BME Colloquium
Wide-Field Two-Photon
Fluorescence Microscopy
Speaker : DaeKeun Kim, Ph.D.
Department of Mechanical Engineering, Dankook University
The availability of lasers with femtosecond, ultrafast light pulses
provides new opportunities and challenges in instrument
design. This presentation addresses aspect of utilizing ultrafast
light pulse in optical sectioning. The fundamental characteristics
of fluorescence are introduced, and various fluorescence
microscopies are explained in the view of the implementation
by stating pros and cons. For wide-field 3D microscopy, the
basic concept of temporal focusing is introduced, and a
comprehensive mathematical model is derived for depthresolved wide-field illumination by optimizing key instrument
design parameters which enhances optical sectioning capability.
Lastly, we achieve single nanoparticle detection and super-video
rate imaging by employing high two-photon cross-section
fluorescence probe.
4:00 PM on Mar. 27th(Thu)
Engineering Building 1, E104
Prof. Woonggyu Jung(2542, [email protected])
Administration Center 2 (3606, [email protected])
School of Life Sciences