447 Signal Transduction. Course Director
IND 447 Signal Transduction. “Structure, Expression and Regulation of InsP3R”. (1.5 hours)
IND 447 Signal Transduction. “Spatial organization of signaling” 2 lectures. (3 hours).
PHP 403 Cell and Molecular Physiology. Protein secretion 2 lectures (3 Hours)
2008
IND447 Signal Transduction. Course Director
IND 447 Signal Transduction. “Structure, Expression and Regulation of InsP3R”. (1.5 hours)
IND 447 Signal Transduction. “Spatial organization of signaling” 1 lecture. (1.5 hours).
2009
IND 447 Signal Transduction. “Structure, Expression and Regulation of InsP3R”. (1.5 hours)
IND 447 Signal Transduction. “Spatial organization of signaling” 2 lectures. (3 hours).
PHP 403 Cell and Molecular Physiology. Protein secretion 2 lectures (3 Hours)
Graduate and postdoctoral training
1999
Rotation students-Hoffman Lantum, Steven Straub.
Thesis Advisor-Stephen Straub.
Postdoctoral-Dr. David Giovannucci.
2000
Thesis Advisor-Stephen Straub (Cell and Molecular Biology Training Grant).
Postdoctoral-Dr. David Giovannucci, Dr Jason Bruce.
2001
Rotation Students-Ondina Lungu, Joey Chen.
Thesis Advisor –Stephen Straub (Cell and Molecular Biology Training Grant).
Postdoctoral-Dr. David Giovannucci, Dr Jason Bruce.
2002
Rotation Students-Denia Ramirez, David Brown.
Thesis Advisor-Stephen Straub (Cell and Molecular Biology Training Grant).
Thesis Advisor-David Brown (Cell and Molecular Biology Training Grant).
Postdoctoral-Dr Jason Bruce.
2003.
Rotation students-Jenifer Butler.
Thesis Advisor-Steve Straub (Cell and Molecular Biology Training Grant).
Thesis Advisor-David Brown (Cell and Molecular Biology Training Grant).
Postdoctoral-Dr Jason Bruce.
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2004.
Rotation Students-Matt Betzenhauser, Julien Sebag, Keigan Park.
Thesis Advisor-David Brown (Cell and Molecular Biology Training Grant).
Thesis Advisor-Matt Betzenhauser (Cell and molecular biology Training Grant).
Postdoctoral-Dr Stephen Straub (Cell and Molecular Biology Training Grant).
Postdoctoral-Dr Jason Bruce.
Postdoctoral Dr Vitaliy Resnikov.
Postdoctoral Jong-Won Hak.
2005.
Rotation Students-Ryan Loy
Thesis Advisor-David Brown
Thesis Advisor-Matt Betzenhauser
Thesis CoAdvisor-Keigan Park
Postdoctoral Advisor Jong-Won Hak.
2006 .
Thesis Advisor-David Brown
Thesis Advisor-Matt Betzenhauser
Thesis CoAdvisor-Keigan Park
Postdoctoral Advisor Jong-Won Hak.
2007.
Rotation students-Meital Gabal
Thesis Advisor-Matt Betzenhauser
Thesis CoAdvisor-Keigan Park
Postdoctoral Advisor -Jong-Won Hak.
2008
Rotation student Elaina
Rotation student Wayne
Thesis Advisor-Matt Betzenhauser
Thesis Co-Advisor-Keigan Park
Postdoctoral Advisor Jong-Hak Won
Postdoctoral Advisor-Watura Masuda
SERVICE ASSIGNMENTS AND RESPONSIBILITIES OF LAST FIVE YEARS
Editorial Board
Journal of Biological Chemistry. 2003-2008.
World Journal of Gastroenterology 2004Cell Calcium 2008Editorial Advisory Board
Biochemical Journal. 2003Ad Hoc Reviewer for:
Nature
6
Science
EMBOJ
Journal of Cell Biology
Gastroenterology.
Journal of Pharmacology and Experimental Therapeutics.
Journal of General Physiology.
Journal of Clinical Investigation.
Journal of Neurochemistry.
Journal of Cellular Physiology.
Journal of Molecular Endocrinology.
Biochemica Biophysica Acta.
Proceeding of the National Academy (USA).
Cell Calcium.
Peptides.
PROFESSIONAL ADMINISTRATIVE ASSIGNMENTS AND RESPONSIBILITIES
1998. Department of Pharmacology and Physiology Admissions Committee.
1998-present. Real Time Confocal Core committee member.
1999. Cell Regulation Molecular Pharmacology Cluster Admissions committee.
2000. Cell Regulation Molecular Pharmacology Web-Page committee.
2000. Admissions Director Cell Regulation Molecular Pharmacology.
2000-present. Department of Pharmacology and Physiology Graduate Studies committee member.
2001-2006 Cellular and Molecular basis of Medicine Cluster Director.
