LOCUS
2013
DEFINITION
entry
ACCESSION
VERSION
KEYWORDS
SOURCE
ORGANISM
REFERENCE
AUTHORS
Li
Exported File
5005 bp ds-DNA
circular SYN 02-MAR-
Donor vector for inserting an attB-flanked gene to generate an
vector in the Gateway(R) system.
.
.
pDONR223
synthetic DNA construct
synthetic DNA construct
1 (bases 1 to 5005)
Rual JF, Hirozane-Kishikawa T, Hao T, Bertin N, Li S, Dricot A,
N, Rosenberg J, Lamesch P, Vidalain PO, Clingingsmith TR, Hartley
JL, Esposito D, Cheo D, Moore T, Simmons B, Sequerra R, Bosak S,
Doucette-Stamm L, Le Peuch C, Vandenhaute J, Cusick ME, Albala
JS,
TITLE
JOURNAL
PUBMED
REFERENCE
AUTHORS
Hill DE, Vidal M.
Human ORFeome version 1.1: a platform for reverse proteomics.
Genome Res. 2004;14:2128-35.
15489335
2 (bases 1 to 5005)
I.M.A.G.E. Consortium
TITLE
JOURNAL
Direct Submission
Exported Saturday, Apr 06, 2013 from SnapGene 1.5.1
http://www.snapgene.com
FEATURES
Location/Qualifiers
source
1..5005
/organism="synthetic DNA construct"
/lab_host="Escherichia coli"
/mol_type="other DNA"
terminator
268..295
/note="rrnB T2 terminator"
/note="transcription terminator T2 from the E. coli rrnB
gene"
/note="color: #ffffff"
terminator
387..473
/gene="Escherichia coli rrnB"
/note="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
/note="color: #ffffff"
primer_bind
537..553
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
/note="color: #a020f0; direction: RIGHT"
protein_bind
570..801
/gene="mutant version of attP"
/bound_moiety="BP Clonase(TM)"
/note="attP1"
/note="recombination site for the Gateway(R) BP
reaction"
/note="color: #31849b"
CDS
complement(1197..1502)
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA
gyrase"
/note="ccdB"
/note="Plasmids containing the ccdB gene cannot be
propagaged in standard E. coli strains."
/note="color: #993366"
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
CDS
complement(1844..2503)
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/note="CmR"
/note="confers resistance to chloramphenicol"
/note="color: #ccffcc"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
promoter
complement(2504..2606)
/note="cat promoter"
/note="promoter of the E. coli cat gene"
/note="color: #ffffff; direction: LEFT"
protein_bind
2751..2982
/gene="mutant version of attP"
/bound_moiety="BP Clonase(TM)"
/note="attP2"
/note="recombination site for the Gateway(R) BP
reaction"
/note="color: #31849b"
promoter
complement(3001..3019)
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
/note="color: #ffffff; direction: LEFT"
primer_bind
complement(3024..3040)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
/note="color: #a020f0; direction: LEFT"
CDS
3471..4262
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase"
