Caspase-1 Inflammasome Activation Mediates
Homocysteine-Induced Pyrop-Apoptosis in Endothelial
CellsNovelty and Significance
by Hang Xi, Yuling Zhang, Yanjie Xu, William Y. Yang, Xiaohua Jiang, Xiaojin Sha,
Xiaoshu Cheng, Jingfeng Wang, Xuebin Qin, Jun Yu, Yong Ji, Xiaofeng Yang, and
Hong Wang
Circulation Research
Volume 118(10):1525-1539
May 13, 2016
Copyright © American Heart Association, Inc. All rights reserved.
Homocysteine (Hcy) suppresses cell viability and induces pyroptosis/apoptosis in endothelial
cells.
Hang Xi et al. Circ Res. 2016;118:1525-1539
Copyright © American Heart Association, Inc. All rights reserved.
Homocysteine (Hcy) activates endothelial casp1 before casp8, 9, 3 activations in the
presence/absence of lipopolysaccharide (LPS).
Hang Xi et al. Circ Res. 2016;118:1525-1539
Copyright © American Heart Association, Inc. All rights reserved.
Caspase-1 mediates homocysteine (Hcy)-induced pyroptosis/apoptosis and casp9/3 activities in
the presence/absence of lipopolysaccharide (LPS).
Hang Xi et al. Circ Res. 2016;118:1525-1539
Copyright © American Heart Association, Inc. All rights reserved.
Homocysteine (Hcy) induces inflammasome assembly, leading to casp1 activation, and
interleukin (IL)-1β cleavage in the presence/absence of lipopolysaccharide (LPS).
Hang Xi et al. Circ Res. 2016;118:1525-1539
Copyright © American Heart Association, Inc. All rights reserved.
Homocysteine (Hcy)-derived reactive oxygen species trigger endothelial casp1 activation.
Hang Xi et al. Circ Res. 2016;118:1525-1539
Copyright © American Heart Association, Inc. All rights reserved.
Homocysteine (Hcy)/lipopolysaccharide (LPS)-induced intracellular reactive oxygen species
(ROS) levels determines cell destiny (pyroptosis/apoptosis/viable cell) in endothelial cells.
Hang Xi et al. Circ Res. 2016;118:1525-1539
Copyright © American Heart Association, Inc. All rights reserved.
Homocysteine (Hcy) induces mitochondrial membrane potential (Δψm) collapse and
cytochrome-c release, and increase Bcl-2–associated X protein (Bax)/B-cell lymphoma 2 (Bcl-2)
Ratio via oxidative stress, Casp1 activation in endothelial cells.
Hang Xi et al. Circ Res. 2016;118:1525-1539
Copyright © American Heart Association, Inc. All rights reserved.
Homocysteinemia (HHcy)-induced Casp1 activation mediates endothelial cell (EC) death in
mouse aortic endothelial cells (MAECs) and impaired endothelial-dependent vessel relaxation in
mouse aorta.
Hang Xi et al. Circ Res. 2016;118:1525-1539
Copyright © American Heart Association, Inc. All rights reserved.