2003- present Department of Pharmacology and Physiology faculty search committee.
2007-2009 Course Director IND 447 Signal Transduction
2007-present. Oversight Committee Confocal Microscope Core
2007-2009 Gastroenterology Division Chief Search Committee
2008-present Co-Scientific Director Confocal Microscope Core
2009-Present. Director, Cellular and Molecular Pharmacology and Physiology Program
RESEARCH ACTIVITIES OF LAST FIVE YEARS
2 R01 DK054568-10 (Yule, PI)
09/01/2008 – 04/30/2013
NIH/NIDDK
Pancreatic Function: G Protein-Mediated Ca2+ Signaling
This project involves determining if Inositol 1,4,5-trisphosphate receptor phosphorylation play an important
role in defining the differing [Ca2+]i signals stimulated by peptides and neurotransmitters in pancreatic acinar
cells.
06/01/2008 – 06/30/2012
1 RO1 DE14756 (Yule, PI)
NIH/NIDCR
[Ca2+]i and Secretory Dynamics in Parotid Acinar Cells
The long term goal of this grant is to elucidate the role of cytosolic calcium dynamics in the control of both
fluid and exocytotic secretion. The processes which underlie initiation, propagation and termination of calcium
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signals in parotid will be determined and the role these signaling characteristics play in mediating fluid and
protein secretion will be determined. No overlap.
R01 DE16999 (Shuttleworth, PI; Yule, Co-investigator)
NIH/NIDCR
Signaling pathways in salivary gland fluid secretion
01/07/2005 – 06/30/2010
The major goals of this project are to characterize and define the molecular mechanisms underlying
regulation of intracellular Ca2+ signals in the parotid acinar cells and the consequences of these events for
salivary fluid secretory mechanisms. No overlap
1 RO1 DE016960 (Begenisich, PI; Yule, Co-investigator)
NIH/NIDCR
The Physiological Roles of K Channels in Fluid Secretion
01/03/2006 – 02/28/2011
The goal of the studies is to understand the physiological roles of the Ca2+-activated K channels that are
expressed in salivary glands. This Ca2+willbe accomplished with molecular, genetic, biochemical, optical, and
electrophysiological approaches to test for their co-localization and to understand their Ca2+ sensitivities and
the mechanism of their interaction. The studies also include examining physiological fluid secretion in K
channel deficient mice and testing the applicability of the results with the mouse system to human parotid
function. No overlap.
R01 DE019245
Bengenisich/Sneyd, PI; Yule Co-Investigator
NIH/NIDCR
An Experimental/Computational Approach for Understanding salivary Fluid Secretion
08/15/08-06/30/13
This project involves the developments of a multi-scale and computational model describing the molecular
basis of fluid secretion by the salivary glands incorporating the synergies between different signaling pathways
and effector molecules, and at different levels of cellular organization – from individual acinar and duct cells, to
the prototypical secretory unit of the acinus and its associated duct. Throughout, the model predictions will be
evaluated against relevant experimental data, and will be used to develop further experiments and hypothesis of
salivary gland function.
COMPLETED.
1 PO1 DE13539 (Melvin, PI; Yule, Co-PI on Subproject 1)
04/01/2000 – 03/31/2005
NIH/NIDCR
Molecular Basis of Idiopathic Dry Mouth
Subproject 1: Salivary Gland Hypofunction: Genetic defects in Signaling Crosstalk
This project examined the hypothesis that disruptions in the cellular mechanisms responsible for the
cAMP-mediated potentiation of [Ca2+]i-dependent fluid secretion in the salivary glands underlies many cases of
idiopathic dry-mouth. To this end we are studying the biochemical, ultrastructural, and molecular basis for the
cAMP-dependent potentiation of intracellular Ca2+ signals in parotid salivary gland cells and the interactions
between these two signals on various effector proteins involved in the fluid secretory process.
R56DK054568-10 (Yule, PI)
09/01/2007-08/31/2008
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Pancreatic Function: G Protein-Mediated Ca2+ Signaling
This award represents NIH Director’s Bridging funds to support DK054568-10.
2R56DE014756-06 (Yule, PI)
07/01/2007-06/30/2008
NIH/NIDCR
[Ca2+]i and Secretory Dynamics in Parotid Acinar Cells
This award represents NIH Director’s Bridging funds to support DE14756.
INVITED LECTURES DURING LAST FIVE YEARS
Salivary Glands and Exocrine secretion Gordon Conference. Session Co-chair and Invited speaker. Ventura
CA. Feb 2005.
“Regulation of InsP3R by phosphorylation and ATP. Invited speaker. School of Biological Sciences. University
of Manchester. March 2006.
“cAMP and Calcium Signaling in Exocrine Cells” Digestive Disease Center, Yale University. March 2005.
“Calcium signaling in parotid acinar cells”- Invited speaker Stensen III symposium Ozazaki, Japan, October
2006.
