Norovirus Prevention
PURE Hard Surface-The Cruise Line Solution
Presented to
Exclusively manufactured and
marketed by Intercon Chemical /
Clearly Better Solutions
Protect your brands!
Forward-Looking Statements
Any statements contained in this presentation that do not describe historical facts may constitute
forward-looking statements as that term is defined in the Private Securities Litigation Reform
Act of 1995. Forward-looking statements inherently involve risks and uncertainties that could
cause our actual results to differ materially from the forward-looking statements. Factors that
could cause or contribute to such differences include, but are not limited to: the Company’s cash
position and liquidity requirement;, the Company’s failure to implement or otherwise achieve
the benefits of its proposed business initiatives and plans; acceptance of the Company's current
and future products and services in the marketplace, including acceptance of the Company’s
Hard Surface disinfectant by SUBWAY® franchisees; the ability of the Company to develop
effective new products and receive regulatory approvals of such products, including the
required regulatory approvals to use its SDC-based technology as a direct food contact
processing aid; competitive factors; dependence upon third-party vendors, and other risks
detailed in the Company's periodic report filings with the Securities and Exchange Commission;
including our report on Form 10-Q filed on December 11, 2014. You should not place undue
reliance on these forward-looking statements, which speak only as of the date of this press
release. By making these forward-looking statements, the Company undertakes no obligation
to update these statements for revisions or changes after the date of this presentation.
Preventing the smallest things from damaging
your biggest asset…your brand!
Agenda
Norovirus
Prevention
PURE
Hard Surface
Current On
Board Use
Other Public
Health Organisms
3
Norovirus: A Real Public Health Issue

Scope of the Norovirus Issue




How Norovirus Outbreaks Occur





“Noroviruses are the leading cause of reported outbreaks of gastroenteritis in the US”
“About 20 million people get sick from Norovirus each year”
“64% of the Norovirus outbreaks from food contamination are attributed to restaurant
settings”
Infected passengers board ship and introduce organisms to high touch surfaces
(handrails, elevator buttons, door handles, dinning chair and table surfaces, restroom
surfaces, deck and theatre chairs, etc.)
“Infected employees touch food or food contact surfaces: most infectious during illness
and remain infectious for 2 weeks after symptoms cease”
“Food is placed on infected surfaces”
“Virus is aerosolized from an infected employee to food or food contact surface”
Preventative Measures in Restaurant Environments to Control Norovirus




“All HIGH-TOUCH SURFACES IN PUBLIC AREAS should be CONSISTENTLY DISINFECTED
THROUGHOUT THE CRIUSE”
“Employees should not prepare or be in food preparation areas during illness”
“All employees should wash hands thoroughly and frequently”
“Clean and DISINFECT utensils, counters, equipment and surfaces routinely”
4
PURE Hard Surface: The Cruise Line Solution
Rapid
Efficacy
User
Friendly
Does not
Promote
Resistance
Residual
Protection
Nonhazardous/
Odorless
5
SDC Dual Modes of Action
Silver dihydrogen citrate’s rapid and broad spectrum efficacy is largely attributed to its dual
mechanisms of action and unique characteristics. These two mechanisms, alone or in
combination, make SDC a powerful antimicrobial while mitigating microbial resistance.
Bactericidal/Fungicidal Action SDC utilizes a multiple prong attack against the cell
wall and DNA of microorganisms. SDC targets an organism’s cell wall (cell membrane)
because silver ions are highly attracted to sulfur-containing thiol groups found in
metabolic and structural proteins bound to the membrane surface. SDC targets these
critical proteins and destroys their structure. This disruption of the organism ’ s
membrane function and integrity lyses the membrane and the organism dies.
Citrate, in addition to stabilizing silver ions, plays a key part in the antimicrobial
effectiveness of SDC. Bacteria are actually attracted to SDC because citric acid is a
food source for them. This allows SDC to easily enter the microorganism through
membrane transport proteins. Once inside the organism, SDC binds to DNA and
intracellular proteins causing irreversible damage to the DNA and protein
structure. Metabolic and reproductive functions halt, and the organism dies as a result
of this “Trojan Horse” attack.
