p-ERK
p-MEK
fail
fail
fail
5
no. 1
1788.2733
100
5
50
00
no. 2
B-Raf *
p-MEK
(U0126)
50
fail
30
00
pass
00
30
fail
00
30
100
100
100
100
50
50
50
50
00
pass
30
00
fail
30
20
00
fail
30
20
00
1290.1995
1788.5074
30
30
100
50
no. 3
10
00
no. 4
pass
30
00
10
pass
30
00
20
20
10
10
50
pass
100
50
00
pass
30
00
pass
30
00
50
fail
30
50
00
30
pass
00
30
324.6217
30
10
no. 5
no. 6
1671.8428
50
50
00
00
30
pass
30
00
50
pass
30
00
5
fail
30
00
100
100
100
50
50
50
00
fail
30
00
pass
30
00
1188.5518
1660.1634
30
30
100
50
50
no. 7
50
50
00
30
00
30
00
30
00
30
p-ERK
p-MEK
pass
no. 8
50
0
0
fail
30
0
pass
30
0
fail
30
0
50
0
fail
30
0
fail
30
0
0
fail
30
0
0
fail
30
0
0
fail
30
0
0
0
10
5
100
fail
30
0
0
100
fail
30
0
pass
30
0
71.5263
30
0
30
0
fail
30
0
0
87.734
30
0
156.2912
30
100
50
fail
30
0
0
15
fail
30
0
0
15
10
10
5
5
fail
30
0
100
50
0
100
fail
30
0
0
10
50
0
1786.1732
50
50
0
0
50
50
0
0
100
5
50
no. 14
30
50
10
50
0
no. 13
1224.5683
50
0
no. 12
0
50
no. 10
no. 11
0
100
50
50
50
0
665.4398
100
50
50
50
0
0
fail
100
50
100
no. 9
pass
100
B-Raf *
p-MEK
(U0126)
pass
30
30
0
0
10
5
0
0
fail
30
0
0
5
24.6046
30
5
0
30
0
0
30
0
0
30
p-ERK
p-MEK
fail
fail
50
0
0
100
no. 16
pass
30
0
0
pass
30
0
fail
30
0
0
50
0
fail
30
20
fail
0
0
40
50
0
1783.1002
100
50
50
50
0
fail
50
no. 15
B-Raf *
p-MEK
(U0126)
30
0
37.6744
30
20
0
fail
30
0
20
100
10
50
0
1791.9902
30
50
10
no. 17
0
0
fail
30
50
0
100
no. 19
no. 21
30
0
fail
30
0
pass
30
0
0
0
0
30
0
fail
30
0
pass
fail
30
0
0
fail
30
pass
30
0
0
30
0
0
fail
30
5
30
0
30
65.079
30
0
0
1242.3593
30
50
0
fail
30
15
5
74.2262
50
100
10
0
0
100
50
0
0
50
50
0
0
100
10
0
0
50
15
50
0
30
50
50
0
pass
50
100
no. 20
fail
50
0
0
50
no. 18
0
0
0
0
50
0
30
0
0
348.0896
30
30
p-ERK
pass
30
no. 22
p-MEK
fail
10
B-Raf *
p-MEK
(U0126)
pass
10
1665.1969
100
20
5
5
50
10
0
0
100
no. 23
pass
30
0
20
50
0
0
pass
30
50
pass
30
0
0
pass
30
0
pass
30
0
30
0
0
0
100
368.3738
30
50
0
10
5
0
fail
10
10
no. 24
0
20
10
0
0
fail
30
fail
30
0
0
50
1728.4403
30
5
0
fail
30
0
0
0
0
100
214.6307
30
50
50
no. 25
0
0
pass
30
50
0
100
no. 27
0
pass
30
20
no. 26
0
0
50
pass
30
0
0
50
0
pass
30
15
pass
30
0
10
5
5
0
pass
30
0
0
100
50
0
492.7197
30
50
15
10
0
100
20
0
0
328.4789
30
50
0
fail
30
0
0
pass
30
0
0
fail
30
0
0
100
876.2621
30
50
50
no. 28
0
0
30
0
50
0
30
0
50
0
30
0
0
30
p-ERK
p-MEK
pass
pass
15
B-Raf *
p-MEK
(U0126)
pass
15
1656.4467
100
50
no. 29
10
10
5
5
50
0
0
E1/4 (simulation)
E1 (simulation)
E1/4 (experiment)
E1 (experiment)
30
0
0
30
x-axis : time (min)
y-axis : Activation (% of total protein)
0
0
30
0
0
30