Salivary Glands and Exocrine Secretion Gordon Conference. Feb 2007 Invited Speaker and Meeting Co-Chair
2007; Chair 2009.
Regulation of InsP3R by Phosphorylation and ATP. Department of Physiology, University of Oxford, UK.
March 2007.
“Regulation of InsP3R by Phosphorylation” Invited Speaker. Calcium Signaling Gordon Research Conference.
Tilton, New Hampshire. July 2007.
Ca2+ signaling dynamics in exocrine acinar cells. Invited Speaker. Microscopy and Microanalysis 2007. Ft
Lauderdale Florida. August 2007.
Regulation of InsP3R by ATP and phosphorylation. Department of Physiology. Baylor School of Medicine. Oct
2007.
Single Channel Recordings of InsP3R. Invited Speaker. Calcium and Calcium Binding Protein Meeting. La
Palma, Canary Islands, Spain. Oct 2007.
Regulation of InsP3R in Salivary Acinar Cells. NIDCR Intramural program. NIH, Bethesda MD. Feb 2008
Generating Specificity in Intracellular Ca2+ Signals. Department of Physiology, Emory University. May 2008
Regulation of Ca2+ Release in Salivary Glands. Rochester Oral Biology Conference: Salivary Glands and Saliva.
June 2008.
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Regulation of Calcium Release by ATP in pancreatic acinar cells: American Pancreatic Association. Chicago.
Nov 2008.
Cross Talk between the cAMP and Calcium Signaling Systems. Berlin Universities Insect Physiology Graduate
Program. Berlin, Germany, Nov 2008.
Chair, Gordon Research Conference; Salivary Glands and Exocrine Secretion. Galveston TX Feb 2009
Regulation of Ca2+ Release by PKA mediated Phosphorylation. Pharmacology Dept. University of North
Dakota, Grand Forks ND. March 2009.
Regulation of Ca2+ release by ATP. Current Topics in Calcium Signaling. Ouro Preto, Brazil. April 2009.
Defining Signaling Specificity by Regulation of Ca2+ Release. Calcium Signaling Gordon Conference. Barga
Italy, June 2009.
Regulation of Ca2+ Release by Cyclic Nucleotide Dependent Kinases. Calcium and Calcium Binding Protein
Meeting. Pucon, Chile, Nov 2009.
Rules for the regulation of Ca2+ release by PKG and PKA. Department of Pharmacology, University of
Cambridge, UK. April 2010.
Ca2+ Signaling Dynamics in Pancreatic Stellate Cells. FASEB Summer Conference on Calcium and Cell
Function. Steamboat Springs, CO, June 2010.
Fine Tuning Ca2+ Signaling by Regulation of Ca2+ Release. International Congress on Cell Membranes and
Oxidative Stress. Isparta, Turkey. June 2010.
MEMBERSHIP AND PARTICIPATION IN NATIONAL ADVISORY AND HEALTH COUNCILS
AND RESEARCH REVIEW COMMITTEES
Dec 1999 and Jan 2001 (resubmission). Special emphasis panel for NIH (NIDDK). (review of program project
grant entitled “Nuclear calcium signaling in Liver”).
2000-2002- Ad Hoc reviewer for the Veterans Administration.
Aug 2002-Reviewer for NIH (NIDDK) RFA “Comprehensive Programs in Beta Cell Biology”.
Aug 2002-Present Program Grant reviewer for Medical Research Council (United Kingdom).
Feb 2004-Temporary member CDF-3 NIH study section.
June 2004-Temporary member ODCS NIH study section.
Aug 2004-Member of NIH (NIDCR) spatial emphasis panel.
OCT 2004 -Member of NIH (NIDCR) spatial emphasis panel.
Nov 2004-Member of NIH (NIGMS) special emphasis panel.
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Sep 2005- Member of NIH (NIGMS) special emphasis panel (microscopy shared equipment)
Dec 2005- Member of NIH (DIDDK) special emphasis panel (PO1 submissions)
Feb 2006- Member of NIH (NIDCR) Sjogren Syndrome RFA review committee
Aug 2007- Chair of NIH (NIDCR) Special Emphasis panel on ALSG syndrome
Sep 2007-Temporary Member ODCS NIH study section.
Feb 2008-Temporary Member NIRC NIH study section.
May 2008. Member Bion-1 review group NASA study section
June 2008 Temporary Member OCDS NIH study section
June 2009-Chartered Member ODCS NIH study section.
PATENTS
"Inositol 1,4,5-Trisphosphate Receptor Mutants and Uses Thereof" Tech ID 1282 filed Jan 26th 2004.
PUBLICATIONS
Original articles
1. Yule D.I., Gallacher D.V. Oscillations of cytosolic Ca2+ in single pancreatic acinar cells stimulated by
acetylcholine. FEBS Lett. 239:358-263, 1988.
