NMEICT PROJECT
Theory module: 03(MCQ)
MEASUREMENT OF COLOUR: BASIC OF COLORIMETER AND
SPECTROPHOTOMETER
1. Which of the following statement is incorrect regarding spectroscopy?
A. Absorption spectroscopy studies radiation absorbed at various
wavelengths
B. Emission spectroscopy observes light emitted by atoms excited by
radiation
C. One of the useful applications of light scattering spectroscopy is Raman
spectroscopy
D. Spectroscopy studies the way non-electromagnetic radiation interacts with
matter
2. The visible portion of the electromagnetic spectrum occurs between ___ nm and
___ nm.
A. 1, 10
B. 10, 300
C. 400, 740
D. 800, 1200
3. Which part of the spectrophotometer is adjusted to select the desired
wavelength?
A. Filter
B. Sample
C. Light source
D. Photo detector
4. In spectrophotometric experiments, the blank contains
A. Solute
B. Solvent
C. Light source
D. Absorption spectrum
5. Which of the following statement is incorrect regarding UV-visible
spectrophotometry?
A. It gives a measure of the amount of light absorbed by a sample
B. The sample is exposed to UV-visible light of different wavelengths
C. It is used to determine the concentration of a compound in solution
D. The amount of UV-visible light absorbed by a sample during UV-visible
spectrophotometry is called its absorbance
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NMEICT PROJECT
6. The distance travelled by light as it passes through a cuvette is called
A. Sample width
B. Focal length
C. Field length
D. Path length
7. Which of the following statements is incorrect with respect to Beer-Lambert law?
A. The amount of light absorbed is proportional to concentration of
absorbing substance
B. Io/I give transmittance
C. Transmittance measures the amount of light transmitted after passing
through the medium
D. Intensity of transmitted light decreases exponentially with increase in
concentration of absorbing substance
8. The plot of absorption verses wavelength for a particular compound is referred
to as the
A. Path length
B. Emission spectrum
C. Absorption spectrum
D. None of these
9. Which of the following is not a limitation of Beer- Lambert law?
A. Light must be of a narrow wavelength range and preferably
monochromatic
B. At high concentration, molecules may polymerize and thereby affecting
absorption of solution
C. Presence of fluorescent molecule in solution may interfere in the final
absorbance of solution
D. Beer- Lambert law does not form the quantitative basis for all types of
absorption spectroscopy
10. The photomultiplier tube is a widely used ---------------in UV-Vis spectroscopy
A.
B.
C.
D.
Wavelength selector
Detector
Signal processors
Cuvette
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NMEICT PROJECT
Theory module: 03 (Assignment)
MEASUREMENT OF COLOUR: BASIC OF COLORIMETER AND
SPECTROPHOTOMETER
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
12.
13.
14.
15.
What is the difference between spectrophotometer and colorimeter?
What is spectrophotometry?
What are the limitations of spectrophotometers?
What is colorimeter?
What is the definition of a color?
What is electromagnetic radiation?
Define Beer Lambert laws?
What are the limitations of Beer Lambert laws?
What are the important parts of colorimeter and spectrophotometer?
What are the source of light in spectrophotometer and colorimeter?
What is complementary color?
What is complementary wavelength?
What is monochrome and polychromatic light?
What are the types of cuvette used in spectrophotometer?
What are the types of detectors used in spectrophotometer?
DEVLOPMENT OF E-CONTENT ON FOUNDATION COURSE ON ANALYTICAL BIOCHEMISTRY AND SEPARATION TECHNIQUE
CHRIST COLLEGE, RAJKOT, GUJARAT.
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NMEICT PROJECT
Theory module: 03 (References)
MEASUREMENT OF COLOUR: BASIC OF COLORIMETER AND
SPECTROPHOTOMETER




Principles and techniques of biochemistry and molecular biology. Sixth edition.
Edited Keith Wilson & John Walker. Cambridge University press
Analytical biochemistry and separation techniques. – A laboratory manul for
B.Sc and M.Sc students. Palanivelu,
Introductory practical biochemistry. Edited by S.K Sawhney & Randhir Singh .
Narosa Publishing house Pvt Ltd.
http://teaching.shu.ac.uk/hwb/chemistry/tutorials/molspec/uvvisab3.htm
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NMEICT PROJECT
Theory module: 03 (Edutainment)
MEASUREMENT OF COLOUR: BASIC OF COLORIMETER AND
SPECTROPHOTOMETER
DEVLOPMENT OF E-CONTENT ON FOUNDATION COURSE ON ANALYTICAL BIOCHEMISTRY AND SEPARATION TECHNIQUE
CHRIST COLLEGE, RAJKOT, GUJARAT.
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NMEICT PROJECT
Across
41. the act of checking or adjusting (by
comparison with a standard) the
accuracy of a measuring instrument
16. When a parallel beam of
monochromatic light passes through an
absorbing medium, the amount of light
that is absorbed is directly proportional
to the number of light absorbing
molecules or the concentration of the
medium. Name the law
59.
Spectrophotometer
uses
the
deuterium or a ________________ lamp
for uv light.
5. A spectrophotometer uses a________to
separate light and a colorimeter uses
filters.
66. When a parallel beam of
monochromatic light passes through an
absorbing medium, the amount of light
that is absorbed is directly proportional
to the length the medium through which
the light passes. Give the name of this
law
84. _____________ spectrum consist of
wide range of radiations ranging from
gamma rays and x-rays from one end
and radio and television waves to other
end.
23. It is a device that measures the
intensity of the light before and after it
has passed through a coloured solution
74. Cuvettes for uv range is made up of
Down
1. When a coloured solution absorbs light
maximally at a particular wavelength then
that
wavelength
is
known
as
________________ wavelenght.
41. The containers for the sample and
reference solution in spectrophotometer
2. Which part of the spectrophotometer is
adjusted to select the desired wavelength?
12. It is an optical device that transmits a
mechanically selectable narrow band of
wavelengths of light or other radiation
chosen from a wider range of wavelengths
available at the input.
18. Light source in colorimeter =
______________ lamp
56. It converts the transmitted light energy
in to electrical energy.
81. The distance travelled by light as it
passes through a cuvette is called _________
length
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