COA A No: CA BMM M‐0007 Certifiicate of An nalysis Verrsion: 01 BIO‐X X‐ACT™ ™ Short Mixx For Reseaarch Use Only Storaage Conditio ons: ‐20 0°C Lot n number: BXSX‐3 313211 Expirry date: Novemb ber 2015 Quality CControl Pa arameterss Analysis Specificcation Result Fun nctional A 2.4Kb b fragment iss amplified w with a dilution series of h uman genomiic DNA, usingg standard conditions an nd 30 cycles. Single distinctt bands weree observed w with agarose gel electrop horesis (ethidiu um stained). Passed DN NA contaminaation Quantittative PCR annalysis with n no template. Presence oof E. coli and Passed mouse genomic DN NA checked. Test sample must ampliffy in line with a refere ence sample . DN Nase contamiination Incubattion of a 1Kbb double stranded DNA frragment. Inccubation for 4 hourss at 37°C withh dilution series of DNase I. Analysedd by agarose e gel elecctrophoresis.. Test samplle must show w less degraddation than the limit of detectioon 2.5 x 10‐3 U DNase. Passed Auth horised by Jaade James Euro ope Head dquarters UK Europe Germany Ameriica Australia info.u [email protected] m [email protected] [email protected] [email protected] Tel: +44 + (0)20 8830 5300 Fax: +44 (0)20 8452 2 2822 Tel: +49 (0)33 371 681 229 Fax: +49 (0)3 3371 681 244 Tel: +1 1 508 880 8990 Fax: +1 + 508 880 8993 3 Tel: +61 (0)2 9209 9 4180 Fax: +61 (0)2 9209 4763 COA A No: CA BB‐00 014 Certifiicate of An nalysis Verrsion: 01 MgCll2 Solu ution, 5 50mM For Reseaarch Use Only Storaage Conditio ons: ‐20 0°C Lot n number: MG‐113111 Expirry date: Novemb ber 2015 Quality CControl Pa arameterss Analysis Specificcation Result Fun nctional Fragme ents of sizes 8800bp and 3 3000bp are amplified wit h a dilution series o of BIOTAQTM DNA Polyme erase, using standard connditions and 30 cycle es. Single disstinct bands were observved with agaarose gel electrophoresis (ethhidium stained). Passed DN NA contaminaation Quantittative PCR annalysis with n no template. Presence oof E. coli and Passed mouse genomic DN NA checked. Test sample must ampliffy in line with a refere ence sample . DN Nase contamiination Incubattion of a 1Kbb double stranded DNA frragment. Inccubation for 4 hourss at 37°C withh dilution series of DNase I. Analysedd by agarose e gel elecctrophoresis.. Test samplle must show w less degraddation than the limit of detectioon 2.5 x 10‐3 U DNase. Passed Auth horised by Jaade James Euro ope Head dquarters UK Europe Germany Ameriica Australia info.u [email protected] m [email protected] [email protected] [email protected] Tel: +44 + (0)20 8830 5300 Fax: +44 (0)20 8452 2 2822 Tel: +49 (0)33 371 681 229 Fax: +49 (0)3 3371 681 244 Tel: +1 1 508 880 8990 Fax: +1 + 508 880 8993 3 Tel: +61 (0)2 9209 9 4180 Fax: +61 (0)2 9209 4763
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