Table S2. Gene expression and steatosis incidence in a dose response to Tm.
Larvae were treated on 3 dpf with DMSO (control) or Tm concentrations ranging from
0.005 to 1 µg/ml. Total number of larvae scored, steatosis incidence (i.e. percent) and the
Ct for UPR target genes measured by qPCR are shown for each clutch.
Drug
(mg/ml)
DMSO
TM 0.005
TM 0.01
TM 0.03
TM 0.06
Clutch
#
# of
Larvae w/
steatosis
Gene expression (2^-Ct target-2^-Ct rppo)
Total #
larvae
% steatosis
dnajc3
edem1
ddit3
xbp1s
6.66
0.00414
0.001044
0.004158
0.0054861
0.00124757
0.003369
0.0005888
bip
atf4
atf6
DR1
1
15
DR2
0
14
0
0.01643
0.002208
0.003247
0.0055370
0.00366154
0.000898
0.0026866
DR3
2
12
16.66
0.00666
0.001944
0.002078
0.0061579
0.00241014
0.000788
0.0012886
DR4
3
15
20
0.01431
0.004044
0.002167
0.0030087
0.00174004
0.001268
0.0011885
DR5
7
23
30.43
0.002031
0.0025891
DR6
6
18
33.33
0.002595
0.00186062
DR7
0
9
0
DR8
5
12
41.66
0.00338
0.001962
0.002559
0.0043442
0.00435433
0.002730
0.0011089
DR9
3
11
27.27
0.01679
0.002906
0.003998
0.0043243
0.00296723
0.001313
0.0002551
DR10
8
15
53.33
0.00108
0.000956
0.002223
0.0029399
0.00222291
0.000784
0.0012505
DR11
1
11
9.1
0.008628
0.00437449
0.00259508
0.006599931
0.004581387
0.00372985
0.004764917
DR12
1
14
7.14
0.031906
0.00406273
0.00428447
0.006316466
0.004006801
0.00284635
0.006896117
DR13
2
16
12.5
0.005274
0.00522628
0.00293315
0.005398097
0.003136418
0.00278778
0.005486113
DR14
1
13
7.69
0.010003
0.00402535
0.00236600
0.004842608
0.003721242
0.00198497
0.00536081
DR15
1
16
6.25
0.002074
0.00365309
0.00110889
0.004148101
0.003636249
0.00366154
0.004100456
DR7
4
12
33.33
DR8
3
10
30
0.00440
0.003906
0.002988
0.0060732
0.00466686
0.002583
0.0026619
DR9
1
13
7.69
0.05913
0.007074
0.005907
0.0065847
0.00788504
0.003300
0.0117597
DR10
7
15
46.66
0.03436
0.003277
0.003730
0.0101199
0.00573231
0.004385
0.0046026
DR7
4
13
30.77
DR8
3
13
23.08
0.00658
0.002377
0.002724
0.0090995
0.00476492
0.004776
0.0034720
DR9
1
13
7.69
0.08697
0.007705
0.007831
0.0115711
0.00828662
0.004721
0.0142128
DR10
8
16
50
0.05777
0.004798
0.0027878
0.00806934
0.007008558
0.0044664
0.00723897
DR11
2
11
18.18
DR1
8
16
50
0.13934
0.009663
0.0104284
0.01030866
0.00596195
0.0025125
0.00413853
DR2
2
17
11.76
DR3
10
33
30.30
0.05329
0.005013
0.0038882
0.00705731
0.006229505
0.0029264
0.00412898
DR4
14
18
77.78
0.01038
0.0019531
0.00248938
0.0019758
0.00416731
DR5
10
20
50
0.00857
0.0027304
0.00525049
0.0047539
0.00274940
DR6
4
19
21.05
DR7
5
12
41.67
DR8
7
14
50
0.006144
0.003496
0.00171212
0.003628
0.004324253
0.004944362
0.01326
0.003679
0.0038436
0.01069688
0.008032139
0.0056534
0.00786684
DR9
2
13
15.38
0.08839
0.007903
0.0061437
0.00850976
0.007222263
0.0044767
0.01175974
DR10
12
15
80
0.04781
0.003730
0.0020885
0.01019025
0.008181985
0.0050951
0.00950784
DR1
14
16
87.5
0.03777
0.004177
0.0043543
0.01120277
0.004539241
0.0024722
0.00287279
DR2
8
14
57.14
DR3
14
33
42.42
0.05147
0.004167
0.0033152
0.01074642
0.008050719
0.0047649
0.00546082
DR4
9
20
45
0.02916
0.007222
0.0033849
0.00472108
0.0082771
0.00440493
DR6
9
20
45
DR8
10
14
71.43
0.0071393
0.00723897
0.006801176
0.03821
0.006896
0.0037212
0.01033250
0.007511586
TM 0.12
TM 0.25
TM 0.5
DR7
9
13
69.23
DR1
15
17
88.24
DR2
7
14
50
DR3
9
29
DR4
9
20
DR8
12
DR7
7
DR9
DR10
0.01833
0.002693
0.0052626
0.01295812
0.004384617
0.0049903
0.00221266
31.03
0.01379
0.003611
0.0021225
0.00554986
0.004591984
0.0037041
0.00266805
45
0.03708
0.005072
0.0031949
0.00492157
0.0049330
0.00720560
15
80
0.04961
0.008163
0.0034085
0.01897180
0.01674646
0.0109976
0.00993453
11
63.64
6
15
40
0.0625
0.00669
0.0073740
0.01026113
0.00905753
0.0082580
0.01505792
15
15
100
0.01308
0.00847
0.0009743
0.00216213
0.001760255
0.0063604
0.01197913
DR2
9
17
52.94
0.09785
0.006554
0.0043846
0.01901568
0.01257469
0.0137922
0.00977514
DR4
15
20
75
0.11370
0.008014
0.0056273
0.00831539
0.01014327
0.0179899
0.00998054
DR8
11
12
91.67
0.05354
0.009334
0.0072893
0.02100194
0.019823195
0.013261
0.01038036
76.92
DR7
10
13
DR11
11
11
100
0.059815
0.01135916
0.00922650
0.0078125
0.01248783
0.00825795
0.012459011
DR12
9
14
64.28
0.111619
0.01002676
0.00905753
0.00905753
0.00779447
0.00543564
0.013888167
DR13
12
12
100
0.130911
0.02038050
0.01125466
0.010428436
0.016630784
0.00570588
0.025796754
DR14
7
12
58.33
0.031759
0.00317286
0.00447674
0.006229505
0.004570813
0.00348812
0.008490116
DR15
8
10
80
0.039463
0.01283891
0.00388824
0.005238369
0.00837323
0.0078125
0.015057924
0.10757
0.009574
0.0049673
0.02090512
0.011023045
0.0085098
0.00946401
0.08401
0.012007
0.0055499
0.00612956
0.010549609
0.0101433
0.00691207
0.07802
0.009163
0.0112028
0.02324934
0.01946015
0.0079033
0.01498850
0.0091205
0.00633108
DR1
DR2
15
19
78.95
DR4
18
20
90
DR8
9
11
81.82
DR3
TM 1.0
DR1
DR2
0.007564
14
15
93.33
0.11690
DR3
0.010003625
0.0045497
0.01682403
0.023089
0.013384125
DR4
19
19
100
0.08362
0.012517
0.0068643
0.00850976
0.010119861
0.0119515
0.00866851
DR8
7
11
63.64
0.05280
0.008830
0.0122026
0.04085529
0.021393752
0.0129282
0.01175974
DR7
9
12
75
DR9
0.020617
0.012090352
DR10
0.024349
0.007041019