Decreased expression of let-7c is associated with non-response of
muscle-invasive
bladder
cancer
patients
to
neoadjuvant
chemotherapy – Vinall et al
Supplementary Figure 1: Receiver operating curve (ROC) plot for classifier based on let-7c. The
area under the ROC curve is 0.72. The level of let-7c at which the predicted probability of response is
50% is 6.2. Thirteen out of 22 patients (59%) with let-7c levels above this cutoff responded to
neoadjuvant chemotherapy, compared to 7 out of 18 (39%) patients with let-7c levels below this
cutoff. For a cutoff of 6.2, the sensitivity of the classifier is 65% and the specificity is 55%.
1
Supplementary Table 1: Comparative analysis of the most highly differentiated miRNA in tissues from
MIBC patients who were responders vs non-responders to chemotherapy. Highlighted in grey are the ones that
were reanalyzed by qPCR.
SystematicName
hsa-miR-24
hsa-miR-214
hsa-miR-193a-3p
hsa-let-7a
hsa-miR-15a
hsa-let-7g
hsa-let-7c
hsa-miR-130a
hsa-let-7f
hsa-miR-106b
hsa-miR-23a
hsa-miR-15b
hsa-miR-210
hsa-miR-21
hsa-miR-107
hsa-miR-29b
hsa-miR-199a-3p
hsa-let-7d
hsa-miR-424
hsa-miR-200a
hsa-let-7e
hsa-miR-96
hsa-miR-27a
hsa-miR-26b
hsa-miR-183
hsa-miR-301a
hsa-miR-26a
hsa-miR-103
hsa-miR-27b
hsa-miR-23b
hsa-miR-20b
hsa-miR-93
hsa-let-7i
hsa-miR-20a
hsa-let-7b
hsa-miR-363
hsa-miR-199b-5p
hsa-miR-28-5p
hsa-miR-148a
hsa-miR-17
hsa-miR-425
hsa-miR-768-3p_v11.0
hsa-miR-16
hsa-miR-199a-5p
Fold change([responders] vs
[Non-responders])
5.1154265
4.57229
4.4110894
4.208447
4.091291
3.8028967
3.746715
3.6430833
3.6333504
3.5025506
3.4677122
3.446
3.3543355
3.2745962
3.2666154
3.260668
3.2600694
3.2064848
3.2038114
3.1746733
3.1038437
3.0804327
3.0731142
3.070323
3.0436797
2.9929452
2.9640954
2.956494
2.8818622
2.8748837
2.866379
2.8621688
2.8432555
2.8358867
2.8347828
2.7746406
2.7697704
2.7475243
2.7208924
2.6948256
2.6940055
2.672554
2.6195066
2.5988815
2
Regulation([Yes] vs
[No])
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hsa-miR-203
hsa-miR-22
hsa-miR-151-5p
hsa-miR-29a
hsa-miR-98
hsa-miR-200c
hsa-miR-19b
hsa-miR-455-3p
hsa-miR-101
hsa-miR-331-3p
hsa-miR-34a
hsa-miR-141
hsa-miR-652
hsa-miR-429
hsa-miR-30b
hsa-miR-195
hsa-miR-361-5p
hsa-miR-148b
hsa-miR-140-5p
hsa-miR-454
hsa-miR-99a
hsa-miR-361-3p
hsa-miR-140-3p
hsa-miR-10a
hsa-miR-31*
hsa-miR-374a
hsa-miR-126
hsa-miR-324-5p
hsa-miR-29c
hsa-miR-130b
hsa-miR-142-3p
hsa-miR-128
hsa-miR-25
hsa-miR-7
hsa-miR-192
hsa-miR-451
hsa-miR-196a
hsa-miR-193b
hsa-miR-200b
hsa-miR-222
hsa-miR-19a
hsa-miR-30e*
hsa-miR-551b
hsa-miR-146b-5p
hsa-miR-497
hsa-miR-30c
hsa-miR-185
hsa-miR-34b*
hsa-miR-376c
hsa-miR-375
hsa-miR-95
hsa-miR-125b
2.591991
2.5900683
2.568789
2.563271
2.5448046
2.514143
2.508034
2.494452
2.4267457
2.4092984
2.395752
2.3697953
2.3314195
2.2713223
2.208189
2.2075374
2.1945052
2.1562998
2.1513982
2.1300707
2.1079018
2.045605
2.024905
2.0189683
1.9785365
1.9682323
1.9664462
1.9492303
1.9033198
1.8911893
1.889597
1.8813537
1.8679994
1.8595637
1.8438987
1.8307053
1.8300179
1.8177115
1.8064072
1.806194
1.8035554
1.8034444
1.7937772
1.7856083
1.7750026
1.7561895
1.7146778
1.7127903
1.7064095
1.6957567
1.6741487
1.6590105
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hsa-miR-662
hsa-miR-221
hsa-miR-374b
hsa-miR-200b*
hsa-miR-223
hsa-miR-196b
hsa-miR-150
hsa-miR-152
hsa-miR-431
hsa-miR-143
hsa-miR-10b
hsa-miR-132
