A
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D
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H
S4. (A) Western blots for PARP1, LIG3 and c-MYC proteins in (A) MOLM14 cells treated with c-MYC inhibitor 10058-F4 and (B)
siRNA depletion of c-MYC in MO7e-BCR-ABL1 cells, in experiments for NHEJ assays. (C) Flow cytometric data for GFP in
MOLM14 cells transfected with pmaxGFP vector treated with DMSO (left panel) and 30uM c-myc inhibitor 10058-F (right panel).
The inset numbers (DMSO= 81.4 and 10058-F4=78.7) denote the percentage of GFP-positive cells. (D), Average colony numbers
from end-joining repair experiments in siRNA c-MYC, control and linear pUC18. (E), fraction of small and large deletions in siRNA
c-MYC and controls. (F) NHEJ assay using HindIII-linearized pEGFP-Pem1-Ad2 vector in MOLM14 analyzed by flow cytometry.
The graph represents three independent experiments. The p-values above each column were obtained by t-tests comparing each
group to cells transfected with siCtrl. (G) Western blot analysis of LIG4, Ku70, c-MYC in MOLM14 cells. (H), Western blot analysis