Supplementary Table S1. Multi-locus sequence analysis parameters
Gene
Primer
Sequence (5’- 3’)
Length
(bp)
16S rRNA 16F27
AGAGTTTGATCMTGGCTCAG
20
16R1492 TACGGYTACCTTGTTACGACTT
22
gyrB
UP1E
CAGGAAACAGCTATGACCAYGSNGGNGGNARTTYRA
36
APrU
TGTAAACGACGGCCAGTGCNGGRTCYTTYTCYTGRCA
37
M13R
CAGGAAACAGCTATGACC
18
M13(-21) TGTAAACGACGGCCAGT
17
rpoB
LAPS5
TGGCCGAGAACCAGTTCCGCGT
22
LAPS27
CGGCTTCGTCCAGCTTGTTCAG
22
rpoD
PsEG30F ATYGAAATCGCCAARCG
17
PsEG790R CGGTTGATKTCCTTGA
16
Reference
Fragment length (bp)
Lane, 1991
Lane, 1991
Yamamoto et al 2000
Yamamoto et al 2000
Yamamoto et al 2000
Yamamoto et al 2000
Tayeb et al 2005
Tayeb et al 2005
Mulet et al 2009
Mulet et al 2009
1465
966
1229
760
Supplementary Table S2. Growth of P. moraviensis stanleyae based on Colony Forming Units (CFU) and absorbance at OD600 in the
absence and presence of 10 mmol l-1 of Na2SeO3 and Na2SeO4
Time (h)
Control
(OD600)
Control
(CFU)*
SD
108 CFU /
ml
SeO32(CFU)a
SD
108 CFU /
ml
SeO42(OD600)
0
3
6
9
12
24
48
0
0.1645
0.668
1.060
1.266
1.343
1.396
ND
ND
78
102
203
282
296
ND
ND
10
13
21
12
16
ND
ND
3.9
5.1
10
14
15
ND
ND
18
55
135
133
134
ND
ND
5
8
18
8
11
ND
ND
0.9
2.8
6.8
6.7
6.7
0
0.207
0.689
1.012
1.181
1.292
1.433
*
CFU / plate (200 µl of 10-6 dilution); SD – standard deviation (triplicate); ND – not determined. Note: during the first 3 h of incubation no red Se(0) was
observed therefore the CFU approach was not employed.
Supplementary Figure S1. Red Se(0) produced under anaerobic conditions by the reduction of [SeO32-] = 10 mmol l-1 (described in
Materials and Methods). The red color starts to become apparent after 24 h of incubation.
References
Lane, D.J. (1991) 16S/23S rRNA sequencing. In Nucleic acid techniques in bacterial systematics in ed. Stackebrandt, E. and
Goodfellow, M. John Wiley and Sons, New York.
Mulet, M., Bennasar, A., Lalucat, J., and Garcia-Valdes, E. (2009) An rpoD-based PCR procedure for the identification of
Pseudomonas species and for their detection in environmental samples. Mol. Cell. Probes. 23, 140-147.
Tayeb, L., Ageron, E., Grimont, F., and Grimont, P.A.D. (2005) Molecular phylogeny of the genus Pseudomonas based on rpoB
sequences and application for the identification of isolates. Res. Microbiol. 156, 763-773.
Yamamoto, S., Kasai, H., Arnold, D.L., Jackson, R.W., Vivian, A., and Harayama, S. (2000) Phylogeny of the genus Pseudomonas:
intrageneric structure reconstructed from the nucleotide sequences of gyrB and rpoD genes. Microbiology 146, 2385-2394.