C193-E029
U l tra H ig h P erformance Liq u i d C h ro m a t o g ra p h
SPD-M30A
Photodiode Array Detector
The Shimadzu Nexera UHPLC system has evolved even further with
the Nexera X2. Incorporating the new SPD-M30A photodiode array
detector, this system provides the highest sensitivity and resolution
under a variety of conditions, whether UHPLC or HPLC.
Intelligence: The i -PDeA function separates co-eluted peaks, and enables the visualization of unseen peaks
The new analysis function, i -PDeA (intelligent Peak
Deconvolution Analysis, Patent pending), offers the user
unprecedented analysis methods. These include the resolution of
co-eluted peaks into single components, the visualization of
unseen peaks, and even the deletion of unnecessary peaks to
simplify analysis.
Even co-eluted peaks…
Are rendered as single components
in the chromatogram
Stability: Temperature-controlled TC-Optics function for obtaining even more reliable data
The SPD-M30A further strengthens baseline stability via TC-Optics (Temperature Controlled Optics), a newly designed optical system.
TC-Optics not only provides baseline stability during analysis, but also contributes to shortening the stabilization time required from
instrument startup until analysis can start. As a result, it provides more reliable data, while improving total analytical throughput.
mAU
mAU
10
SPD-M30A
254 nm
25
20
30°C
Room temperature
0
15
-10
10
25°C
5
SPD-M30A
-20
0
Other vendor’s PDA detector
-5
-30
-10
-15
-20
Other vendor’s PDA detector
0
100
200
300
400
500
600 min
-40
0
10
20
30
40
50
min
Stability with Respect to Room Temperature Shifts
Baseline After Instrument Startup
With the SPD-M30A, the baseline remains essentially
In comparison to the competing PDA detector, the baseline
constant even if the room temperature shifts.
stabilizes much faster after instrument startup.
Sensitivity and Resolution: Superior sensitivity and
resolution are achieved with the capillary cell (SR-Cell)
Broad compatibility from a single system,
from UHPLC to HPLC
The SPD-M30A also features a newly design capillary cell
The SR-Cell can be used for both UHPLC and HPLC. As a result, a
(SR-Cell), which provides both high sensitivity and high
Nexera SR system equipped with the SPD-M30A can be utilized for a
resolution. With its excellent sensitivity and wide dynamic
wide range of analyses without replacing the cell.
detection range, both the peak from the principal component
HPLC conditions
and a 0.005% infinitesimal peak can be quantified
simultaneously, as shown in the example below.
Main peak
mAU
1750
μAU
0.0
2.5
5.0
10.0
12.5
15.0
17.5
min
Faster speed
600
1250
7.5
400
S/N 9.53
UHPLC conditions
200
0
750
0.5
0.7
0.9 min
Impurity peak
250
0
0.0
0.5
1.0
min
Example of analysis when adding impurities of 0.005% for a main component
0.00
0.50
1.00
1.50
2.00
2.50
min
Example of Faster Batch Analysis of Artificial Colorants
The High-End Nexera SR System: Nexera X2 Centered on the SPD-M30A
Equipped with the SPD-M30A, the Nexera SR is a high-end system in the Nexera X2
series. The cell and the optical system have been redesigned in detail, to achieve the
highest level of sensitivity, low noise, and high resolution. In addition, it is equipped
with the new i -PDeA function that obtains separated peaks through the
deconvolution of co-eluted peaks (using the Shimadzu Workstation). With its
high-sensitivity, high-resolution capabilities, the Nexera SR could be called the
pinnacle of the Nexera X2 series.
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For Research Use Only. Not for use in diagnostic procedures.
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