A
Ctr
PcDNA3.1+Ad.vec
CCN1
40.00%
PcDNA3.1-CCN1+Ad.vec
Sari
35.00%
PcDNA3.1+Ad.SARI
CCN1+sari
30.00%
PcDNA3.1-CCN1+Ad.SARI
45.00%
45
25.00%
40.00%
40
20.00%
35.00%
35
15.00%
30.00%
30
10.00%
25.00%
25
5.00%
0.00%
20
20.00%
15
15.00%
PHFA(Im) H4
U87
THR
10.00%
10
5.00%5
0.00%0
WM 238
MeWo
WM35
WM35
WM238
MeWo
45.00%
20
18
16.00%
16
14.00%
14
12.00%
12
10.00%
10
8.00%8
6.00%6
4.00%
4
2.00%
2
0.00%0
Cloning efficiency (%)
20.00%
Cloning efficiency (%)
18.00%
FM516-SV
FM516
B
0
12
24
PHFA-Im
PHFA(Im)
48
SARI
FM516-SV
CCN1
EF1α
pcDNA3.1-CCN1+Ad.vec
WM35
SARI
CCN1
EF1α
WM238
SARI
CCN1
EF1α
SARI
HO-1
CCN1
EF1α
Supplementary Figure S1
H4
H4
U87 MG
U87
THR
THR
Anti c-Jun
TPA
-
-
-
Anti c-Jun
SARI
+
-
+
SARI
-
-
+
+
+
-
+
+
+
+
TPA
-
+
-
+
CC
A
B
AP-1 supershift
SP-1 shift
AP-1 shift
WM238
WM238
Supplementary Figure S2