HTSF Instrumentation for Users
®
EnVision Multilabel Plate Reader
Model 2104-0020
Technologies
The system reads all non-isotopic detection technologies in all major applications, including:
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Top and Bottom Read of Fluorescence
Luminescence
Absorbance
Applications
EnVision is a multilabel reader for all major applications, including
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Reporter gene assays
Cell applications
Genotyping
Enzyme assays
Signal transduction
Receptor ligand binding
Immunoassays
Quantification
Possible other future assays.
Counting modes and features included
EnVision counting modes are easily accessible. In the same protocol, even for the same well, you can freely use
several different counting modes and labels.
Plate format:
96-well; 384-well; 1536-well
Temperature Control
To ensure reproducibility of cellular and enzyme kinetic assays and other assays that require a defined temperature
range, the EnVision platform’s temperature control option lets you precisely regulate from +2°C to +45°C above
ambient. Temperature control is uniform across the plate, and a top heating plate eliminates condensation problems.
Dispenser
The EnVision dispenser can be used with 1- to 384-well plates in all reading modes. It consists of two pump units, a
magnetic stirrer and a heater, allowing precise delivery of reagents in 1 μL increments over a volume range of 2-475
μL. An adjustable dispense speed setting lets you optimize for viscosity, cell density and other assay requirements.
Plate Stacker
Available for 20 or 50 plates, interchangeable plate stacker magazines can easily be added to increase capacity and
throughput. For kinetic assays, the grip-fed re-stacking function lets you maintain the order of plates in the stack,
increasing the reliability of data.
Plate barcode
The barcode reader allows you to load barcode labeled plates, which are identified by the barcode reader. Codabar,
Code39, Interleaved 2 of 5, Code 128, UPC and EAN barcodes can be read.
Plate loading modes
Available in HTSF:
Manual reading (one plate at time)
Stacker loading (up to 50 plates per load)
Optical filters
A wide range of filters cover the wavelengths used in fluorometric and photometric applications. Below are 16 filters
available in HTSF:
Barcode
Wavelength
Barcode
Wavelength
217
M590
103
X355
243
M685
318
P570
229
M595w
105
X531
214
M510
302
P405
207
M460
227
M650
206
M535
112
X380
102
X485
118
X620
319
P600
303
P450
Application-specific mirror modules
User interchangeable application specific optical modules form a crucial part of the optical system. The optical
modules include all application critical components; for example dichroicmirrors for specific labels. All applicationspecific optical modules are barcoded.
Top and bottom reading
Fluorescence readings (including FP and TRF) can be taken from the top or bottom. Top reading is the most efficient
method when no lid is used because no plastic surface has to be penetrated. For adherent cells and lidded plates,
below reading provides superior efficiency. It is a true epimode, in the sense that the both excitation and emission are
directed to and from below. For top reading, four optical modules can be in place. Any of the them can also be used
for below reading.
Below are 5 mirror modules available in HTSF.
Barcode
Wavelength?
401
B550
403
D505
405
D555
412
D400
420
D658
Ultra-Sensitive Luminescence technology makes the most of your precious cells and reagents
For the ultra sensitive luminescence, we have 5 types apertures (96-L1, 384-L1,1536-L1,1536-LP-L1 and 0L1/A1) for
96, 384 ad 1536 plates respectively. They are working with primary, stem or difficult-to-transfect cells presents a
unique set of challenges. Optional ultra- sensitive luminescence boosts sensitivity by almost 40X vs. standard
luminescence by bringing the detector closer to the sample—giving you more information from every cell.
Software that enhances both productivity and compliance
Powerful software makes it easy to take advantage of all the performance and versatility of the EnVision platform. An
intuitive interface with pre-set user-specific protocols makes setting up the system fast and easy. For post-run data
analysis, features such as automatic curve fitting and flexible interpretation of standards controls and unknowns gives
the EnVision system the power to handle a broad range of assay types. The software integrates smoothly into your
lab’s network and into lab automation and data management systems. For regulated environments, PerkinElmer’s
Enhanced Security mode provides technological controls and features that support 21 CFR Part 11 compliance.
Visit www.perkinelmer.com/EnVision for more information.