FIGURE S1
Generating starting (unselected) populations for experimental evolution
genotype
Females
white
Dahomey
WhiteDahomey
WhiteDahomey
heterozygote
female offspring
(orange eyes)
Df(1)Exel6234
White
heterozygote
female offspring
(orange eyes)
Males
Df(1)Exel6234
White
Female
Male
Df(1)Exel6234
Y
x
Df(1)Exel6234
Y
x
WhiteDahomey
Y
S1
S2
S3
S4
SPRDeficiency (red eyes)
cross to white Dahomey
stock males
(repeated x 4 generations)
Df(1)Exel6234
Y
x
SPR- population
Df(1)Exel6234
Df(1)Exel6234
genotype
cross to either SPR- or
white male offspring
White
Y
Control population
C1
C2
C3
C4
White
White
Female
White
Y
Male
unselected SPR- (redeyed) and control
(white-eyed)
populations
replicate experimental
evolution lines
FIGURE S2
Creating extra controls for sperm competition experiments: “SPR+ evolved controls”
C1
female
C1, C2, C3
or C4
White
White
C2
C3
C4
Female
White
Y
Male
Df(1)Exel6234
Y
x
heterozygote
Df(1)Exel6234
White
female offspring
C1, C2, C3 or C4
White
White
White
Y
x
heterozygote
female offspring Df(1)Exel6234
White
C1, C2, C3 or C4
x
Df(1)Exel6234
Df(1)Exel6234
C1
C2
C3
C4
SPR+ evolved (control,
white-eyed) populations
cross to SPR- (unselected,
red-eyed) male
cross to males from same
evolved control line
(C1, C2, C3 or C4)
repeated x 6 generations
Df(1)Exel6234
Y
cross to SPR- male
offspring from same
line and cross
Df(1)Exel6234
Y
SPR- (red-eyed) flies in
SPR+ (control) evolved
genetic background
Df(1)Exel6234
Df(1)Exel6234
Female
Df(1)Exel6234
Y
Male
”SPR+ evolved control”
populations
FIGURE S3
Creating extra controls for sperm competition experiments: “SPR- evolved controls”
S1
female
S1, S2, S3
or S4
Df(1)Exel6234
White
heterozygote
Df(1)Exel6234
White
female offspring
S1, S2, S3 or S4
S2
S3
S4
Df(1)Exel6234
Df(1)Exel6234
Female
Df(1)Exel6234
Y
Male
cross to white Dahomey
stock male
x
WhiteDahomey
Y
x
Df(1)Exel6234
Y
x
White
Y
cross to white male
offspring from same
line and cross
White
Y
white-eyed flies in
SPR- evolved genetic
background
heterozygote
female offspring Df(1)Exel6234
White
S1, S2, S3 or S4
White
White
S1
SPR- evolved
populations (red eyes)
S2
S3
S4
White
White
Female
White
Y
Male
cross to males from same
evolved line
(S1, S2, S3 or S4)
repeated x 6 generations
”SPR- evolved control”
populations (white
eyed)