Relative Expression Level
deltadeltaCT
A：Let-7a levels measured by quantitative real-time PCR
48 h post-transfection of Chol-let-7a or Chol-miRCtrl
12
10
8
HepG2
6
SMMC7721
4
2
0
Chol-let-7a
Chol-let-7a
Chol-miRCtrl
Chol-miRCtrl
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B：Expression of RAS proteins examined by western
blotting 48 h post transfection
HepG2
KRAS
HRAS
NRAS
β-actin
SMMC7721
C: Deregulated expression of k-ras, h-ras, and n-ras mRNAs as
determined by qRT-PCR 48 h post transfection
HepG2
let-7a target gene expression
delta CT
3.5
3
2.5
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2
Chol-miRCtrl
1.5
Chol-let-7a
1
0.5
0
k-ras
h-ras
n-ras
let-7a target gene expression
delta CT
SMMC7721
3.5
3
2.5
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2
Chol-miRCtrl
1.5
Chol-let-7a
1
0.5
0
k-ras
h-ras
n-ras