Supplementary Table S1 Sequence analysis of the chromosomal regions flanking O-acetyltransferase gene oac1b in S. flexneri strain 1997020
Gene coordinates
and orientation
G+C
(%)
Size
(aa)
Function or
protein
1
2
3
4
5
1..693
(666..1694)
(2092..2595)
(2606..2782)
2907..3908
52.67
53.35
55.16
52.54
39.22
230
342
167
58
333
TorR
TorT
IS
IS
Oac
6
(4199..4696)
40.36
165
Tail fiber
7
(4605..5414)
47.90
269
Tail fiber
8
(5396..6076)
50.22
226
9
(6073..7272)
53.42
399
Replication
protein P
Baseplate
10
(7273..7626)
53.11
117
Oxidoreductase
11
(7626..8366)
57.09
246
Baseplate
ORF
Database search results
Protein or origin (species, accession no.)
TorR (S. flexneri, NP_706918)
TorT (S. flexneri, NP_706917)
IS1 transposase orfB (E. coli, YP_001739974）
IS1 transposase orfA (E. coli, YP_001739973)
O-acetyl transferase (phage Sf6, NP_958191)
Acyltransferase 3 (Serratia sp. AS12, YP_004500020)
O-antigen acetylase (E. coli, ZP_08369899 )
Putative phage tail fiber protein (S. flexneri,YP_687836)
Tail fibre assembly protein (Enterobacteria phage SfV, NP_599054)
Fels-2 prophage Pin (Salmonella enterica, YP_002636669)
Bacteriophage tail protein (Salmonella enterica, ZP_03380157)
Side tail fiber protein (Salmonella enterica,YP_002040338)
Putative bacteriophage protein (Enterobacter,YP_001175554)
Replication protein P for prophage (E. coli, ZP_08355002)
Hypothetical protein SPAB_02213 (Salmonella enterica, YP_001588429)
Putative baseplate protein (Iodobacteriophage phiPLPE, YP_002128495)
Putative baseplate protein (phage LBR48, ADF83490)
Hypothetical protein SPAB_02214 (Salmonella enterica, YP_001588430)
Putative oxidoreductase (Burkholderia phage Bups phi1, ABY40544)
Putative bacteriophage protein (Salmonella enterica, YP_217574)
Predicted baseplate assembly protein (E. coli, YP_002329996)
Phage P2 baseplate assembly protein gpV (E. coli, YP_003500614)
% Identity, overlap size
and total a
100, 230, 230
100, 342, 342
99, 166, 167
100, 58, 70
91, 302, 333
46, 326, 346
36, 120, 355
94, 155, 167
90, 146, 167
84, 135, 476
86,127, 230
71, 134, 510
88, 199, 226
88, 198, 226
87, 347, 399
33, 124, 399
30, 107, 392
92, 108, 117
50, 57, 117
74, 185, 251
50, 127, 250
50, 128, 250
12
(8903..9109)
45.41
68
Unknown
13
(9260..10324)
53.62
354
Unknown
14
(10327..10632)
53.92
101
Unknown
15
(10634..11236)
52.74
200
Unknown
16
(11236..13251)
54.07
671
Lytic
transglycosylase
17
(13429..13854)
55.63
141
Tail tape
measure protein
18
(13858..14298)
53.51
146
Structural
protein
19
(14309..15454)
51.57
381
Unknown
20
21
(15458..16015)
(15990..16379)
54.66
52.56
185
129
Unknown
Head-tail
adaptor
22
(16366..16920)
57.30
184
Unknown
23
(16917..17324)
54.41
135
Structural
protein
24
25
(17290..17511)
(17554..18495)
52.7
53.72
73
313
Unknown
Capsid
Pypothetical protein SPAB_02219 (Salmonella enterica, YP_001588435)
Hypothetical protein yaldo0001_17720 (Yersinia aldovae, ZP_04621860)
Hypothetical protein SPAB_02220 (Salmonella enterica, YP_001588436)
Hypothetical protein SerAS12_2791 (Serratia, YP_004501218)
Hypothetical protein SPAB_02221 (Salmonella enterica, YP_001588437)
Hypothetical protein SerAS12_2792 (Serratia, YP_004501219)
94, 64, 114
81, 54, 117
97, 344, 354
77, 268, 353
86, 86, 101
55, 56, 101