hsa-miR-801_v10.1
hsa-miR-602
ebv-miR-BART12
hsa-miR-296-3p
hsa-miR-18b
hsa-miR-191*
hsa-miR-34c-5p
kshv-miR-K12-9*
hsa-miR-500
hsa-miR-636
hsa-miR-648
hsa-miR-320a
hsa-miR-885-5p
hsa-miR-1238
kshv-miR-K12-3
hsa-miR-512-3p
hsa-miR-877
hsa-miR-887
hsa-miR-615-3p
hsa-miR-1236
hsa-miR-940
hsa-miR-155
hsa-miR-1234
hsa-miR-181d
hsa-miR-617
hsa-miR-133a
hsa-miR-202
hsa-miR-936
hsa-miR-486-5p
hsa-miR-638
hsa-miR-769-3p
hsa-miR-525-5p
hsa-miR-139-3p
hsa-miR-874
hsa-miR-1225-3p
hsa-miR-10b*
hsa-miR-596
hcmv-miR-US4
hsa-miR-760
hsa-miR-625*
1.6583761
1.6280845
1.6140921
1.6059076
1.5826814
1.5629684
1.554363
1.5507895
1.5371733
1.5202504
1.5132589
1.5120989
1.5022966
1.5121675
1.5232949
1.5364202
1.5446181
1.5527499
1.5550215
1.5652902
1.5922074
1.5987458
1.6061159
1.6291193
1.6384997
1.639346
1.639351
1.6497953
1.6551211
1.658529
1.6708504
1.6722618
1.6756939
1.6895447
1.6999598
1.7044969
1.7211885
1.7223527
1.7258105
1.7449207
1.7501721
1.7539074
1.7564
1.7644101
1.7741019
1.7757512
1.8155075
1.8201276
1.8297071
1.8307956
1.8434393
1.8963245
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hsa-miR-345
hsa-miR-572
hsa-miR-575
kshv-miR-K12-8
hsa-miR-518a-5p
hsa-miR-1237
hsa-miR-328
hcmv-miR-UL70-3p
hsa-miR-18b*
hsa-miR-498
hsa-miR-371-5p
hsa-miR-125b-1*
hsa-miR-28-3p
hsa-miR-630
hsa-miR-550
hsa-miR-490-5p
hsa-miR-574-5p
hsa-miR-584
hsa-miR-194*
hcmv-miR-UL148D
hsa-miR-939
hsa-miR-632
hsa-miR-206
hsa-miR-125b-2*
hsa-miR-518e*
miRNABrightCorner30
hsa-miR-610
hsa-miR-1224-5p
hsa-miR-708
hsa-miR-1225-5p
hsa-miR-518b
hsa-miR-622
hsv1-miR-LAT_v10.1
ebv-miR-BART13
hsa-miR-1228
hsa-miR-373*
hsa-miR-1228*
hsa-miR-30b*
hsa-miR-193b*
hsa-miR-302c*
hsa-miR-663
hsa-miR-639
hsa-miR-296-5p
hsa-miR-150*
hsa-miR-566
ebv-miR-BART7
hsa-miR-125a-3p
hsa-miR-583
hsa-miR-338-5p
hcmv-miR-US25-1
hsa-miR-483-5p
hsa-let-7d*
1.9095659
1.9192595
1.9241668
1.931153
1.9546858
1.9633648
1.9691226
1.9938328
2.0043454
2.0043652
2.0184712
2.0320947
2.045776
2.0569882
2.0662014
2.0863683
2.086872
2.0934198
2.122592
2.134274
2.1549318
2.15637
2.1764894
2.1947885
2.2049687
2.2131386
2.213554
2.2328818
2.2329276
2.2396448
2.2489243
2.2535872
2.255215
2.2617152
2.2671669
2.270236
2.2761433
2.277913
2.2829251
2.2984126
2.3019562
2.306527
2.3128664
2.3315413
2.3527558
2.3596087
2.3683126
2.3865469
2.3949478
2.431466
2.436037
2.4381664
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hsa-miR-99b*
kshv-miR-K12-7
kshv-miR-K12-10b
ebv-miR-BHRF1-1
hsa-miR-135a*
hsa-miR-129-5p
hsa-miR-520e
hsa-miR-595
hsa-miR-557
hsa-miR-516a-5p
hsa-miR-134
hsa-miR-623
hsa-miR-1226*
hsa-miR-30c-2*
hsa-miR-188-5p
hiv1-miR-H1
hsa-miR-671-5p
hsa-miR-665
ebv-miR-BART17-3p
hsa-miR-526b
hsa-miR-187*
hsa-miR-520b
hsa-miR-370
hsa-miR-198
hsa-miR-424*
hsv1-miR-H1
hsa-miR-23a*
hsa-miR-765
hsa-miR-195*
hsa-miR-298
ebv-miR-BART16
hcmv-miR-US33-5p
hsa-miR-601
hsa-miR-518c*
hsa-miR-149*
hsa-miR-422a
hsa-miR-508-5p
hsa-miR-659
hsa-miR-30c-1*
2.4805777
2.485184
2.496572
2.5391386
2.5489833
2.5820384
2.5880218
2.6206522
2.6381726
2.6675062
2.6689427
2.674544
2.7421713
2.7536569
2.7614481
2.7727714
2.7831905
2.797279
2.7979145
2.8063183
2.8906887
2.9512932
2.9782224
3.0114477
3.0384455
3.0990682
3.1172748
3.2083426
3.2243376
3.3316617
3.3337893
3.4906313
3.545806
3.5828366
3.7758307
3.7929358
3.9209347
4.073046
4.319919
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