Bio Basic Inc.
A world leader in serving science
NEXT GENERATION SEQUENCING ORDER FORM
(DNA SAMPLES)
1. BASIC INFORMATION
NAME OF COMPANY
CONTACT PERSON
TELEPHONE NUMBER
EMAIL ADDRESS
ADDRESS
LAB NAME
GROUP / P.I.
RESEARCH OBJECTIVE
2. EXPERIMENTAL INFORMATION
BIORESEARCH FIELD
Genome
Transcriptome Level
Epigenetics
Other (Please specify below)
Other:
EXPERIMENTAL DESIGN
De Novo Sequencing
Whole Genome Re-sequencing
Whole Genome Bisulfite Sequencing
Genome Survey
Exome Sequencing
ChIP Sequencing
Metagonomic Sequencing
Target Region Sequencing
Genotyping
MeDIP Sequencing
Other:
20 Konrad Cres, Markham Ontario L3R 8T4 Canada
Tel: (905) 474 4493, (800) 313 7224 Fax: (905) 474 5794
Email: [email protected] Web: www.biobasic.com
Bio Basic Inc.
A world leader in serving science
3. SAMPLE INFORMATION
TYPE OF SAMPLE
Genomic DNA
PCR Products
Broken Short Fragments
ChIP-Seq DNA Samples
Other (Please Specify Below)
Other:
STATE OF SAMPLE
Dissolved in Water
Dissolved in TE Buffer
Dissolved in Pure Ethanol
Other (Please Specify Below)
Other:
4. SAMPLE DETAILS
Sample Name
Species
# of Tubes
Concentration (ng/µl)
Volume (µl)
Please send the completed form to: [email protected]
SAMPLE CONDITION: DNA Samples without degradation or RNA contamination
SAMPLE QUANITITY: m≥6µg
SAMPLE CONCENTRATION: 50 ng/µl
SAMPLE PURITY: OD 260/280 = 1.8 ~ 2.0
20 Konrad Cres, Markham Ontario L3R 8T4 Canada
Tel: (905) 474 4493, (800) 313 7224 Fax: (905) 474 5794
Email: [email protected] Web: www.biobasic.com
Total Quantity (µg)
Fragment Size
Bio Basic Inc.
A world leader in serving science
5. SAMPLE REQUIREMENTS
Library Types
Sample Types
Quantitative
Methods
Quantitative Result
Qubit
Sample With Single Band or Main
Apparent Band in Electrophoresis
(genome, plasmid, PCR product)
Qubit
m≥6µg
c≥30µg
Sample for Meta-genomics Sequencing
Qubit
m≥4µg
c≥30µg
Samples With Smear in Electrophoresis:
Not Fragmented
Qubit
m≥3µg
c≥30µg
Samples With Smear in Electrophoresis:
Fragmented
Qubit
m≥4µg
c≥30µg
2K Insert
Genomic DNA
Qubit
m≥40µg
c≥133µg
5-6K Insert
Genomic DNA
Qubit
m≥40µg
c≥133µg
10K Insert
Genomic DNA
Qubit
m≥60µg
c≥133µg
20/40K Insert
Genomic DNA
Qubit
m≥120µg
c≥133µg
Genomic DNA
Qubit
m≥20µg
c≥30µg
PCR Product with Single Band
Qubit
m≥6µg
c≥30µg
ChIP-Seq
ChIP-Seq DNA Samples
Qubit, Agilent 2100
MeDIP/Bisulfite
Genomic DNA
Qubit
EXON Agilent, Nimblegen
EZ, Nimblegen Array
(pooling)
Genomic DNA
EXON Nimblegen Array
(one sample for
Hybridization)
Genomic DNA
Genotyping
Genomic DNA
≤800 bp Insert
PCR Free
m≥20µg
m≥10µg
c≥50µg
m≥6µg
c≥50µg
Qubi
m≥30µg
c≥50µg
Qubit,
m≥2µg
c≥50µg
Qubit
20 Konrad Cres, Markham Ontario L3R 8T4 Canada
Tel: (905) 474 4493, (800) 313 7224 Fax: (905) 474 5794
Email: [email protected] Web: www.biobasic.com
Bio Basic Inc.
A world leader in serving science
6. Terms and Conditions
Terms
Bio Basic Inc. will send electronically DNA sequencing results to customers. All results will be
kept in Bio Basic Inc.’s data base for 6 months. During this period, customer may request
sequencing results. After 6 months, DNA sequencing results are automatically destroyed without
further notification.
DNA sequencing tests are not considered confirmed without the customer providing a P.O.
number or credit card number. Bio Basic Inc. accepts credit card (Visa or MasterCard), cheque, or
wire for payment.
Cancellations
Buyer may not cancel any order or return any samples without Bio Basic Inc.’s consent.
Cancellation and return charges may be charged. Buyer must contact Bio Basic Inc. to obtain a
return material authorization number.
Warranty
Bio Basic Inc. does not guarantee any downstream application(s) or usage of the DNA sequencing
results. Any claims or dispute must be submitted within 2 weeks of the results being sent. At the
time of shipment, Bio Basic Inc. keeps a database for unexpected incidents and/or disputes. Bio
Basic Inc. reserves the right to refuse handling disputes after a period of 3 months.
Prices
All prices are subject to correction of errors; Bio Basic Inc. reserves the right to adjust prices on
orders during production due to changes in cost of materials, transportation or wages. Any tax,
customs, surcharge or duty, howsoever denominated, imposed upon the sale, importation,
delivery or use of products shall be the responsibility of the Buyer.
20 Konrad Cres, Markham Ontario L3R 8T4 Canada
Tel: (905) 474 4493, (800) 313 7224 Fax: (905) 474 5794
Email: [email protected] Web: www.biobasic.com