2. Martin, S.C., Yule D.I., Dunne M.J., Gallacher D.V., Petersen O.H. Vasopressin directly closes ATP
sensitive K+ channels evoking membrane depolarisation and an increase in [Ca2+]i in insulin secreting
cells. EMBO J. 8:3595-3599, 1989. (Equal primary authors).
3. Gallacher D.V., Hanley M.R., Petersen O.H., Roberts M.L., Squire-Pollard L., Yule D.I. Substance P and
bombesin elevate [Ca2+]i by different molecular mechanisms in the AR42J pancreatic acinar cell line. J.
Physiol. 426:193-207, 1990.
4. Dunne M.J., Yule D.I., Gallacher D.V., Petersen O.H. Stimulus-evoked depolarization and increase in
[Ca2+]i in insulin-secreting cells is dependent on extracellular Na+. J.Memb. Biol. 113:131-138, 1990.
5. Dunne M.J., Yule D.I. Gallacher D.V., Petersen O.H. Comparative study in the effects of chromakalim (BRL
34915) and diazoxide on membrane potential, [Ca2+]i and ATP-dependent potassium currents in insulin
secreting cells. J. Memb. Biol. 114:53-60, 1990.
6. Osipchuk U.V., Wakui M., Yule D.I., Gallacher D.V., Petersen O.H. Cytoplasmic Ca2+ oscillations evoked
by receptor stimulation, G-protein activation, internal application of inositol trisphosphate or
Ca2+: Simultaneous microfluorimetry and Ca2+ dependent Cl-current measurement in single pancratic
acinar cells. EMBO J. 9:697-704, 1990.
7. Petersen, O.H., Wakui, M., Osipchuk, Y., Yule, D.I. and Gallacher, D.V. Electrophysiology of pancreatic
acinar cells. Methods in Enzymology. 192 300-308. 1990.
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8. Yule D.I. and Williams J.A. Mastoparan induces oscillations of cytosolic Ca2+ in rat pancreatic acinar cells.
Biochem Biophys. Res. Commun. 31:159-165, 1991.
9. Dunne M.J., Yule D.I., Gallacher D.V., Petersen O.H. Effects of alanine on insulin-secreting
cells: Patchclamp and single cell intracellular Ca2+ measurements. Biochim. Biophys. Acta 1055:157164, 1991.
10. Yule D.I., Lawrie A.M., Gallacher D.V. Acetylcholine and cholecystokinin induce different patterns of
oscillating calcium signals in pancreatic acinar cells. Cell Calcium 12:45-51, 1991.
11. Petersen O.H., Gallacher D.V., Wakui M, Yule D.I., Petersen C.C., Toescu E.C. Receptor-activated
cytoplasmic Ca2+ oscillations in pancreatic acinar cells: Generation and spreading of Ca2+ signals. Cell
Calcium 12:135-144, 1991.
12. Yule D.I., Blevins G.T., Wagner A.G., Willliams J.A. Endothelin increases Ca2+ in rat pancreatic acinar
cells by intracellular release but fails to induce amylase secretion. Biocim.Biophys.Acta.1136. 175-180.
1992.
13. Wagner, A.C.C. Wishart, M., Yule, D.I. and Williams, J.A. Effects of okadaic acid on protein
phosphorylation and amylase release indicate a role for dephosphorylation in pancreatic stimulus secretion
coupling. Am.J.Physiol. 263. C1172-1180. 1992.
14. Yule, D.I. and Williams, J.A. U73122 inhibits Ca2+-oscillations in response to CCK and Carbachol but not
to JMV-180 in rat pancreatic acinar cells. J.Biol.Chem. 267.(20). 13830-13835. 1992.
15. Yule, D.I., Essington, T.E. and Williams J.A. Pilocarpine and carbachol exhibit markedly different patterns
of Ca2+ signaling in rat pancreatic acinar cells. Am.J.Physiol. (G.I.) 264. G786-G791. 1993
16. Yule, D.I., Wu, D., Essington, T.E., Shayman, J.A. and Williams, J.A. Sphingosine metabolism induces
Ca2+ oscillations in rat pancreatic acinar cells. J.Biol. Chem. 268.(17) 12353-12358. 1993.
17. R-D. Duan, Wagner, A.C.C., Yule, D.I. and Williams, J.A. Multiple inhibitory effects of genestein on
stimulus secretion coupling in rat pancreatic acini. Am.J.Physiol. 266 G303-G310. 1994.
.
18. Yule, D.I., Tseng, M-J., Williams, J.A. and Logsdon. C.D. A cloned CCKA receptor transduces multiple
signals in response to full and partial agonists. Am.J.Physiol. 265. G999-G1004. 1993.
19. Blevins, G.T., Van de Westerlo, E.M.A., Yule, D.I. and Williams, J.A. Characterization of CCKA receptor
activity by a family of CCKB receptor antagonists. J. Pharmacol. Exp. Ther. 269. 3. 911-916. 1994.