Hypothetical protein SPAB_02222 (Salmonella enterica,YP_001588438)
Hypothetical protein ErPhphiEt88_gp19 (phage phiEt88, YP_004327322)
Lytic transglycosylase, catalytic (Salmonella enterica, YP_002040328)
Lytic transglycosylase, catalytic (Enterobacter, YP_001175545)
Hypothetical protein SPAB_02224 (Salmonella enterica, YP_001588440)
Phage tail tape measure protein (Bacillus cereus Rock4-2, ZP_04213402)
Hypothetical protein SPAB_02225 (Salmonella enterica, YP_001588441)
Structural protein (phage rv5, YP_002003554)
88, 168, 200
25, 35, 234
72, 478, 669
71, 471, 669
94, 133, 141
28, 25, 1126
99, 144, 146
36, 36, 157
Hypothetical protein E2348C_2502 (E. coli, YP_002330006)
Phage protein (phage phiEcoM-GJ1, YP_001595453)
Hypothetical protein (E. coli, CAQ99475)
Hypothetical protein ECIAI1_2638 (E. coli, YP_002388021)
99, 377, 381
40, 63, 482
99, 185, 188
100, 129, 129
Phage head-tail adaptor (Xanthobacter autotrophicus Py2,
YP_001418522)
Hypothetical protein HMPREF9533_01506 (E. coli, EGB83648)
Putative bacteriophage protein (E. coli, ZP_03001214)
Hypothetical protein ERIG_00525 (E. fergusonii, EGC08501)
Structural protein (phage SN, YP_002418833)
Hypothetical bacteriophage protein (E. coli, ZP_02999888)
Hypothetical protein E2348C_2508 (E. coli, YP_002330012)
40, 47, 123
94, 173, 184
97, 178, 184
99, 133, 135
29, 38, 155
99, 72, 73
98, 307, 313
a
26
27
(18507..19010)
(19015..20235)
53.77
54.05
167
406
Unknown
Head
28
(20250..21023)
57.24
257
Head
29
(20875..22128)
57.26
417
Portal
30
(22341..23963)
53.30
540
Terminase
31
(23966..24538)
47.47
190
Terminase
32
24910..25212
44.88
100
IS
33
34
25255..25455
25401..26078
56.22
54.57
66
225
IS
IS
35
26148..26843
48.56
231
Integrase
36
37
38
<26921..28082
28128..29267
29255..29701
379
148
Pseudogene
YccZ
Etp
50.96
52.13
Total amino acid numbers of identified proteins
Putative exported phage protein (Burkholderia cenocepacia,
YP_002233686)
Major capsid protein (Iodobacteriophage phiPLPE, YP_002128463)
Putative bacteriophage protein (E. coli , ZP_03062620)
Hypothetical protein ECIAI1_2644 (E. coli, YP_002388027)
Putative head protein (Iodobacteriophage phiPLPE,YP_002128461)
Hypothetical protein ECIAI1_2645 (E. coli, YP_002388028)
Predicted head morphogenesis protein (E. coli , YP_002330015)
Predicted portal protein (E. coli, YP_002330016)
Putative portal protein (phage Bups phi1, ABY40530)
Predicted terminase large subunit (E. coli, YP_002330017)
Putative TerL (phage Bups phi1, ABY40529)
Terminase small subunit (E. fergusonii, EGC08493)
Putative terminase small subunit (phage phiV10, YP_512255)
IS911 ORF1 (S. flexneri, NP_707660)
Gene 56 protein (phage Sf6, NP_958230)
IS911 orfB (S. flexneri, NP_836379)
IS911 orfA (S.flexneri, NP_835957)
Putative transposase OrfB (phage Sf6, NP_958229)
Integrase (phage HK022, NP_037686)
Truncated ycmA gene (S. flexneri, NP_706907)
BexD-like protein (S. flexneri, AAP16497 )
phosphotyrosine-protein phosphatase (S. flexneri, YP_688516)
58, 183, 345
22, 69, 343
98, 161, 168
96, 390, 406
36, 66, 376
95, 243, 257
99, 200, 202
99, 405, 481
54, 207, 494
99, 534, 540
65, 346, 531
90, 162, 183
52, 88, 224
100, 100, 100
100, 100, 100
100, 66, 66
100, 225, 350
100, 225, 306
99, 228, 357
100, 379, 379
100, 148, 152