01/15/08
01/04/08
01/11/08
01/07/08
01/12/08
01/08/08
01/09/08
01/13/08
01/10/08
01/14/08
01/04/08
01/15/08
Method 1: Counting chamber.

Cell Concentration VS Days
700
cell conectration (cells/mm3)
600
574.3333333
545.5
500
422.75
400
346
310
300
234
200
158
100
90
46
0
6-Jan
7-Jan
8-Jan
9-Jan
10-Jan
11-Jan
Days
12-Jan
13-Jan
14-Jan
15-Jan
16-Jan

Method 2: Chlorophyll content.
Chloropyll content VS Days
40
36.588
Chlorophyll content (mg/l)
35
35.567
33.568
30
28.224
25
24.102
20
17.469
15
11.770
10
5.891
5
2.347
0
6-Jan
7-Jan
8-Jan
9-Jan
10-Jan
11-Jan
Days
12-Jan
13-Jan
14-Jan
15-Jan
16-Jan
600
y = 14.017x
R² = 0.9554
500
# cells /mm3
400
300
200
100
0
-2
3
8
13
18
23
Chlorophyll content (mg/l)
Series1
Linear (Series1)
28
33
38

By Vacuum: Unsuccessful!!!!!

By evaporation:
◦ At oven at 150 C. UNSUCCESSFUL!!!

By Centrifuge:
◦ Media was centrifuge for 5 – 10 min. Too time
consuming!! But SUCCESSFUL!!!






Set up 8 or 10 bottles, (duplicates)
Monitor pH in the bottles and adjust CO2
accordingly.---Will need flowmeters for
better control of flowrate into each bottle.
Control light, using Li-Cor PAR sensor.
Monitor temperature.
Will use one bottle for growth rate
measurement, while leaving one undisturbed.
Possibly use 2 different media for the
Dunaliella algae. (Erdschreiber's Medium, F/2
medium), and for the BB.






Dry Algae
Homogenize with mortar and pestle in nhexane. Let sit for 10 min.
Centrifuge at 5000rpm for 10 min. Repeat.
Use the Dryer and Bligh method as well, (
methanol, chloroform, water mixture).
Compare.
Analyze oil.