Supplementary Figure 1: Gating strategy and representative dot plots for flow cytometry-based
phagocytosis assay. Monocyte-derived macrophages (MDM) were generated as described. Macrophage
phagocytosis of a DLBCL cell line (Toledo) was assessed by flow cytometry (% Phagocytosis) in the
presence of 1 μM TTI-621 or Control Fc. Macrophages were defined as live, single, CD14+CD11b+ cells. %
Phagocytosis was defined as the percentage of macrophages that were VPD450+.