Nervous Tissue Background Nervous tissue, which occurs in the brain, spinal cord, and nerves, contains neurons and neuroglial cells. The neurons are the basic structural and functional units of the nervous system involved in decision-making processes, detecting stimuli, and conducting messages. The neuroglial cells perform various supportive and protective functions for neurons. Materials Needed Textbook Use the web site: http://wps.aw.com/bc_marieb_ehap_8/25/6525/1670654.cw/index.html to view prepared microscope slides of the following: spinal cord smear dorsal root ganglion nerve (ls) Nerve (cs) Neuron model Purpose of the Exercise Review the characteristics of nervous tissue and observe neurons, neuroglial cells, and various features of the nerves. Procedure 1. Label figures 25.1 and 25.2. 2. View the online slide of a spinal cord smear. Using low-power magnification, search the slide and locate the relatively large, deeply stained cell bodies of motor neurons (multipolar neurons). 3. Observe a single motor neuron, using high-power magnification, and note (label) the following features: cell body, nucleus, nucleolus, chromatophilic substance (Nissl bodies), neurofibrils, dendrites, axon. 4. Sketch and label a single motor neuron (similar to one in notes) in the space provided in Part A. 5. View online microscope slide of a dorsal root ganglion. Search the slide and locate a cluster of sensory neuron cell bodies. You also may note bundles of nerve fibers passing among groups of neuron cell bodies. 6. Sketch and label two single sensory (afferent) neurons (a bipolar neuron) cell body in the space provided in Part A. 8. Locate online a prepared microscope slide of a nerve (ls). Locate the cross section of the nerve and note the many round nerve fibers inside. Also note the dense layer of connective tissue (perineurium) that encircles the nerve fibers and holds them together in a bundle. The individual nerve fibers are surrounded by a layer of more delicate connective tissue (endoneurium) 9. Using high-power magnification observe a single nerve fiber and note the following features: central axon, myelin around the axon, node of ranvier. 10. Sketch and label a single nerve fiber with Schwann cell (cross section) in the space provided in Part B. 11. Locate the nerve (cs) on high power. Note the following: axons, myelin, Schwann cell nucleus 12. Sketch and label a single nerve fiber with Schwann cell (longitudinal section) in the space provided in Part B. Figure 25.1 Label this diagram of a motor neuron. Figure 25.2 Label the features of the myelinated axon (nerve fiber). 4 Part A 1. Sketch and label a single motor neuron. 2. Sketch and label a single sensory neuron cell body.(bipolar) Part B 1. Sketch and label a single nerve fiber with Schwann cell (cs). 2. Sketch and label a single nerve fiber with Schwann cell (ls). Part C Match the terms in column B with the descriptions in column A. Place the letter of your choice in the space provided. Column A ___ 1. Sheath of Schwann cell containing cytoplasm and nucleus that encloses myelin Column B a. Astrocyte b. Axon ___ 2. Corresponds to rough endoplasmic reticulum in other cells c. Nissl body ___ 3. Network of fine threads within nerve fiber d. Collateral ___ 4. A membranous sheath of insulation around an axon. e. Dendrite f. Myelin ___ 5. Neuron process with many branches that conduct an impulse toward the cell body g. Neurilemma ___ 6. Branch of an axon h. Neurofibrils ___ 7. Star-shaped neuroglial cell between neurons and blood vessels (blood brain barrier) ___ 8. Nerve fiber that conducts an impulse away from the cell body Part D Match the terms in column B with the descriptions in column A. Place the letter of your choice in the space provided. Column A ___ 1. Transmits impulse from sensory to motor neuron within the central nervous system Column B a. Effector b. Ependymal ___ 2. Transmits impulse out of the brain or spinal cord to effectors ___ 3. Transmits impulse into brain or spinal cord from receptors c. Ganglion d. Interneuron e. Microglia ___ 4. Myelin-forming neuroglial cell in brain and spinal cord f. Motor neuron ___ 5. Phagocytic neuroglial cell g. Oligodendrocyte ___ 6. Structure capable of responding to motor impulse h. Sensory neuron ___ 7. Specialized mass of neuron cell bodies outside the brain or spinal cord ___ 8. Covers the inside spaces of the ventricles
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