Bioreagents
Fisher Bioreagents - P
DNA polymerase I source: recombinant E. coli strain
DNA polymerase, Taq
DNA Polymerase I is used for labelling DNA to high
specific radioactivity by nick translation.
32
DNA Polymerase I catalyses the polymerisation of nucleotides into duplex DNA in a 5’ to 3’
direction. Like many other DNA polymerases, the enzyme possesses a 3’ to 5’ exonuclease
activity or “proofreading” function. DNA Polymerase I also contains a 5’ to 3’ exonuclease
activity enabling the enzyme to replace nucleotides in the growing strand of DNA.
Unit definition: One unit is defined as the amount of enzyme required to catalyse the
incorporation of 10nmol of deoxyribonucleotides into TCA-insoluble form in 30min at 37°C
in 67mM potassium phosphate (pH7.5), 6.7mM MgCl2, 1mM DTT, 50µg/mL activated calf
thymus DNA and 33µM dATP, dCTP, dGTP and radiolabelled dTTP.
Catalogue No
BPE3200-1
Quantity
1,500 units
Price
72.10
Concentration: 5 to 10units/µL
Storage buffer components: 50mM Tris-HCI (pH7.5 at 25°C), 1mM DTT, 0.1mM EDTA and
50% (v/v) glycerol.
Taq DNA polymerase is a recombinant enzyme that is
licensed for use in PCR*. Provides superior performance
in routine PCR and RT-PCR. Capable of amplifying large
DNA target regions (up to 10kb). Well-suited for the
following PCR applications: STR, RAPD, AFLP, and SNP
analyses
Catalogue No
FB6000-10
FB6000-15
FB6000-25
FB6000-30
FB6000-35
FB6000-45
FB6000-50
FB6000-60
FB6000-65
FB6000-70
Quantity
100 units
500 units (5 x 100)
500 units
2,500 units (5 x 500)
2,500 units
100 units
500 units (5 x 100)
500 units
2,500 units (5 x 500)
2500 units
Buffers included
A
A
A
A
A
B
B
B
B
B
32
Price
24.30
96.90
92.20
383.50
375.30
25.00
100.50
94.50
389.40
375.30
Concentration: 5,000units/mL
Unit definition: One unit catalyses the incorporation of 10 nmole of total nucleotide into
acid-insoluable product in 30 minutes at 70°C utilising M13mp18 DNA as template.
Storage buffer: 50mM Tris-HCI (pH 7.5), 0.1mM EDTA, 5mM DTT, added stabilisers, and
50% glycerol
DNA polymerase I Klenow source: recombinant E. coli
strain
DNA polymerase I large (Klenow) fragment is used for
filling-in or labelling 3’ ends of double-stranded DNA,
second strand cDNA synthesis, and DNA sequencing by
the dideoxy method.
32
Product specification
Tested for DNase and Nickase contamination and a functional test for generating a PCR*
amplicon from human genomic DNA using primers for the p53 gene.
The DNA polymerase I large (Klenow) fragment lacks the 5’ to 3’ exonuclease activity of
intact DNA polymerase I but retains the 5’ to 3’ polymerase, the 3’ to 5’ exonuclease and
strand displacement activities.
Catalogue No
BPE3201-1
BPE3201-5
Quantity
150 units
500 units
Polymerase RNA
Price
56.70
123.60
First aid
Spillage
Storage
Concentration: 5 to 10units/µL
Storage buffer: 0.05M Tris-HCl (pH7.5), 1mM DTT, 0.1mM EDTA and 50% (v/v) glycerol.
RNA polymerase, SP6 source: recombinant E. coli strain
Product specification
Tested for: Activity, SDS-PAGE/purity, and endonuclease/nickase
SR6 RNA polymerase is used in synthesis of RNA
transcripts for hybridisation probes, in vitro translation,
RNase protection assays or RNA processing substrates.
DNA polymerase, T4 source: recombinant E. coli strain
T4 DNA polymerase is used to flush 5’ protruding
ends with labelled or unlabelled dNTPs and blunt 3’
overhangs. Also used in in vitro mutagenesis.
32
T4 DNA polymerase catalyses the 5’ to 3’ synthesis of DNA from a primed single-stranded
DNA template. Although possessing a potent 3’ to 5’ proofreading exonuclease, T4 DNA
polymerase contains no 5’ to 3’ exonuclease activity
Catalogue No
BPE3202-1
Quantity
100 units
Std.
C, G, H
-20°C
Price
47.30
Concentration: 5 to 10units/µL
Storage buffer: 200mM potassium phosphate (pH6.5), 2mM DTT and 50% glycerol.
One unit is defined as the amount of enzyme required to catalyse the incorporation of
10nmol of total nucleotide into an acid-insoluble form in 30min at 37°C
32
SP6 RNA polymerase is a DNA-dependent RNA polymerase that exhibits extremely high
specificity for bacteriophage SP6 promoter sequences. Using the SP6 enzyme, only SP6
DNA or DNA clones downstream from an SP6 promoter can serve as a template for RNA
synthesis. This enzyme will incorporate 32P, 35S and 3H nucleotide triphosphates.
Catalogue No
BPE3204-1
Quantity
1,000 units
Price
84.30
Concentration: 10 to 20units/µL
Storage buffer components: 20mM potassium phosphate buffer (pH7.7), 1mM EDTA,
10mM DTT, 0.1 M NaCl, 50% (v/v) glycerol and 0.1% Triton® X-100
Product specification
Tested for: Activity, SDS-PAGE/purity, dsDNase, RNase, endonuclease/nickase, and
specific performance tests
Product specification
Tested for: Activity, SDS-PAGE/purity, endonuclease/nickase, and specific performance
tests.
*Polymerase Chain Reaction (PCR) is a process covered by patents owned by
Hoffman-La Roche
1259