Virucidal Action Viruses are much smaller than bacterial and present fewer targets on
which a biocide can act. SDC efficacy against enveloped and non-enveloped viruses
stems from its ability to target both the viral envelope, or capsid, and the viral nucleic
acid. Silver destroys not only the viral envelope, preventing the virus from attaching to a
host cell, it also destroys the infectious component of the virus, the nucleic acid.
6
Rapid Elimination of Viruses
VIRAL TEST METHODS: The US EPA accepts carrier based virucidal test methods to support virucidal activity of a disinfectant which
are modifications of the AOAC Use Dilution Test or the AOAC Germicidal Spray Products Test. Each virus claimed must be tested in an
appropriate test system using a cell line which supports the growth of the virus. The method as outlined in the EPA Disinfectant Technical
Science section (DIS-TSS 07) states:
To simulate in-use conditions, the specific virus to be treated must be inoculated onto hard surfaces, allowed to dry, and then treated with
the product according to the directions for use on the product label. One surface for each of two different batches of disinfectant must be
tested against a recoverable virus titer of at least 104 from the test surface (petri dish, glass slide, steel cylinder, etc.) for a specified
exposure period at room temperature. The virus is then assayed by an appropriate virological technique.
Organism
Murine Norovirus (MNV-1.CW1)
Norovirus -as Feline Calicivirus (ATCC VR-782)
HIV type 1- Strain HTLV IIIB
Herpes Simplex Type 1 VR-733 F(1) Strain (ATCC VR-733)
Rotavirus (Strain WA, Ottawa)
Human Coronavirus (ATCC VR-740)
Influenza A (H1N1) (ATCC VR-1469)
Swine Influenza A (H1N1) (ATCC VR-333)
Respiratory Syncytial Virus (ATCC VR-26)
Adenovirus Type 2 (ATCC VR-846)
Avian Influenza A (ATCC VR-2072)
Influenza A (ATCC VR-544)
Rhinovirus (ATCC VR-1147)
Polio Type 2 (ATCC VR-1002)
Hepatitis B Virus (HBV)
Hepatitis C Virus (HCV)
Dried
Virus
Control
6.5
10
6.0
10
5.25
10
6.0
10
4.5
10
4.5
10
6.5
10
6.75
10
4.75
10
6.0
10
4.75
10
6.5
10
4.5
10
4.5
10
6.0
10
5.25
10
Log
Reduction
≥6.0
≥5.88
≥3.75
≥5.5
≥4.0
≥4.0
≥6.0
≥6.25
≥4.25
≥5.5
≥4.25
≥6.0
≥4.0
≥3.0
≥5.79
≥4.93
Contact
Time
60 seconds
60 seconds
30 seconds
60 seconds
30 seconds
30 seconds
30 seconds
30 seconds
30 seconds
30 seconds
30 seconds
30 seconds
60 seconds
60 seconds
60 seconds
7
60 seconds
Rapid Elimination of Bacteria
BACTERIAL TEST METHODS: For registration with the US Environmental Protection Agency (US EPA), disinfection efficacy is
tested following either the AOAC Use Dilution Test Method or the AOAC Germicidal Spray Products Test Method. In each method,
cultures of bacteria are dried onto a number of small carriers (stainless steel penicylinders in the Use-Dilution test or glass slides in
the AOAC Germicidal Spray Products Test). Once dried, the carriers must contain a bacteria concentration of at least 104. These
carriers are exposed to the disinfectant for a specified contact time and then transferred to test tubes containing growth medium and
a neutralizing agent to stop the action of the disinfectant. The carriers are incubated for 48 hours. The tubes are then examined for
growth or no growth. To pass a 60 carrier test, three batches are tested and 59 out of 60 carriers must show no growth for each
product batch. To pass a 10 carrier test, two batches are tested and all 10 carriers must show no growth.
