ProxiMAX randomization: a new technology for nondegenerate saturation mutagenesis of contiguous codons
by Mohammed Ashraf, Laura Frigotto, Matthew E. Smith, Seema Patel, Marcus D.
Hughes, Andrew J. Poole, Husam R.M. Hebaishi, Christopher G. Ullman, and
Anna V. Hine
Biochm. Soc. Trans.
Volume 41(5):1189-1194
October 1, 2013
©2013 by Portland Press Ltd
Comparison of performance of common saturation mutagenesis techniques Green coloration
indicates ideal performance, pale pink coloration indicates tolerable performance, and deep pink
coloration indicates unacceptable performance, where non-degenerate metho...
Mohammed Ashraf et al. Biochm. Soc. Trans. 2013;41:11891194
©2013 by Portland Press Ltd
ProxiMAX methodology Double-stranded DNA donors, carrying the required ‘MAX’ codons at
their termini, are ligated individually on to a double-stranded DNA acceptor sequence
(phosphorylated at the required 5′ end only).
Mohammed Ashraf et al. Biochm. Soc. Trans. 2013;41:11891194
©2013 by Portland Press Ltd
Library construction with controlled codon ratios (A) DNA sequence analysis of a library
encoded by six cycles of codon addition, optimized to provide unbiased representation.
Mohammed Ashraf et al. Biochm. Soc. Trans. 2013;41:11891194
©2013 by Portland Press Ltd