Table S8 – SIMPER analysis of the relative impact of different functional genes in
providing differences between seasons for the metatranscriptomic samples annotated
against the Hierarchy 1 SEED subsystem database. All data were randomly re-sampled
prior to analysis and the abundances were transformed by square root. Jan – January; Aug
– August; Apr – April; Av.Abund – square root of average abundance; Contrib% individual % contribution of that metabolic function to the difference between samples;
Cum.% - Cumulative % contribution of metabolic functions to difference between
samples.
Group
Apr
Group
Jan
Metabolic Function
RNA Metabolism
Unclassified
Motility and Chemotaxis
Carbohydrates
Virulence
Membrane Transport
Regulation and Cell signalling
Protein Metabolism
Clustering-based subsystems
Amino Acids and Derivatives
Cofactors, Vitamins, Prosthetic Groups,
Pigments
Stress Response
Respiration
Cell Wall and Capsule
Nucleosides and Nucleotides
Photosynthesis
Cell Division and Cell Cycle
Fatty Acids and Lipids
Secondary Metabolism
Metabolism of Aromatic Compounds
Miscellaneous
Nitrogen Metabolism
DNA Metabolism
Potassium metabolism
Macromolecular Synthesis
Phosphorus Metabolism
Sulfur Metabolism
Av.Abund
23.67
13.66
9.93
19.79
15.83
9.01
7.54
12.34
12.76
9.81
Av.Abund
17.14
8.64
2.37
25.15
14.2
3.67
5.93
11.17
16.67
13.45
Contrib%
11.42
8.6
8.41
6.94
5.98
5.93
5.16
5.11
4.32
4.06
Cum.%
11.42
20.02
28.43
35.37
41.35
47.29
52.45
57.55
61.87
65.93
9.25
7.4
5.08
10.95
5.09
1.62
5.33
1.91
0
2.32
5.97
1
6.42
1.5
0.71
6.09
3.05
Group
Apr
12.52
7.34
6.76
11.26
7.68
2.55
7.43
3.92
2.08
3.16
5.36
1
6.58
2.32
0
6.78
3.59
Group
Aug
3.88
3.34
3.08
2.87
2.82
2.78
2.46
2.39
2.26
1.89
1.13
1.1
0.94
0.9
0.79
0.75
0.68
69.81
73.16
76.24
79.11
81.92
84.7
87.17
89.55
91.81
93.71
94.84
95.94
96.89
97.79
98.58
99.32
100
Metabolic Function
Membrane Transport
Unclassified
RNA Metabolism
Motility and Chemotaxis
Cofactors, Vitamins, Prosthetic Groups,
Pigments
Protein Metabolism
Av.Abund
9.01
13.66
23.67
9.93
Av.Abund
17.38
6.75
25.63
4.42
Contrib%
13.06
8.68
7.77
6.77
Cum.%
13.06
21.74
29.52
36.29
9.25
12.34
6.31
6.86
6.57
5.66
42.86
48.52
Regulation and Cell signaling
Miscellaneous
Amino Acids and Derivatives
Carbohydrates
Stress Response
Clustering-based subsystems
Virulence
Phosphorus Metabolism
DNA Metabolism
Cell Wall and Capsule
Nucleosides and Nucleotides
Respiration
Photosynthesis
Potassium metabolism
Cell Division and Cell Cycle
Metabolism of Aromatic Compounds
Fatty Acids and Lipids
Sulfur Metabolism
Nitrogen Metabolism
Macromolecular Synthesis
Secondary Metabolism
7.54
5.97
9.81
19.79
7.4
12.76
15.83
6.09
6.42
10.95
5.09
5.08
1.62
1.5
5.33
2.32
1.91
3.05
1
0.71
0
Group
Jan
3.79
9.76
8.77
21.64
3.9
9.43
14.75
3.45
4.24
8.84
3.19
5.97
0
0
6.73
1.82
1.69
2.39
1.72
0.47
0.47
Group
Aug
4.7
4.67
4.65
4.57
4.13
3.95
3.39
2.68
2.65
2.2
1.97
1.91
1.64
1.51
1.43
1.31
1.27
0.99
0.72
0.72
0.44
53.22
57.89
62.54
67.11
71.25
75.19
78.58
81.26
83.91
86.11
88.08
89.98
91.62
93.13
94.56
95.86
97.13
98.12
98.85
99.56
100
Metabolic Function
Membrane Transport
RNA Metabolism
Cofactors, Vitamins, Prosthetic Groups,
Pigments
Clustering-based subsystems
Amino Acids and Derivatives
Carbohydrates
Virulence
Miscellaneous
Nucleosides and Nucleotides
Unclassified
Protein Metabolism
Stress Response
Phosphorus Metabolism
Cell Wall and Capsule
Motility and Chemotaxis
Respiration
DNA Metabolism
Photosynthesis
Fatty Acids and Lipids
Potassium metabolism
Metabolism of Aromatic Compounds
Regulation and Cell signaling
Cell Division and Cell Cycle
Secondary Metabolism
Sulfur Metabolism
Av.Abund
3.67
17.14
Av.Abund
17.38
25.63
Contrib%
12.77
10.91
Cum.%
12.77
23.69
12.52
16.67
13.45
25.15
14.2
5.36
7.68
8.64
11.17
7.34
6.78
11.26
2.37
6.76
6.58
2.55
3.92
2.32
3.16
5.93
7.43
2.08
3.59
6.31
9.43
8.77
21.64
14.75
9.76
3.19
6.75
6.86
3.9
3.45
8.84
4.42
5.97
4.24
0
1.69
0
1.82
3.79
6.73
0.47
2.39
6.9
6.43
5.45
4.82
4.78
4.33
3.95
3.88
3.87
3.09
2.92
2.87
2.59
2.38
2.28
2.17
2.05
2.03
1.93
1.93
1.73
1.53
1.09
30.59
37.02
42.47
47.28
52.07
56.4
60.35
64.23
68.1
71.19
74.11
76.98
79.57
81.95
84.23
86.4
88.45
90.48
92.41
94.34
96.06
97.59
98.68
Nitrogen Metabolism
Macromolecular Synthesis
1
0
1.72
0.47
0.89
0.43
99.57
100