20. Yule, D.I and Williams J.A. CCKA antagonists reveal that JMV-180 and CCK bind to different sites on the
CCKA receptor. Peptides. 15. 1045-1051. 1994.
21. Yule, D.I., Kim, E. and Williams, J.A. Tyrosine kinase inhibitors attenuate capacitative Ca2+ influx in rat
pancreatic acinar cells. Biochem.Biophys.Res.Comm. 1994. 202 .1697-1704. 1994.
22. Palmer, R.K., Yule, D.I. Williams, J.A. and Fisher, S.K. Agonist -specific calcium signalling and
phosphoinositide hydrolysis in human Sk-N-MCIXC neuroepithelioma cells. J. Neurochemistry. 63.
2099-2107. 1994.
23. Simeone, D.M., Yule, D.I., Logsdon C.D. and Williams, J. A. Ca2+ signalling through secretagogue and
growth factor receptors on pancreatic AR4-2J cells. Regulatory Peptides. 55. 197-206. 1995.
24. Yang, J., Williams, J.A., Yule, D.I. and Logsdon, C.D. Mutation of carboxy terminal threonine residues in
human m3 muscarinic acetylcholine receptor modulates the extent of sequestration and desensitization.
Molecular Pharmacology. 48. 477-485. 1995.
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25. Massa, E., Kelley, K., Yule, D.I., Macdonald, R. and Uhler. M. Comparison of fura-2 imaging and
electrophysiological analysis of murine 1 subunits coexpressed with novel 2 calcium channel isoform
subunits. Molecular Pharmacology. 47:707-717. 1995.
26. Deutsch, D., Williams, J.A. and Yule, D.I. Halothane and Octanol block Ca2+ oscillations in pancreatic acini
by multiple mechanisms. Am. J. Physiol. 269 G779-G788. 1995..
27. Kimbell, E., Yule, D.I. and Mulholland, M. Extracellular ATP mediates Ca2+ signaling in cultured
myenteric neurons via a phospholipase C dependent mechanism. Am. J. Physiol. G587-G593.
270.33.1996.
28. Kimbell, E., Yule, D. I. and Mulholland, M. Caffeine and Ryanodine sensitive calcium stores in cultured
myenteric neurons. Am. J. Physiol. G594-G603. 270. 33. 1996.
29. Palmer, R.K., Yule, D.I., Williams, J.A. and Fisher, S.K. Paracrine mediation of calcium signaling in SKN-MCIXC neuroepitheliioma cells. Am.J. Physiol. 271 C43-C53. 1996.
30. Yule, D.I. Steunkel, E. and Williams, J.A. Intercellular Ca2+ waves in pancreatic acini: mechanism of
transmission. Am. J. Physiol. 271. C1285-1294. 1996.
31. Yule, D.I, Ernst, S.A., Onishi, H., and R. Wojiciewicz. Evidence that zymogen granules are not a
physiologically relevant Ca2+ pool: defining the distribution of inositol 1,4,5-trisphosphate receptors in
pancreatic acinar cells. J. Biol. Chem. 272. 9093-9098. 1997.
32. Ohnishi, H., Ernst, S.A., Yule, D.I., Baker, C. and Williams J.A. Heterotrimeric G-protein Gq/ll localized
on pancreatic zymogen granules is involved in calcium regulated amylase secretion. J. Biol. Chem. 272.
16056-16061. 1997.
33. Zhang, W. Sarosi, G, Barnhart, D. Yule, D.I. and Mulholland M. Endothelin activated calcium signaling in
enteric glia derived from neonatal guinea pig. Am. J. Physiol. 272. G1175-G1185. 1997.
34. Detjen, K. Yule, D.I., Tseng, M-J., Williams, J.A., and Logsdon, C. CCK B receptors activate similar
second messenger systems but have opposite growth effects in CHO and Swiss 3T3 cells. Am. J. Physiol.
273:C1449-57, 1997.
35. Ohnishi H. Samuelson LC. Yule D.I., Ernst SA. and Williams JA. Overexpression of Rab3D enhances
regulated amylase secretion from pancreatic acini of transgenic mice. J. Clin. Invest. 100:3044-52, 1997.
36. Giovannucci, D.R., Yule, D.I. and Stuenkel E.R. Optical measurement of stimulus-evoked membrane
dynamics in single pancreatic acinar cells. Am. J. Physiol. 275. C732-C739. 1998.
37. Yule, D.I., Baker, C.W., Park, N. and J.A. Williams. Calcium signaling in rat pancreatic acinar cells: a role
for Gq, G11 and G14. Am. J. Physiol. 276.G271-G279. 1999.
38. Wojcikiewicz, R.H., Ernst, S.A. and Yule D.I. Secretagogues cause ubiquitination and down-regulation of
inositol 1,4,5 trisphosphate receptors in rat pancreatic acinar cells. Gastroenterology. 116. 11941201.1999.
