Supplementary Data
Supplementary Table 1
Clinico-pathological parameters of the selected cohort of patients
Please, note that due to the missing information not all numbers sum up to 411.
BM indicates bone marrow, DTC – disseminated tumor cell, neg – negative, pos –
positive, TNBC – triple negative breast cancer
Clinico-pathologic
Status
Total
cohort
parameters
n
Age
median=58
(years)
range
menopause
T status
N status
M status
%
28-85
<median
206
50.2
>=median
204
49.8
total
410
premenopause
105
26.3
perimeopause
18
4.5
postmenopause
277
69.3
total
400
T1
217
53.2
T2
156
38.2
T3
21
5.1
T4
14
3.4
total
408
N0
255
62.5
N1-3
153
37.5
total
408
M0
386
96.5
M1
14
3.5
total
400
Metastatic
no
335
83.5
relapse
yes
66
16.5
total
401
DTCs (-)
297
74.3
DTCs (+)
103
25.8
BM status
total
400
TNM
IA
162
40.7
staging
IIA
117
29.4
IIB
65
16.3
IIIA
21
5.3
IIIB
12
3
IIIC
7
1.8
14
3.5
IV
total
Grade
well differentiated (G1)
398
32
8
moderately differentiated (G2)
221
55.3
poorly differentiated (G3)
147
36.8
total
400
Clinico-pathologic
Status
Total
cohort
parameters
Vascular invasion
Recurrence
Death
HR
n
%
342
91.2
yes
33
8.8
total
375
no
296
76.7
yes
90
23.3
total
386
no
341
84.6
yes
62
15.4
total
403
no
negative
71
17.3
positive
340
82.7
total
411
Her2
negative
349
94.1
overexpression
positive
22
5.9
total
371
Molecular
luminal A
136
38.1
subtype
luminal B
159
44.5
61
17.1
1
0.3
TNBC
Her2-postive
Ki-67
ALDH1
total
357
neg
166
45.4
pos
200
54.6
total
366
neg
268
90.5
pos
28
9.5
total
296
Supplementary Table 2 Clinico-pathological parameters of selected cohort of
patients for CTC analysis
Please, note that due to the missing information not all numbers sum up to 100.
Clinico-pathologic
Status
n
parameters
Age
median=66
(years)
range
T status
N status
M status
Grade
Total cohort
%
41-83
<median
51
52.0
>=median
47
48.0
total
98
T1
31
39.2
T2
29
36.7
T3
6
7.6
T4
13
16.5
total
79
N0
26
33.8
N1-3
51
66.2
total
77
M0
33
60.0
M1
22
40.0
total
55
5
7.5
moderately differentiated (G2)
32
47.8
poorly differentiated (G3)
30
44.8
total
67
negative
16
16.3
positive
82
83.7
total
98
negative
60
68.2
positive
28
31.8
total
88
Metastatic
no
69
69.0
Progression
yes
31
31.0
total
100
HR
Her2
Death
Metastasis locus
Therapy
well differentiated (G1)
No
85
85.0
Yes
15
15.0
total
100
brain
3
3.1
bone
65
67.7
liver
7
7.3
lung
13
13.5
no
7
7.3
others
1
1.0
total
96
bisphosponates
37
44.0
denosumab
9
10.7
osteoclast inhibitor
1
1.2
other chemotherapy
37
44.0
total
84
Supplementary Table 3 Different cut-offs tested to select the optimal one for
statistical analysis
Cut-off for Jagged 1 in tumor cells
based on
neg vs. pos
intensity
no vs. weak vs. moderate vs. strong
intensity
no, weak vs. moderate, strong
intensity
no, weak, moderate vs. strong
intensity
no vs. weak, moderate, strong
index score (maximal result for two tumor samples)
< mean index score vs. > mean index score
index score (maximal result for two tumor samples)
< median index score vs. > median index score
index score (sum for two tumor samples)
< mean index score vs. > mean index score
Cut-off for Jagged 1 in stromal cells
based on
neg vs. pos
intensity
no vs. weak vs. moderate vs. strong
intensity
no, weak vs. moderate, strong
intensity
no, weak, moderate vs. strong
intensity
no vs. weak, moderate, strong
index score (maximal result for two tumor samples)
< mean index score vs. > mean index score
index score (maximal result for two tumor samples)
< median index score vs. > median index score
index score (sum for two tumor samples)
< mean index score vs. > mean index score
Blue color indicates cut-offs selected for the current study as optimal.
Supplementary Table 4 Correlation of stromal Jagged 1 to proliferation marker Ki67 expression in tumor cells
F indicates Fisher exact test, neg – negative, pos - positive
Clinico-pathologic
neg
parameters
Ki-67
Jagged1 in stroma
Status
pos
p-value
n
%
n
%
neg
90
45.5
0
0.0
pos
108
54.5
6
100.0
0.035 (F)
Supplementary Figure 1 Intensity of Jagged 1 expression
Supplementary Figure 2 Correlation of Jagged 1 staining intensity and the
percentage of positive tumor cells