Organism
Pseudomonas aeruginosa (ATCC#15442)
Staphylococcus aureus (ATCC#6538)
Salmonella enterica (ATCC#10708)
Listeria monocytogenes (ATCC#19111)
Vancomycin resistant Enterococcus faecium (VRE) (ATCC#700221)
Methicillin resistant Staphylococcus aureus (MRSA) (ATCC#700698)
Community Associated MRSA (CA-MRSA) (NRS123, USA 400)
Community Associated MRSA (CA-MRSA-PVL) (NRS 192)
Escherichia coli O157:H7 (ATCC#43888)
Acinetobacter baumannii (ATCC#19606)
Campylobacter jejuni (ATCC#29428)
Carbapenem resistant Escherichia coli
Carbapenem resistant Klebsiella pneumoniae
Carbapenem resistant Klebsiella pneumonia, NDM-1
# of Carriers
Exposed Showing
Growth
180
0
180
2
180
0
20
0
20
0
20
0
20
0
20
0
20
0
20
0
20
0
20
0
20
0
20
0
Contact
Time
30 seconds
2 minutes
30 seconds
2 minutes
2 minutes
2 minutes
2 minutes
2 minutes
2 minutes
2 minutes
2 minutes
2 minutes
2 minutes
2 minutes
Carrier
Population
6
10
6
10
5
10
6
10
4.5
10
5
10
6
10
6
10
4.5
10
6
10
6
10
5
10
5
10
5
10
8
High Performing Food Contact Surface Sanitizer
FOOD CONTACT SURFACE SANITIZER TEST METHODS: Sanitizers applied to food contact surfaces are defined as incidental food
additives under the Federal Food, Drug, and Cosmetic Act, as amended (21 U.S.C. 201 et seq.), and require establishment of a food
additive tolerance or an exemption for the need for a tolerance. In addition, to support registration with the US EPA, the product must
show efficacy following the AOAC Germicidal and Detergent Sanitizing Action of Disinfectants (AOAC 960.09) test method.
In this method, cultures of each test organism are grown in an appropriate broth and grown to an acceptable concentration. In duplicate
for each test organism, 1 mL of the concentrated culture is inoculated into a flask containing 99 mL of the test product for the contact time
of 30 seconds. After the contact time has elapsed, aliquots of the organism/test substance are neutralized and plated to analyze for
surviving microorganisms as compared to the untreated control. Acceptable results must demonstrate a 99.999%reduction in the number
of microorganisms within 30 seconds. The EPA requires that labels directions state a minimum contact time of 1 minute for sanitization of
food contact surfaces.
Organism
Staphylococcus aureus
Escherichia coli
Dried Virus
Control
7
8 x 10
7
9.4 x 10
Log
Reduction
7
≥10
7
≥10
Contact Time
30 seconds
30 seconds
%
Reduction
>99.999
>99.999
• NSF Registration assures inspection officials and
end users that formulations and labels meet
appropriate food safety regulations.
• NSF Registration is based on the NSF Registration
Guidelines (formerly USDA Guidelines for Obtaining
Authorization of Compounds to be Used in Meat and
Poultry Plants).