39. LeBeau*, A., Yule*, D.I. Groblewski*, G.E. and J. Sneyd. Agonist specific calcium oscillations in
pancreatic acinar cells: a role for phosphorylation of the inositol 1,4,5-trisphosphate receptor. J. General
Physiol. 113. 851-871. 1999. (*equal primary authors).
40. Sneyd, J. LeBeau, A. and Yule D.I. Traveling waves of Calcium in Pancreatic acinar cells: Model
construction and bifurcation analysis. Physica D. 145. 158-179. 2000.
13
41. Giovannucci, D.R. Groblewski, G.E. Sneyd, J. and Yule D.I. Targeted phosphorylation of inositol
trisphosphate receptors selectively inhibits localized calcium release and shapes oscillatory calcium
signals. J. Biol. Chem. 275(43) 33704-33711. 2000.
42. Straub S. Giovannucci, D.R. and Yule D.I. Calcium wave propagation in pancreatic acinar cells: functional
interaction of Inositol trisphosphate receptors, ryanodine receptors and mitochondria. J. General Physiol.
116. 547-559. 2000.
43. Beutner G, Sharma VK, Giovannucci DR, Yule D.I, Sheu SS. Identification of a ryanodine receptor in rat
heart mitochondria. J Biol Chem 276(24):21482-8. 2001.
45. Bruce, J.I.E. Shuttleworth T.J., Giovannucci DR. and Yule D.I. Phosphorylation of inositol 1,4,5trisphosphate receptors in parotid acinar cells: A mechanism for the synergistic effects of cAMP on Ca2+
signaling. J. Biol. Chem. 277. 1340-1348. 2002.
46. Giovannucci, D.R., Bruce, J.I.E., Straub, S.V., Arreola, J., Sneyd, J., Shuttleworth, T.J. and Yule D.I.
Cytosolic Ca2+ and Ca2+-activated Cl- Current Dynamics: Insights from Two Functionally Distinct Mouse
Exocrine Cells. J. Physiol. 540. 469-484. 2002.
47. Straub, S.V., D.R. Giovannucci, J.I. Bruce, and Yule, D.I. A role for phosphorylation of inositol 1,4,5trisphosphate receptors in defining calcium signals by peptide agonists in pancreatic acinar cells. J Biol.
Chem. 277: 31949-31956. 2002.
48. Bruce, J. I.E, Yule, D.I and Shuttleworth, T.J. Ca2+-dependent, protein kinase-A modulation of the plasma
membrane Ca2+-ATPase in parotid acinar cells. J. Biol. Chem. 277. 48172-488181. 2002.
49 Domotor E, Benzakour O, Griffin JH, Yule D, Fukudome K, Zlokovic BV. Activated Protein C alters
cytosolic calcium flux in human brain endothelium via binding to endothelial protein C receptor and
activation of Protease Activated Receptor. Blood. 101: 4797-4801. 2003.
50. Sneyd, J., Tsaneva-Atanaova, K., Bruce, J.I.E, Straub, S., Giovannucci, D.R. and Yule, D.I. A model for
calcium waves in pancreatic and parotid acinar cells. Biophysical J. 85. 1392-1405. 2003.
51. Brown, D.A. Melvin, J.E. and Yule, D.I. A critical role for NHE1 in intracellular pH regulation in
pancreatic acinar cells. Am. J. Physiol. 285. G804-G812. 2003.
52. Wagner L.E. Li, W-H, and Yule D.I. Phosphorylation of type-1 inositol 1,4,5-trisphosphate receptors by
cyclic nucleotide-dependent protein kinases: A mutational analysis of the functionally important sites in
the S2+ and S2- splice variants. J. Biol. Chem. 278. 45817-45817. 2003.
53. Bruce, J.I.E, Giovannucci , D.R, Blinder, G., Shuttleworth, T.J and Yule, D.I. Modulation of [Ca2+]i
signaling dynamics and metabolism by perinuclear mitochondria in mouse parotid acinar cells. J. Biol.
Chem. 279. 12909-12917. 2004.
54. Sneyd J, Tsaneva-Atanasova K, Yule DI, Thompson JL, Shuttleworth TJ. Control of calcium oscillations by
membrane fluxes. Proc. Nat. Acad. Sci (USA).101(5):1392-6. 2004.
55. Brown, D.A. Bruce, J.I.E. Straub, S.V. and Yule, D.I. c AMP potentiates ATP-evoked calcium signaling in
human parotid acinar cells. J Biol. Chem. 279: 39485 – 39494. 2004.
56. Wagner, L.E. Li, W-H., Joseph, S.K. and Yule, D.I. Functional consequences of phosphomimetic mutations
at key phosphorylation sites in the type-1 inositol 1,4,5-trisphosphate receptor. J. Biol. Chem. 2004 279:
46242-46252. 2004.
