ATCC Medium: 2917 Sea Water Base Medium
Yeast extract………………..……………….... 3.0 g
Glucose………………………………………... 20.0 g
Sea water……………………………………… 1 L
Vitamin stock………………………………….. 1.0 ml
(add aseptically after media has cooled down)
Vitamin stock
Biotin…………………………………....…. 0.005 g
Thiamine...………………………………… 0.44 g
B12…………………………………………. 0.027 g
D-Pantothenic acid hemicalcium…...…… 0.619 g
50 mM HEPES (pH 7.8)…………….……. 200 ml
Add components then filter sterilize and add aseptically to cooled autoclaved medium.