/note="SmR"
/note="confers resistance to spectinomycin and
streptomycin"
/note="color: #ccffcc"
/translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
rep_origin
4355..4943
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
/note="color: #ffff00"
ORIGIN
1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
2101
2161
2221
2281
2341
2401
2461
2521
2581
2641
2701
2761
2821
2881
2941
3001
3061
3121
3181
3241
3301
3361
3421
3481
3541
3601
3661
3721
3781
3841
3901
3961
4021
agctgaacga
aaaacagact
agatggtatt
gccaacttag
gcctactcgc
tgcgagcctc
catagtcctg
caagtcgttc
ctgtaatttc
attccccaga
gccacttctt
cagctttcat
agacgtgcac
tgtacatcca
atttcaccag
ctcagccatc
attctgcatg
tgatagctgt
acatacttcg
acgcatactg
actcatcgca
cggcatgatg
tgcccatggt
tggtgaaact
ggaaataggc
gccggaaatc
aaacggtgta
tacggaattc
acttgtgctt
ggttataggt
gggatatatc
ctgaaaatct
agttggaacc
tcccggtatc
gtatttattc
taataccatc
tagtctgttt
ctcgttcagc
gcaacgaaca
cctatagtga
aaaatctctg
gccaggacag
ggaaacggat
gcaagtagcg
acggcgcagt
ctcgggcatc
gcaacgatgt
cggtgatcgc
tcgaaccgac
cacacagtga
gagctttgat
gcgctgtaga
agcgcgaact
cagccacgat
ccttggtagg
aggcgctaaa
gaaatgtagt
gaaacgtaaa
acataatact
agtgacctgt
tcgaccgaca
tattgtcctc
ttttttgtgt
aaaatcatct
ggcttcatct
tactgtatcg
acatcaggtt
ccccgataac
ccccgatatg
tggccagggg
caaacagacg
tccctgttct
ccttcctgat
gttgtgctta
cgctgtcaac
ggtatacata
ttatctggct
gtactgttgt
aacctgaatc
gaaaacgggg
cacccaggga
caggttttca
gtcgtggtat
acaagggtga
cggatgagca
atttttcttt
acattgagca
aacggtggta
cgataactca
tcttacgtgc
aacagggaca
ggcgcaaagt
taagtagttg
tttatgcaaa
tttcttgtac
ggtcactatc
gtcgtattac
atgttacatt
aaatgcctcg
gaaggcacga
tatgcgctca
ggcggttttc
caagcagcaa
tacgcagcag
cgaagtatcg
gttgctggcc
tattgatttg
caacgacctt
agtcaccatt
gcaatttgga
cgacattgat
tccagcggcg
tgaaacctta
gcttacgttg
atgatataaa
gtaaaacaca
agtcgaccga
gccttccaaa
aatgccgtat
gacaaaataa
gcatcaagaa
ggattttcag
acctgcagac
aatggcgttt
ggagaccggc
caccaccggg
gatcaccatc
ataacggctc
cgtcagcaaa
tttccgcttt
ccagaccgga
tgtcactgta
tcagtatata
tttagtaagc
aattcattaa
gccagcggca
gcgaagaagt
ttggctgaga
ccgtaacacg
tcactccaga
acactatccc
ttcatcaggc
acggtcttta
actgactgaa
tatccagtga
aaaaatacgc
cgatcaacgt
ccaggattta
gcgtcgggtg
attcatagtg
atctaattta
aaagttggca
agtcaaaata
atggtcatag
gcacaagata
acttcgctgc
acccagtgga
cgcaactggt
atggcttgtt
gcgcgttacg
ggcagtcgcc
actcaactat
gtacatttgt
ctggttacgg
ttggaaactt
gttgtgcacg
gaatggcagc
ctggctatct
gaggaactct
acgctatgga
tcccgcattt
tatcaatata
acatatccag
cagccttcca
tgttcttctc
taaatcataa
aaacatctac
caatttcaca
cctctatact
tggctgtgta
ttgatgtcat
acactggcca
taaagttcac
cgtcgcccgg
tctcttttat
agagccgttc
ccagcgttcg
gatattgaca
atacgctgct
ttcttatacc
cggatccacg
gcattctgcc
tcagcacctt
tgtccatatt
cgaaaaacat
ccacatcttg
gcgatgaaaa
atatcaccag
