oriT aac(3)IV
pIJ774
P1
pIJ775
P1 oriT aac(3)IV P2
P1
pIJ781
P1
pIJ782
P1
vph
vph
oriT
tet
attP
int
bla
pIJ788
bla oriT
tet
attP
int
bla
pIJ789
neo aac(3)IV neo
pIJ794
neo oriT aac(3)IV neo
pIJ795
neo oriT
pIJ796
neo oriT aadA
vph
P2
neo
neo
P2
P2
vph
P2
P2
tet
aac(3)IV oriT
FRT
P1
FRT
pIJ785
oriT
bla oriT
P2
P2 tipAp
* pIJ799
bla oriT aac(3)IV bla
** pIJ10700
P1
** pIJ10701
bla oriT
oriT
hyg
hyg
FRT
pIJ780
aadA
oriT aac(3)IV
pIJ787
FRT
P1
FRT
pIJ779
oriT aadA
FRT
P1
P2
P2
FRT
pIJ778
neo
FRT
P1
neo
FRT
pIJ777
oriT
FRT
P1
P2
SwaI
FRT
pIJ776
FRT FRT FRT FRT FRT FRT FRT
SwaI
P2
P1
egfp
FRT
oriT aac(3)IV
FRT loxP
P1
FRT loxP
pIJ773
pIJ786
FRT
REDIRECT templates
bla
* - increased carb
extension holmology
P2
** hyg promoter is
apramycin promoter