Dynamics of cell-mediated aggregation
Cristóvão Dias
Centro de Física Teórica e Computacional, Universidade de Lisboa, Portugal
Departamento de Física, Faculdade de Ciências, Universidade de Lisboa, Portugal
Encontro CIÊNCIA 2017
Lisboa, Portugal
4/Julho/2017
Morphogenesis
Wound healing
Genetics 156, 1671 (2000)
3D models
http://www.biophysics.com/healingmodule.php
Regenerative Medicine
National Geographics
Andrew Pelling (http://www.pellinglab.net)
Scaffolds
3D printing
flat substrates
http://www.wakehealth.edu/
self-assembly
Soft Matter 9, 2985 (2013)
Biomaterials 43, 23 (2015)
Cell-mediated self-assembled scaffolds
C. A. Custódio, M. T. Cerqueira, A. P. Marques, R. L. Reis, and J. F. Mano, Biomaterials 43, 23 (2015)
Aggregation dynamics
particle-based simulations
Cell adheres to
the particle
•
n sites on the surface (n = 6);
•
one site only adheres to one cell;
•
cell-site interaction is attractive;
•
colloid-colloid interaction is repulsive.
cell-mediated
particle-particle
bond
Aggregation dynamics
particle-based simulations
Cell/colloid interaction
Cell/Cell-colloid interaction
Langevin Dynamics:
Stochastic force
drag term
inter-particle force
Aggregation dynamics
particle-based simulations (results)
512 particles
total time = 100
Dcell/Dparticle = 10
10 samples
Size of the
largest
aggregate
cell/site ratio
Aggregation dynamics
particle-based simulations (results)
512 particles
total time = 100
Dcell/Dparticle = 10
10 samples
Aggregation dynamics
Free cell
mean-field approximation
Cell-particle
Cell adheres to
the particle
Particle-cell-particle
cell-mediated
particle-particle
bond
Aggregation dynamics
mean-field approximation
512 particles
total time = 100
Dcell/Dparticle = 10
10 samples
Aggregation dynamics
mean-field approximation
k1/k0=1
k1/k0=10-1
5
5
k1/k0=10-2
Aggregation dynamics
lattice model (without loops)
Aggregation dynamics
lattice model (without loops)
Aggregation dynamics
lattice model (without loops)
Future…
Lattice model
Molecular dynamics
Aggregates
•
loops
•
diffusion coefficient
•
mechanical properties
Spatial correlations
•
competing timescales
•
aggregation dynamics
•
size distribution
Proliferation model
Cell dynamics
•
adhesion
•
chemotaxis
•
division
•
extrusion
Margarida Telo da
Gama
Nuno Araújo
Gonçalo Antunes
Universidade
de Aveiro
João Mano
Catarina Custódio
Financial support from the Portuguese Foundation for Science and Technology (FCT) under
Contracts nos. EXCL/FIS-NAN/0083/2012.