14
57 Basavappa, S., Vulapalli S.R., Zhang, H., Yule, D.I. , Coon, S. and Sundaram U. Chloride channels in the
small intestinal cell line IEC-18. J. Cell Physiol. 202(1):21-31. 2005.
58. Tsaneva-Atanasova, K. Yule, D.I. and Sneyd, J. Synchronisation of calcium oscillations in a pancreatic
acinus. Biophys J. 88(3):1535-51. 2005
59. Mignen, O., Thompson, J.L., Yule, D.I and Shutleworth, T.J. Agonist activation of ARC channels in parotid
and pancreatic acinar cells. J.Physiol. 564:791-801. 2005.
60. Chey, Y Warner, JD, Yule, D.I., and Giovannucci, D.R. Spatiotemporal analysis of exocytosis in mouse
parotid acinar cells. American Journal of Physiology (Cell Physiology). 289(5):C1209-19. 2005.
61. Sneyd, J., Tsaneva-Atanasova, K., Reznikov, V., Bai Y., Sanderson, M. and Yule , D.I. Method for
determining the dependence of calcium oscillations on inositol trisphosphate oscillations. Proc. Nat.
Acad. Sci. (USA). 103.(6) 1675-1680. 2006.
62. Khan, M.T., Wagner,L.E. Yule, D.I., Bhanumathy, C., and Joseph, S.K. Akt kinase phosphorylation of
inositol 1,4,5-trisphosphate receptors. J. Biol. Chem. Epub as doi:10.1074/jbc.M509262200.
63. Won, J-H and Yule, D.I. Measurement of Ca2+ Signaling Dynamics in Exocrine Cells with Total Internal
Reflection Microscopy. Am J Physiol Gastrointest Liver Physiol. 2006 Feb 16; Epub PMID: 16484681.
64. Wagner, L.E. Betzenhauser, M. and Yule, D.I. ATP binding to a unique site in the type-1 S2- Inositol 1,4,5trisphosphate receptor defines susceptibility to phosphorylation by protein kinase A. J. Biol.Chem. 281.
17410-17419. 2006.
65. Brown, D.A and Yule, D.I. Protein kinase C regulation of P2X3 receptors is unlikely to involve direct
receptor phosphorylation. BBA. 1773(2):166-75. 2007.
66. Gin E, Crampin, E, Brown D.A, Shuttleworth, T, Yule D.I., and Sneyd, JA. Mathematical model of fluid
secretion from a parotid acinar cell. J. Theoretical Biology. 248(1):64-80. 2007.
67. Won, J-H, Cottrell, W.J, Foster, T.E, and Yule, D.I. Ca2+ Release dynamics in parotid and pancreatic
exocrine acinar cells evoked by spatially limited flash photolysis. Am. J. Physiology. 293: G1166G1177. 2007.
68. Schug, Z., da Fonseca, P.C.A, Buanamathy, C.D, Wagner, L., Zhang, X., Bailey, B., Morris, E., Yule, D.I.
and Joseph, S. A Molecular characterization of the Inositol 1,4,5-trisphosphate receptor pore forming
segment. J. Biol.Chem. 283(5):2939-48. 2008.
69. Wagner, L.E. Joseph. SK and Yule D.I. Regulation of Single Inositol 1,4,5-Trisphosphate Receptor Channel
Activity by Protein Kinase A phosphorylation. J. Physiol. 2008 Aug 1;586 (Pt 15):3577-96. 2008.
70. Betzenhauser, M. Wagner, L.E., Mikoshiba, K and Yule, D.I. ATP modulation of Ca2+ release by type-2 and
type-3 InsP3R: differing ATP sensitivity and mechanism of regulation. J. Biol Chem. 283(31):21579-87. 2008.
72. Park, H-S, Betzenhauser, M.J., Won, J-H, Chen, J and Yule, D.I . The Type-2 InsP3 Receptor Determines
the Sensitivity of InsP3-induced Ca2+ Release to ATP in Pancreatic Acinar Cells. J Biol Chem. 283(38):2608126088. 2008.73.
73. J. D. Warner, C. G. Peters, R. Saunders, J-H Won, M.J. Betzenhauser, W.T. Gunning III, D. I. Yule & D. R.
Giovannucci. Form and Function in Organotypic Slices of the Adult Mouse Parotid Gland. Am J Physiol
Gastrointest Liver Physiol. 295(3):G629-40. 2008.
15
74. Park, KM, Yule, DI and Bowers WJ. Tumor necrosis factor-alpha potentiates intraneuronal Ca2+ signaling
via regulation of the inositol 1,4,5-trisphosphate receptor. J.Biol Chem. 283. 33069-33079. 2008.
75. M.J Betzenhauser, L. E. Wagner II, J-H Won and D. I. Yule. Studying isoform-specific inositol 1,4,5trisphosphate receptor function and regulation. Methods. 46 (3) 177-182. 2008.
