Qualitative and Quantifiable Mendel 032906PHW A genetics activity with rapid cycling Brassica rapa Graphs of the patterns of inheritance in the F1 and F2 generations resulting from the cross of mothers (P1) having the qualitatively expressed gene, anthocyaninless (anl=pure green plants) with purple males (P2). Also represented in the cross is the quantifiable phenotype petite dwarf (dwf1), expressed as reduced size of plant parts. In the graphs of the cross depicted, the width of the largest cotyledon (mm) is measured on plants at 7 days after sowing. Frequency histograms of 7 day old cotyledon widths of parental (P1, P2), F1, & F2 populations graphed in various combinations to illustrate patterns of segregation of the qualitatively described trait (color) and the quantifiable, intermediately expressed trait, cotyledon size (mm). P2 P1 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 Cotyledon width,mm, 7days old P1=maternal parents, n=12, stock,C1-107, anl,Hir(1),mst2/1:1. 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 Cotyledon width,mm, 7days old P2=paternal parents n=12,stock,C1-77, dwf1,Pan(8),Hir(5) P2 F1 P1 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 Cotyledon width,mm, 7days old P1,P2,F1, F2 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 Cotyledon width,mm, 7days old F1 generation, n=12,stock,C1-139 anl/+,+/dwf1, Hir(1/5) 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 Cotyledon width,mm, 7days old F2 generation, n=36,stock,C1-140, segregating for, anl, dwf1 & Hir 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 Cotyledon width,mm, 7days old 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 Cotyledon width,mm, 7days old The parental stocks used in this activity also exhibit contrasting expression of the quantitatively inherited trait, hairs (Hir). P1 stock C1-107 as well as being anthocyaninless (anl =green) and male sterile (mst2/1:1), is hairless (Hir(1)). The P2 stock C1-77, is petite dwarf (dwf1), strongly purple, Pan(8), and moderately hairy (Hir(5)). Intensity of Pan expression is also quantitatively inherited and strongly influenced by environment. High light intenisty ,low nutrients and cool temperatures enhance Pan expression. Expression of hairs is relatively stable under various enviromnents. Detailed information on genetic decriptions of these and other stocks is at,www.fastplants.org under Rapid Cycling Brassica Collection.
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