Suicide Ideation in the depression group (NBB)
A medical term for thoughts about or an unusual preoccupation with suicide. The range varies greatly from fleeting
to detailed planning, role playing, self-harm and attempts
Anterior Cingulate Cortex
GABBR2
GABRD
0.25
*
*
1.6
0.20
GluR1
0.24
**
0.22
1.4
0.15
0.20
1.2
0.10
0.18
1.0
0.05
0.16
0.8
0.00
NoSuicideIdeation
SuicideIdeation
0.14
NoSuicideIdeation
SuicideIdeation
NoSuicideIdeation
SuicideIdeation
DLPFC was also checked, however, none of the target genes were unchanged.
Juan Zhao and Dick Swaab, submitted
Microglia isolation procedure
• Mechanic & enzymatic dissociation
• Cell separation: Percoll gradient
• Take off myelin
• Collect cells from interface
• Stain cells with labeled antibodies:
o CD11b - PE
o CD45 - FITC
o CD15 - APC
• Selection using magnetic CD11b beads
Melief et al. Glia 2012
Isolation and phenotyping of pure primary human microglia
Peripheral inflammation impacts on microglia activation
Melief et al. Glia 2012
The Netherlands Brain Bank for Psychiatry
Phenotype & Characterize human post mortem microglia
Jeroen Melief, thesis 2014