SUPPLEMENTARY INFORMATION
Synthesis, X-Ray crystal structure, DNA binding and nuclease activity of
lanthanide (III) complexes of 2-benzoylpyridine acetyl hydrazone
KARREDDULA RAJA, AKKILI SUSEELAMMA and KATREDDI HUSSAIN REDDY*
Department of Chemistry, Sri Krishnadevaraya University, Anantapur 515 003, India
e-mail: [email protected]
1.
Figure S1. X-H…π intra molecular interactions for the [La(BPAH)2(NO3)3]
2.
Figure S2. X-H…π intra molecular interactions for the [Ce(BPAH)2(NO3)(H2O)2]2NO3
(ionic nitrates are omitted for clarity)
3
Figure S3. View of the Hydrogen bond network of [Ce(BPAH)2(NO3)(H2O)2]. 2NO3
4
Figure S4. Absorption spectra of [La(BPAH)2(NO3)3] in the absence and in the presence
of increasing concentration of CT-DNA; top most spectrum is recorded in the absence of
DNA and below spectra on addition of 10 μl DNA each time
5
Figure S5. Absorption spectra of [Ce(BPAH)2(NO3)(H2O)2]. 2NO3 in the absence and in
the presence of increasing concentration of CT-DNA; top most spectrum is recorded in
the absence of DNA and below spectra on addition of 10 μl DNA each time
6
Figure S6. Agarose gel (0.8%) showing results of electrophoresis of 1 μl of pBR322
Plasmid DNA; 4 μl of Tris–HCl/NaCl (50 mM/5 mM) buffer (pH-7); 2 μl of complex in
DMF(1x10-3 M); 11 μl of sterilized water; 2 μl of H2O2 (total volume 20 μl) were added,
respectively, incubated at 37°C (30 min);
Lane 1: DNA control; Lane 2: DNA control + H2O2; Lane 3: Lanthanum complex (20
µM) + DNA; Lane 4: Lanthanum complex (40 µM) + DNA; Lane 5: Lanthanum complex
(20 µM) + DNA + H2O2; Lane 6: Lanthanum complex (40 µM) + DNA + H2O2;
7
Figure S7. Agarose gel (0.8%) showing results of electrophoresis of 1 μl of pBR322
Plasmid DNA; 4 μl of Tris–HCl/NaCl (50 mM/5 mM) buffer (pH-7); 2 μl of complex in
DMF(1x10-3 M); 11 μl of sterilized water; 2 μl of H2O2 (total volume 20 μl) were added,
respectively, incubated at 37°C (30 min);
Lane 1: DNA control; Lane 2: DNA control + H2O2; Lane 3: Cerium complex (20 µM) +
DNA; Lane 4: Cerium complex (40 µM) + DNA; Lane 5: Cerium complex (20 µM) +
DNA + H2O2; Lane 6: Cerium complex (40 µM) + DNA + H2O2;
5
Table S1. Hydrogen bonds (Å) and angles (˚) for [La(BPAH)2(NO3)3]
6
Table S2. Hydrogen bonds (Å) and angles (°) for [Ce(BPAH)2(NO3)(H2O)2]2NO3
Figure S1. X-H…π intra molecular interactions for the [La(BPAH)2(NO3)3]
Figure S2. X-H…π intra molecular interactions for the [Ce(BPAH)2(NO3)(H2O)2]2NO3(ionic
nitrates are omitted for clarity
Figure S3. View of the Hydrogen bond (dotted line) network of [Ce(BPAH)2(NO3)(H2O)2]2NO3
Figure S4. Absorption spectra of [La(BPAH)2(NO3)3] in the absence and in the presence of
increasing concentration of CT-DNA; top most spectrum is recorded in the absence of DNA and
below spectra on addition of 10 μl DNA each time
Figure S5. Absorption spectra of [Ce(BPAH)2(NO3)(H2O)2]2NO3 in the absence and in the
presence of increasing concentration of CT-DNA; top most spectrum is recorded in the absence
of DNA and below spectra on addition of 10 μl DNA each time
1
2
3
4
5
6
Figure S6. Agarose gel (0.8%) showing results of electrophoresis of 1 μl of pBR322 Plasmid
DNA; 4 μl of Tris–HCl/NaCl (50 mM/5 mM) buffer (pH-7); 2 μl of complex in DMF(1x10-3 M);
11 μl of sterilized water; 2 μl of H2O2 (total volume 20 μl) were added, respectively, incubated at
37°C (30 min);
Lane 1: DNA control; Lane 2: DNA control + H2O2; Lane 3: Lanthanum complex (20 µM) +
DNA; Lane 4: Lanthanum complex (40 µM) + DNA; Lane 5: Lanthanum complex (20 µM) +
DNA + H2O2; Lane 6: Lanthanum complex (40 µM) + DNA + H2O2;
1
2
3
4
5
6
Figure S7. Agarose gel (0.8%) showing results of electrophoresis of 1 μl of pBR322 Plasmid
DNA; 4 μl of Tris–HCl/NaCl (50 mM/5 mM) buffer (pH-7); 2 μl of complex in DMF(1x10-3 M);
11 μl of sterilized water; 2 μl of H2O2 (total volume 20 μl) were added, respectively, incubated at
37°C (30 min);
Lane 1: DNA control; Lane 2: DNA control + H2O2; Lane 3: Cerium complex (20 µM) + DNA;
Lane 4: Cerium complex (40 µM) + DNA; Lane 5: Cerium complex (20 µM) + DNA + H2O2;
Lane 6: Cerium complex (40 µM) + DNA + H2O2;
Table S1. Hydrogen bonds (Å) and angles (˚) for [La(BPAH)2(NO3)3]
__________________________________________________________________
D-H...A
d(D-H)
d(H...A)
d(D...A)
<(DHA)
N(3)-H(3A)...O(4)a
0.80(2)
2.31(2)
2.964(2)
139(2)
_________________________________________________________________
a
Symmetry transformations used to generate equivalent atoms: x, -y, z+1/2
Table S2. Hydrogen bonds (Å) and angles (°) for [Ce(BPAH)2(NO3)(H2O)2]2NO3
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D-H...A
d(D-H)
d(H...A)
d(D...A)
<(DHA)
N(3)-H(3A)...O(11)a
0.857(17)
2.00(3)
2.781(14)
151(3)
N(4)-H(4A)...O(13)b
0.842(18)
2.29(2)
3.065(4)
154(3)
N(4)-H(4A)...O(12)b
0.842(18)
2.44(4)
3.01(2)
126(3)
O(6)-H(6B)...O(10)b
0.848(18)
2.04(3)
2.836(4)
155(4)
O(6)-H(6A)...O(12)
0.840(17)
2.70(3)
3.45(2)
149(2)
O(7)-H(7A)...O(8)
0.833(18)
1.97(3)
2.734(4)
152(5)
O(7)-H(7A)...N(2)
0.833(18)
2.62(5)
3.015(4)
110(4)
O(7)-H(7A)...O(10)
0.833(18)
2.63(4)
3.237(4)
131(3)
O(7)-H(7A)...N(8)
0.833(18)
2.64(3)
3.365(4)
147(4)
O(7)-H(7B)...O(12)
0.844(19) 1.93(3)
2.77(2)
171(4)
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a
Symmetry transformations used to generate equivalent atoms: x+1, y+1/2, -z+3/2
b
Symmetry transformations used to generate equivalent atoms: -x+1, y-1/2, -z+3/2