Choose NEB DNA Ligases: for quality, performance & convenience With over 40 years of experience in the development and production of enzymes for molecular biology, NEB offers the most extensive selection of high-quality, and performance- optimized DNA ligases and ligase master mixes. NEB ligases and ligase master mixes are manufactured to the highest level of purity in the industry, and then rigorously tested for optimal performance. NEB’s T4 DNA Ligase has been referenced in peerreviewed publications for decades, and is renowned for its consistent quality. • Industry standard for purity • Highest reliability • Fast ligations • Robust ligation efficiency • Convenient master mix formats Take advantage of our promotions! Q5 High-Fidelity DNA Polymerase: Ultra-fidelity PCR in space & on earth! Genes in Space competition's winning experiment conducted aboard the International Space Station using Q5 HiFi DNA Polymerase. Watch NEB TV Episode 10 to learn more about the first PCR in space: www.neb-online.eu/neb-tv-10 Q5 High-Fidelity DNA Polymerase is an ultra-fidelity PCR polymerase. With a fidelity >100-fold higher compared to Taq DNA Polymerase and 12-fold higher than Pyrococcus furiosus (Pfu) DNA Polymerase, Q5 High-Fidelity DNA Polymerase is ideal for cloning (virtually “error-free”). It also can be used for long or difficult amplicons, as it is fused to a processivity-enhancing protein domain that supports robust DNA amplification. Q5 High-Fidelity DNA Polymerase is the first choice for all high-fidelity PCRs on earth as well as in space! • Fidelity – the highest fidelity amplification available (> 100x higher than Taq and 2x higher than Phusion) • Robustness – high specificity and yield with minimal optimization • Coverage – superior performance for a broad range of amplicons (from high AT to high GC) • Speed – short extension times • Amplicon length – robust amplifications up to 20 kb for simple templates, and 10 kb for complex Test now & benefit from a special introductory price: OneTaq Quick-Load DNA Polymerase NEB’s new OneTaq Quick-Load DNA Polymerase is an optimized, ready-to-use blend of Taq and Deep VentR DNA Polymerases ideally suited to “standard” PCR applications from a variety of templates, including pure DNA solutions, bacterial colonies, cDNA products etc. It is supplied with a density and tracking dye containing 5x OneTaq Quick-Load Reaction Buffer for direct gel loading in addition to the regular “colorless” 5x OneTaq Reaction Buffer. • High and robust yields with minimal optimization making it the first choice for daily „Standard“-PCRs • Includes „colored“ Quick-Load reaction buffer that allows for direct gel-loading • Value pricing Take advantage of our promotions! New England Biolabs Promotions: Cat. No. Description DNA Ligases M0202S T4 DNA Ligase M0202T T4 DNA Ligase, conc. M0202L T4 DNA Ligase M0202M T4 DNA Ligase, conc. M2200S Quick Ligation™ Kit M2200L Quick Ligation™ Kit M0367S Blunt/TA Ligase Master Mix M0367L Blunt/TA Ligase Master Mix M0370S Instant Sticky-end Ligase Master Mix M0370L Instant Sticky-end Ligase Master Mix ® Q5 High-Fidelity DNA Polymerase ® M0491S Q5 High-Fidelity DNA Polymerase ® M0491L Q5 High-Fidelity DNA Polymerase ® M0493S Q5 Hot Start High-Fidelity DNA Polymerase ® M0493L Q5 Hot Start High-Fidelity DNA Polymerase ® M0492S Q5 High-Fidelity 2X Master Mix ® M0492L Q5 High-Fidelity 2X Master Mix ® M0494S Q5 Hot Start High-Fidelity 2X Master Mix ® M0494L Q5 Hot Start High-Fidelity 2X Master Mix ® E0554S Q5 Site-Directed Mutagenesis Kit ® E0552S Q5 Site-Directed Mutagenesis Kit (Without Competent Cells) ® E0555S Q5 High-Fidelity PCR Kit ® E0555L Q5 High-Fidelity PCR Kit ® ® OneTaq Quick-Load DNA Polymerase ® ® M0509S OneTaq Quick-Load DNA Polymerase ® ® M0509L OneTaq Quick-Load DNA Polymerase ® ® M0509X OneTaq Quick-Load DNA Polymerase Size List Price CHF % Offer Price CHF 20`000 units 20`000 units 100`000 units 100`000 units 30 assays 150 assays 50 reactions 250 reactions 50 reactions 250 reactions 104,00 104,00 416,00 416,00 151,00 606,00 150,00 600,00 150,00 600,00 20% 20% 20% 20% 20% 20% 20% 20% 20% 20% 83,20 83,20 332,80 332,80 120,80 484,80 120,00 480,00 120,00 480,00 100 units 500 units 100 units 500 units 100 reactions 500 reactions 100 reactions 500 reactions 10 reactions 10 reactions 50 reactions 200 reactions 141,00 627,00 165,00 750,00 230,00 918,00 268,00 1`071,00 282,00 193,00 138,00 467,00 20% 20% 20% 20% 20% 20% 20% 20% 20% 20% 20% 20% 112,80 501,60 132,00 600,00 184,00 734,40 214,40 856,80 225,60 154,40 110,40 373,60 100 units 500 units 2`500 units 31,00 122,00 574,00 25% 25% 25% 23,00 92,00 431,00 This offer is valid until 31.01.2017. No other discounts apply. All prices in CHF excl. VAT. Please mention promotion code NEB0117 when ordering.
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