76. Gin, E., Falcke, M., Wagner, L.E., Yule, D.I. Sneyd, J. Markov Chain Monte Carlo fitting of single-channel
data from inositol trisphosphate receptors. Journal of Theoretical Biology. 257. 460-474. 2009.
77. Gin, E., Falcke, M., Wagner, L.E., Yule, D.I., Sneyd. J. A kinetic model of the inositol trisphosphate
receptor based on single-channel data. Biophysical Journal. 96. (10) 4053-4062. 2009.
78. Betzenhauser, MJ; Wagner LE, Park HS and Yule D.I. ATP Regulation of Type-1 Inositol trisphosphate
Receptor activity does not require Walker A-type motifs. J. Biol. Chem. 284. 16156-16163. 2009.
79. Betzenhauser, MJ., Fike, JL., Wagner, LE., and Yule, DI. Protein kinase A increases type-2 inositol (1,4,5)trisphosphate receptor activity by phosphorylation of serine 937. J Biol Chem. 2009 Sep 11;284(37):25116-25
80. Park KM., Yule DI., Bowers, WJ. TNF-{alpha} mediated regulation of the inositol 1,4,5 triphosphate
receptor promoter. J Biol. Chem. 2009.284(40) 27557-66.
81. Park KM., Yule DI., Bowers, WJ. Impaired TNF-alpha control of IP3R-mediated Ca2+ release in Alzheimer's
disease mouse neurons. Cell Signal. 2010. 22(3) 519-26.
82. Brown DA and Yule DI., Protein kinase A regulation of P2X(4) receptors: requirement for a specific motif in the Cterminus. Biochim. Biophys. Acta. 2010. 1803(2) 275-287.
Chapters, Reviews and Symposium Contributions.
1. Williams, J.A. and Yule, D.I. Stimulus-secretion coupling in rat pancreatic acinar cells. In Pancreas: Biology,
Pathobiology and Disease. Raven Press. New York. Ed. Go, W., et al.
2. Yule, D.I. and Williams, J.A. Stimulus-secretion coupling in the pancreatic acinus. In Physiology of the
Gastrointestinal tract. Raven press. Ed. Johnson. L.R. Ch 39. pp1447-1471.
3. Palmer, R.K., Yule, D.I., McEwen, E.L. Williams, J.A., Fisher, S.K. Intra- and Intercellular calcium
signaling in human neuroepithelioma cells. J. Lipid Mediators and Cell Signaling, 14(1-3):169-74, 1996
4. Williams, J.A., Groblewski, G.E., Ohnishi, H. and Yule, D.I. Stimulus-secretion coupling of pancreatic
digestive enzyme secretion. Digestion. 58. Suppl 1:42-5, 1997.
5. Giovannucci DR, Sneyd J, Groblewski GE, Yule D.I. Modulation of InsP3 receptor properties by
phosphorylation: targeting of PKA to InsP3 receptors shapes oscillatory calcium signals in pancreatic
acinar cells. J Korean Med. Sci. Aug;15 Suppl:S55-6. 2000.
6. Yule, D.I. Subtype specific regulation of Inositol 1,4,5,-trisphosphate receptors: controlling calcium signals
in time and space. J. General Physiol. 117(5) 431-433.2001.
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7. Yule D.I., Straub S.V., Bruce J.I.E. Modulation of Ca2+ oscillations by phosphorylation of inositol 1,4,5
trisphosphate receptors. Biochem. Soc. Trans. 31 (5) 954-957. 2003.
8. Bruce J.I.E. Straub S.V., Yule D.I. Crosstalk between cAMP and Ca2+ signalling in non-excitable cells
Cell Calcium. 34. 431-444. 2003.
9. Gunter, T.E., Yule, D.I., Gunter, K.K. Eliseev, R.A. and Salter, J.D. Calcium and mitochondria. FEBS Lett.;
567(1):96-102. 2004.
10. Straub SV, Wagner, L., Bruce, J.I.E. Yule D.I. Crosstalk between calcium and cAMP signaling pathways.
Biological Research. 37(4):593-602.2004.
11. Melvin, J.E, Yule, D.I., Shuttleworth, T.J. and Begenesich, T. Regulation of fluid and electrolyte secretion
in salivary gland acinar cells. Ann. Rev Physiol.67:445-469. 2005.
12. Williams J.A. and Yule, D.I. Stimulus-Secretion Coupling in the Pancreatic Acinus. In Physiology of the
Gastrointestinal Tract. Ed L.R. Johnson. 5th Edition. Pages 1337-1369. 2006.
13. Yule DI., Betzenhauser MB., and Joseph SK. Linking structure to function: Recent lessons from inositol 1,4,5trisphosphate receptor mutagenesis. Cell Calcium. 2010. 47(6) 469-476.
14. Yule DI. Pancreatic Acinar Cells: Molecular insight from studies of signal-transduction using transgenic animals.
International Journal of Biochemistry and Cell Biology. 2010. In press.
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