Supplementary Figures
Figure S1: EGFP fluorescence decay and instrument response function (IRF) acquired and
analyzed using Becker & Hickl SPC-830 TCSPC- FLIM system with SPC Image software.
0.36
2.90
2.95
0.34
50% Glycerol
3.00
0.33
3.05
25% Glycerol
3.10
0.32
3.15
water
3.20
1.75 1.80 1.85 1.90 1.95 2.00 2.05 2.10
n2
τ/ ns
1/τ/ ns-1
0.35
2.80
Experimental data
Linear fit of experimental data
2.85
75% Glycerol
Figure S2: (a) Fluorescence lifetime of Rhodamin 6 in Glycerol solutions. Chart demonstrates a
strict correlation between fluorescence lifetime and refractive index of fluorophore environment
Error bars are S.D.
Figure S2 (b) Fluorescence lifetime of YOYO-DNA in solutions with different refractive index (n).
Error bars are S.D.