gggcaagaat
aaaaggccgt
atgcctcaaa
tttttttctc
ccggtagtga
ctcattttcg
tttattctgc
atgctgccaa
actggatatg
atatattgat
ttataagaaa
aaatcattat
ctgtttcctg
aaaatatatc
tgcccaaggt
cataagcctg
ccagaacctt
atgactgttt
ccgtgggtcg
ctaaaacaaa
cagaggtagt
acggctccgc
tgaccgtaag
cggcttcccc
acgacatcat
gcaatgacat
tgctgacaaa
ttgatccggt
actcgccgcc
ggtacagcgc
ttaaattaga
tcactatgaa
aatgttcttc
aaacggaatc
aaagaaataa
ctattcatat
actcttatac
tactaaacgt
taagggagcc
tttcgcggtg
tatcggtggt
gggagacttt
gcgtgtcaat
aggtgtaaac
atttcaataa
gcacgcagac
tcatatatgc
tcatagcaca
gcaaaaatca
cgattacgcc
gacatggaag
gtcgccttgc
ggccacgttt
attctcaata
cgaatatatg
cgtttcagtt
ctcaccgtct
gtgaataaag
aatatccagc
atgttcttta
cattttagct
tcttatttca
ccaaaagttg
gaagtgatct
cttagtcgac
ttgtgtttta
atttatatca
gcattgctta
ttgccatcca
gcagctctgg
atcatgcctc
tgccgggtga
ttcggttcgt
gaccgaacgc
ttttggggta
atgtttgatg
gttaaacatc
tggcgtcatc
agtggatggc
gcttgatgaa
tggagagagc
tccgtggcgt
tcttgcaggt
agcaagagaa
tcctgaacag
cgactgggct
agtaaccggc
ttttgcataa
tcaactactt
gggtgatgct
gtcgtatcca
gaaaaagagg
acgctagtgt
ttttctctta
gataaagttt
tgacatttat
gctgagatca
catcatgcgc
atctgacagc
aatatcactc
cttaaactgc
accgggcgac
gacgggcttc
cttgagcaac
cctctttttg
gcgcgcaaat
ccgccctgcc
ccatcacaga
gtataatatt
aaatcaaaac
aaccctttag
tgtagaaact
tgctcatgga
ttcattgcca
gccggataaa
tgaacggtct
cgatgccatt
tccttagctc
ttatggtgaa
gcccagggct
tccgtcacag
tacaggtcac
cagtattatg
ttttacgttt
tcaatttgtt
gctgatatcc
cccgtgtctc
ctctagacca
cgcacaccgt
aagctgtaat
agcggtggta
cagtctatgc
ttatggagca
atgagggaag
gagcgccatc
ggcctgaagc
acaacgcggc
gagattctcc
tatccagcta
atcttcgagc
catagcgttg
gatctatttg
ggcgatgagc
aaaatcgcgc
4081
4141
4201
4261
4321
4381
4441
4501
4561
4621
4681
4741
4801
4861
4921
4981
//
cgaaggatgt
tacttgaagc
atcagttgga
aaccctcgag
cgtcagaccc
tctgctgctt
agctaccaac
tccttctagt
acctcgctct
ccgggttgga
gttcgtgcac
gtgagcattg
gcggcagggt
tttatagtcc
caggggggcg
tttgctggcc
cgctgccgac
tagacaggct
agaatttgtc
ccacccatga
cgtagaaaag
gcaaacaaaa
tctttttccg
gtagccgtag
gctaatcctg
ctcaagacga
acagcccagc
agaaagcgcc
cggaacagga
tgtcgggttt
gagcctatgg
ttttgctcac
tgggcaatgg
tatcttggac
cactacgtga
ccaaaatccc
atcaaaggat
aaaccaccgc
aaggtaactg
ttaggccacc
ttaccagtgg
tagttaccgg
ttggagcgaa
acgcttcccg
gagcgcacga
cgccacctct
aaaaacgcca
atgtt
agcgcctgcc
aagaagaaga
aaggcgagat
ttaacgtgag
cttcttgaga
taccagcggt
gcttcagcag
acttcaagaa
ctgctgccag
ataaggcgca
cgacctacac
aagggagaaa
gggagcttcc
gacttgagcg
gcaacgcggc
ggcccagtat
tcgcttggcc
caccaaggta
ttacgcgtcg
tccttttttt
ggtttgtttg
agcgcagata
ctctgtagca
tggcgataag
gcggtcgggc
cgaactgaga
ggcggacagg
agggggaaac
tcgatttttg
ctttttacgg
cagcccgtca
tcgcgcgcag
gtcggcaaat
ttccactgag
ctgcgcgtaa
ccggatcaag
ccaaatactg
ccgcctacat
tcgtgtctta
tgaacggggg
tacctacagc
tatccggtaa
gcctggtatc
tgatgctcgt
ttcctggcct