From: Volatile Anesthetics Depress Calcium sup 2+ Transients and Glutamate Release in Isolated Cerebral Synaptosomes
Anesthes. 1995;83(3):593-603..
Figure Legend:
Figure 1. Calibration for glutamate release. (A) Fluorescence intensity (FI) response at 460 nm (excitation at 340 nm) versus time
with the sudden addition of glutamate (1-7 nmol) to the standard medium at 40 s, using an enzyme-coupled assay system using 50
U/ml glutamate dehydrogenase. FI increases because of the production of fluorescent product nicotinamide adenine dinucleotide
phosphate (NADPH) from NADP sup + (1 mM) in the medium. According to standard enzyme kinetics, when K mof the enzyme for
the substrate (where Km= the substrate concentration at which 50% of the maximum velocity is obtained for a constant enzyme
concentration)
exceeds
the substrateCopyright
concentration
> 20-fold,
the initial
rate of enzyme All
reaction
will be proportional to the
Date of download:
7/31/2017
© 2017byAmerican
Society
of Anesthesiologists.
rights reserved.
substrate concentration. (B) The initial slope of FI increase at 460 nm for the addition of various amounts of glutamate. Circles = the