9
Residual Efficacy
Residual Efficacy has been demonstrated in multiple lab tests
Evaluation of Residual Efficacy on Treated Surfaces
 Product applied to glass slides with a clean towel
 Glass slides
 Staphylocoous aureus, Salmonella enterica and Pseudomonas aeruginosa
 3-log reduction up to 24-hours after surfaces were treated
Confirmatory surface testing - Misting Study on Inoculated Surfaces
 Product misted onto surfaces
 Glass, stainless steel pitted, stainless steel 316, linoleum, plastic resin
(Horizontal and vertical surfaces)
 E. coli, S. aureus, Salmonella, Listeria
 5 log reduction in 30 seconds
 24-hour residual showed 3-log reduction against all organisms on all
surfaces
10
Study Reports
• Full Test Reports available
online at
• http://technical.purebio.com
• Performed at independent,
third party labs
• ATS Labs
• Nelson Labs
• Bioscience Labs
• Deibel Labs
11
EPA Toxicity Categories
Study
Category IV
(None)
Category III
(Caution)
Category II
(Warning)
Category I
(Danger)
SIGNAL WORD
NONE REQUIRED
CAUTION
WARNING
DANGER
FIRST AID STATEMENTS &
HAZARD WARNING
NONE REQUIRED
REQUIRED
REQUIRED
REQUIRED
Acute Oral
> 5000 mg/kg
> 500 thru 5000 mg/kg
> 50 thru 500 mg/kg
Up to and including 50 mg/kg
Acute Dermal
> 5000 mg/kg
> 2000 thru 5000 mg/kg
> 200 thru 2000 mg/kg
Up to and including 200 mg/kg
Acute Inhalation1
> 2 mg/liter
> 0.5 thru 2 mg/liter
> 0.05 thru 0.5 mg/liter
Up to and including 0.05 mg/liter
Primary Eye Irritation
Minimal effects clearing in less Corneal involvement or other eye
than 24 hours
irritation clearing in 7 days or less
Primary Skin Irritation
Mild or slight irritation at 72
hours (no irritation or slight
erythema)
Moderate irritation at 72 hours
(moderate erythema)
PURE Hard Surface/
Oxivir TB
Virox 5
Lowest Toxicity
Corrosive (irreversible destruction of
Corneal involvement or other eye ocular tissue) or corneal involvement
irritation clearing in 8-21 days or irritation persisting for more than
21 days
Severe irritation at 72 hours (severe Corrosive (tissue destruction into the
erythema or edema)
dermis and/or scarring)
Bleach;
Mikro Bac 3
Highest Toxicity
12
Quick Service Restaurant Validation
Efficacy validated in a real world setting
ATP
May 2013
96%
Reduction
APC
Sept. 2013
In Vitro
Oct. 2013
9 of 10
swabs
Out
performed
quaternary
ammonia
sanitizer
exceeded
success
target
APC
Jan. 2014
In Vitro
March 2014
96.4%
Greater
reduction in
established
biofilm
reduction in
microbial
events
APC
July 2014
99.7%
reduction in
total APC.
Superior
efficacy
maintained
for 60 days.
13
PURE Hard Surface On Board Ship Use
PURE® Hard Surface has been successfully
implemented for high-touch surfaces on-board ships in
the unnamed fleet.
Typical Use:
PURE® Hard Surface is ready-to-use and requires no dilution.
1.Pre-Clean: If surfaces are visibly soiled, pre-clean surfaces prior to using PURE
Hard Surface. You may use PURE Hard Surface as the pre-cleaning agent.
2.To kill viruses (such as Norovirus), apply, spray, or mist PURE Hard Surface to
the surface until wet for 60 seconds. The surface should then be wiped dry with a
clean towel.
3.To kill bacteria, apply, spray, or mist PURE® Hard Surface to the surface until
wet for 2 minutes. The surface should then be wiped dry with a clean towel.
Procedures may vary by target surfaces throughout the ship, i.e. food
contact and prep surfaces to stairway hand rails and elevator buttons.
Intercon Chemical will help develop specific protocols to achieve the
above application.
Required PPE
None required by OSHA or NIOSH
14
PURE Hard Surface: Broad Spectrum
The problem does not stop with Norovirus
Campylobacter
Enveloped and Non
Enveloped Viruses
Food Borne
Pathogens
Drug Resistant
Organisms
Influenza
Human Coronavirus
RSV & Rhinovirus
Rotavirus
Adenovirus
Ebola & EV-D68
Salmonella
Campylobacter
Escherichia coli (E. coli)
Listeria
MRSA
CA-MRSA
PVL- MRSA
VRE
CRE
PURE Hard Surface is the total solution to ship disinfection.
15
Protect your brand!
16
Contact Information
Tom Myers
[email protected]
PURE Bioscience, Inc.
VP Technical Services
1725 Gillespie Way
El Cajon, CA 92020
619-596-8600 Ext. 108
Jim Epstein
[email protected]
Intercon Chemical Company
President & CEO
1100 Central Industrial Drive
St. Louis, MO 63110
314-504-0119