Line
Yield
days
of
Counter
Coulter
Hemocytometer
Calibration
Average
p1
Counting
Activity
Yes
or
No
Counts
p1
Parameter
Background
Coutter
ILl
Cell
Volume
Coulter
Manometer
Suspension
Coulter
Vol
Counted
Suspension
Date/Time
Date/Time
Counted
Counted
Vol Supernatant
Tubes
Vol
Cell
Counted
Date/Time
Added Date/Time
Washed
Medium Tubes
Cells
Activity
Counting Efficiency
Model
Vial
ml
Cocktail
Cocktail
Counting
LSC
Volume/Type
of
Scintillation
Volume
Liquid
Cells
Fraction
Labeled
Date/Time
Calibration
Present
of
Date/Time
Original Calibration
Manufacturer/Lot
Radiochemical
Radiation
Half-life
Radionuclide
Modifier
Cell
Investigator
No
-10%.O01
Experiment
Date
131
p1
INo
400
500
100
200
30
1/2/02
12/21/01
12/21/01
12/20/01
0.783
date
1630
1057
958
1157
700
1630
Cobra
mm tube
Packard
12x75
None
None
0.1
12/20/01
12/13/01
Our synthesis
31ldU
0.812
8.02
1-131
None
V79
Gerashchenko
l131-10%.001
J12/20/O1
12/12
Time
Time
Time
Activity
Elapsed
Elapsed
Elapsed
Activity
Between
Beiween
Wash
hr
hr
Count hr
and
Count
Wash
MBq/ml
and
Add and
Add
Concentration
Between
MBq/ml
Original Calibration
Concentration
Since
1-1318.02
P0-210138.376
Po-2101.0
H-34500.45
H-31.0
Elapsed
Present
Time
Original
101
l-1251.47
1-12559.408
Parameters
Coulter
291.00
19.50
14.50
43.74
0.193
44.474
1-1318.02
Coulter
Coulter
1131-1
1st
10
Medium
Tube
count
160026
211412
266591
324115
159409
213713
272634
325274
379566
106010
103173
380183
24402
48125
49212
102
24993
108
91
104
2nd
CPM
12/20/01
Date
0%.001
1131-1
Experiment
0%.001
85
136
379350
318702
266269
199136
161272
102014
48720
24827
3rd
379700
322697
268498
208087
160236
103732
48686
24741
104
Average
CPM
for
379595
322593
268394
207983
160131
103628
48581
24636
control
corrected
CPM
MediumActivity
DPM
597040
507384
422138
327122
251860
162989
76410
38749
CPM/y
on
8.9646
7.6184
6.3384
4.9117
3.7817
2.4473
1.1473
0.5818
counting
jiCi/mI
At
at
9.6166
8.1725
6.7994
5.2690
4.0567
2.6253
1.2307
0.6241
addition
Cifml
Ao
at
355.8125
302.3812
251.5780
194.9519
150.0986
97.1354
45.5376
23.0928
addition
kBq/ml
Ao
Tube
Date
10
Experiment
1131-1O%.001
6690
13660
39900
34150
63370
73400
104200
6290
12820
36500
35370
64790
68750
96280
117420
32
122640
38
25
2nd
count
25
1st
CPM
Suspension
12/20/01
1131-10%.001
30
33
110900
108440
71660
60120
35480
42200
15140
7470
3rd
116987
102973
71270
62760
35000
39533
13873
6817
31
Average
CPM
for
116956
102943
71240
62730
34970
39503
13843
6786
control
corrected
CPM
183952
161912
112048
98663
55001
62131
21772
10673
CPM/y
DPM
CeilSuspension
on
0.41431
0.36467
0.25236
0.22221
0.12388
0.13994
0.04904
0.02404
0.00000
0.00000
counting
.tCVmI
A0
after
1.18124
1.03970
0.71951
0.63356
0.35319
0.39897
0.13981
0.06854
uptake
iCVmI
after
43.7057
38.4690
26.6217
23.4416
13.0679
14.7619
5.1730
2.5359
0.0000
0.0000
uptake
kBq/ml
1131-1
0%.001
CoulterSurvival
riment
1131-10%.001
e/Time
12/20/01
Tube
Couiter
10
Tube
Average
Cells/mi
Hemocytometer
3rd
3806
3721
3754
1499467
3536
3609
3624
3590
1433867
1416800
3491
3534
3616
3547
3155
2958
2959
3024
1207600
3757
3875
3852
3828
1529200
3619
3632
3576
3609
1441600
3871
3947
3987
3935
1572000
3813
3806
3829
3816
1524400
3645
3609
3718
3657
1460933
3676
3550
3594
3607
1440667
Cells
Seeded
Actual
Colony count
Cells
2nd
1st
Average
PE
3rd
Count
3rd
SF
in
Grid
4th
SF
Uncorrected
Corrected
Seeded
200
150
90
97
200
143
80
83
200
2nd
1st
3734
Predicted
10
count
2nd
1st
142
102
90
61.136
1.00
1.0000
86
46
60
67
58
40.702
0.6431
0.6658
2000
1208
123
144
149
139
11.483
0.1546
0.1878
20000
15292
168
174
194
179
1.168
0.0199
0.0191
20000
14416
37
46
47
43
0.301
0.0048
0.0049
20000
15720
30
34
37
34
0.214
0.0038
0.0035
20000
15244
20
29
30
26
0.173
0.0029
0.0028
20000
14609
15
17
22
18
0.123
0.0020
0.0020
20000
14407
27
22
22
24
0.164
0.0026
0.0027
B009171
Tube
10
Cone
263.318
302.381
303.371
174.637
355.813
149.119
194.952
90.648
251.578
150.099
17.899
0000
23.093
0.000
0.000
mBqfcell
Aclivity/Cell
12120/01
0.000
kBq/ml
Activity
Date/Time
Experiment
1131-10%.0O1
0.0026
0.0020
0.0029
0.0038
0.0048
0.6431
1.0000
Uncorrected
Survival
0.0027
0.0020
0.0028
0.0035
0.0049
0.6658
1.0000
Corrected
Survival
Li
0.01
0.1
0.001
Summary
350
50
Activity
100
Uptake
Activity
300
kBqIml
300
Concentration
Concentration
200
250
Corrected
Uncorrected
_____________
Uptake
mBq/cell
200
Cell
Uptake
150
vs
vs
Cellular
100
Survival
400
350